Nowadays, especially in the pharmaceutical industry, the need for Ultra Fast LC and Rapid Resolution is still growing due to the continuous demand for high throughput analysis in research
& development and quality control.
Especially for these Ultra Fast LC methods, YMC introduced YMC-UltraHT columns, which ideally matches the latest instrumentation technology for “extra” high pressure application e.g. with Agilent 1200™ series or Waters Acquity UPLC™.
As a column and bulk media supplier with many years of practical chromatographic experience, YMC found unacceptable that the use of novel separation media is often limited to dedicated equipment only – particularly with regard to the large installed base of “conventional” HPLC systems with a standard pressure rating of 5800 psi (400 bar), 40 MPa. For this reason, specifi cations for YMC-UltraHT columns are set to provide powerful chromatographic improvements concerning velocity
and resolution also under conventional operating conditions. Since YMC-UltraHT columns provides a substantially lower pressure drop than most competitive 2 μm or sub-2 μm media, high fl ow rates can be achieved without generating excessive back pressure and without the need for specialized equipment.
For effective high throughput separations, YMC offers a wide range of high performance HPLC columns which allow Ultra Fast analytical HPLC with conventional equipment. Due to the down-scalablility of the majority of YMC’s stationary phases, the time needed for a single analysis can so be reduced to less than 60 seconds, depending on the sample conditions.
General
YMC-UltraHT Pro C18
Since the introduction of the ProFamily series of phases, YMC-Pack Pro C18 has proved to be one of the fi rst choices for a wide range of HPLC applications in pharmaceutical and biotechnological research and production, where effi ciency and reliability are a major demand. YMC has transferred these outstanding qualities to Ultra Fast LC with the introduction of YMC-UltraHT Pro C18.
YMC-UltraHT Hydrosphere C18
In many cases, the separation of highly polar compounds requires highly aqueous mobile phase conditions to achieve suffi cient retention on the stationary phase. Conventional reversed phase selectivities do not give reproducible results under these conditions due to mainly collapse of the C18 chains. Therefore, YMC did developed Hydrosphere C18 in order of to overcome the loss in retention. Now, this outstanding chromatographic behaviour has been transferred to YMC-UltraHT Hydrosphere C18.
By comparing the relationship between particle size and the number of theoretical plates, the effect can be summarised as shown in the table below. Table 1 summarises comprehensively the effect of particle sizes on column effi ciency. Reduction of the particle size from 5 μm to 3 μm to 2 μm, allows the column length to be reduced by nearly two thirds without compromising effi ciency. This provides a corresponding reduction in separation time and eluent use.
|
| Column Length[mm] |
Number of Theoretical Plates
Particle Size |
5 μm |
3 μm |
2 μm |
150 |
80,000 |
120,000 |
190,000 |
100 |
67,000 |
80,000 |
160,000 |
50 |
33,000 |
40,000 |
80,000 |
|
