YMC Analytical Columns: Ultra Fast LC Columns: Downscale of Methods |
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- YMC Pack ProFamily chemistries, based on ultra high purity
silica,
provide excellent resolution for a wide range of analytes
- YMC-UltraHT LC Columns provide considerable
time saving without
resort to ultra high pressures
- YMC-UltraHT LC Columns achieve ultra fast
separations even with
conventional HPLC equipment
- Fully up- and down-scalable selectivity
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YMC-UltraHT Pro C18 |
YMC-UltraHT Hydrosphere C18 |
Particle size / µm |
2 |
2 |
Pore size / nm |
12 |
12 |
Surface area / m2g-1 |
330 |
330 |
Carbon content / % |
17 |
12 |
Recommended pH range |
2 - 8 |
2 - 8 |
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| Downscale of Methods |
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Due to the production processes used to manufacture YMC-Pack ProFamily, methods can be easily downscaled with unchanged selectivity. As the examples shown demonstrate, conventional HPLC methods can be transferred easily to Ultra Fast LC methods by choosing YMC-UltraHT columns to gain effi ciency and signifi cantly reduce analysis time.
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The application of HPLC to biologically relevant separations is an existing and rapidly growing field. YMC-UltraHT Pro C18 provides outstanding chromatgraphic performance, which is more than capable of meeting the challenge of peptide mapping, where a large number of peptide fragments are generated from enzymatic digestion. |
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| Why not take the pressure out of Fast LC! |
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The graph below shows the dependency of “Height Equivalent of the Theoretical Plate” (HETP) and the linear velocity in the presence of different organic solvents. When methanol is used, the optimum HETP is achieved within a different range of velocity compared to when acetonitrile is used due to their different viscosities. Therefore the optimum range of fl ow rate changes with the organic solvent.
The maximum resolution is obtained by optimising fl ow rate, temperature, and organic solvent in order to achieve the optimum back pressure. |
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With YMC-UltraHT Pro C18 you have all the efficiency you need to develop your Fast LC methods with none of the pressure or heat some would have you believe is essential! |
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* Source: Courtesy of YMC Co., Ltd. |
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