Agilent AdvanceBio Oligonucleotide Columns

AdvanceBio Oligonucleotide HPLC Columns from Agilent

To successfully separate trityl-off, deprotected oligonucleotides, you need columns that have high resolving power and are robust enough to withstand challenging conditions.

If your columns don’t provide enough resolution, you can’t be sure your results are accurate. If your columns are not robust, you will have to replace them frequently, which will disrupt your workflow and increase your costs. Agilent AdvanceBio oligonucleotide columns feature high efficiency, 2.7 µm superficially porous Poroshell particles. Using technology exclusive to Agilent, we chemically modify the particles to make them exceedingly resistant to high pH mobile phases. We also bond them with an end-capped C18 phase that delivers excellent selectivity for oligonucleotides. What’s more, we test every batch of AdvanceBio oligonucleotide media with a resolution standard to ensure consistent performance.

The family of AdvanceBio products are designed to deliver consistent, exceptional performance for the complete characterization of proteins, antibodies, conjugates, new biological entities and biopharmaceuticals.

AdvanceBio Oligonucleotide columns from Agilent are high-quality, high-resolution ion-pair reversed-phase (IP-RP) columns, which supports analytical characterisation and purification with seamless method scalability. These columns are designed to effectively separate trityl-off, deprotected oligonucleotides, with high resolving power and durability to withstand high pH and temperatures for superior separation and denaturing conditions for enhancing resolution and oligonucleotide separation.

The columns utilise high-efficiency, 2.7 and 4 μm superficially porous Poroshell particles. Exclusive Agilent technology allows for the chemical modification of these particles, increasing their resistance to high pH mobile phases. The particles are also bonded with an end-capped C18 phase, ensuring excellent selectivity for oligonucleotides. Each batch of AdvanceBio Oligonucleotide media is rigorously tested against a resolution standard to guarantee consistent performance.

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Buy

Part Number	Short Description	Phase	Particle Size	ID	Length	Pore Size	Type	Price
655750-702	AdvanceBio Oligonucleotide Column, 2.7um, 2.1 x 100mm	AdvanceBio Oligonucleotide	2.7 um	2.1 mm	100 mm	120 A	Column	£799.00
653750-702	AdvanceBio Oligonucleotide Column, 2.7um, 2.1 x 150mm	AdvanceBio Oligonucleotide	2.7 um	2.1 mm	150 mm	120 A	Column	£909.00
659750-702	AdvanceBio Oligonucleotide Column, 2.7um, 2.1 x 50mm	AdvanceBio Oligonucleotide	2.7 um	2.1 mm	50 mm	120 A	Column	£650.00
821725-921	AdvanceBio Oligonucleotide Fast Guard, 2.7um, 2.1 x 5mm, 3pk	AdvanceBio Oligonucleotide	2.7 um	2.1 mm	5 mm	120 A	Guard	£559.00
671150-702	AdvanceBio Oligonucleotide Column, 4um, 21.2 x 150mm	AdvanceBio Oligonucleotide	4 um	21.2 mm	150 mm	120 A	Column	£3,300.00
671050-702	AdvanceBio Oligonucleotide Column, 4um, 21.2 x 50mm	AdvanceBio Oligonucleotide	4 um	21.2 mm	50 mm	120 A	Column	£1,835.00
655950-702	AdvanceBio Oligonucleotide Column, 2.7um, 4.6 x 100mm	AdvanceBio Oligonucleotide	2.7 um	4.6 mm	100 mm	120 A	Column	£799.00
695971-702	AdvanceBio Oligonucleotide Column, 4um, 4.6 x 100mm	AdvanceBio Oligonucleotide	4 um	4.6 mm	100 mm	120 A	Column	£622.00
653950-702	AdvanceBio Oligonucleotide Column, 2.7um, 4.6 x 150mm	AdvanceBio Oligonucleotide	2.7 um	4.6 mm	150 mm	120 A	Column	£909.00
693971-702	AdvanceBio Oligonucleotide Column, 4um, 4.6 x 150mm	AdvanceBio Oligonucleotide	4 um	4.6 mm	150 mm	120 A	Column	£757.00
659950-702	AdvanceBio Oligonucleotide Column, 2.7um, 4.6 x 50mm	AdvanceBio Oligonucleotide	2.7 um	4.6 mm	50 mm	120 A	Column	£650.00
699971-702	AdvanceBio Oligonucleotide Column, 4um, 4.6 x 50mm	AdvanceBio Oligonucleotide	4 um	4.6 mm	50 mm	120 A	Column	£516.00
820750-921	AdvanceBio Oligonucleotide Fast Guard, 2.7um, 4.6 x 5mm, 3pk	AdvanceBio Oligonucleotide	2.7 um	4.6 mm	5 mm	120 A	Guard	£573.00
820750-941	AdvanceBio Oligonucleotide Fast Guard, 4um, 4.6 x 5mm, 3pk	AdvanceBio Oligonucleotide	4 um	4.6 mm	5 mm	120 A	Guard	£561.00
633750-702	AdvanceBio Oligonucleotide Column, 4um, 10 x 150mm	AdvanceBio Oligonucleotide	4 um	10.0 mm	150 mm	120 A	Column	£1,680.00
633950-702	AdvanceBio Oligonucleotide Column, 2.7um, 10 x 150mm	AdvanceBio Oligonucleotide	2.7 um	10.0 mm	150 mm	120 A	Column	£1,920.00
635750-702	AdvanceBio Oligonucleotide Column, 4um, 10 x 100mm	AdvanceBio Oligonucleotide	4 um	10.0 mm	100 mm	120 A	Column	£1,375.00
635950-702	AdvanceBio Oligonucleotide Column, 2.7um, 10 x 100mm	AdvanceBio Oligonucleotide	2.7 um	10.0 mm	100 mm	120 A	Column	£1,574.00
639750-702	AdvanceBio Oligonucleotide Column, 4um, 10 x 50mm	AdvanceBio Oligonucleotide	4 um	10.0 mm	50 mm	120 A	Column	£1,127.00
639950-702	AdvanceBio Oligonucleotide Column, 2.7um, 10 x 50mm	AdvanceBio Oligonucleotide	2.7 um	10.0 mm	50 mm	120 A	Column	£1,291.00
671100-702	AdvanceBio Oligonucleotide Column, 4um, 21.2 x 100mm	AdvanceBio Oligonucleotide	4 um	21.2 mm	100 mm	120 A	Column	£2,255.00

Features

Designed for ion-pair reversed-phase separation of the trityl-off, deprotected oligos using either TEAA or TEA-HFIP
Features the HPH-C18 robust column chemistry technology which is stable at elevated pH and temperatures
Streamlines scale up from analytical characterization to preparative purification
Every batch of AdvanceBio Oligonucleotide media is tested with the Agilent Oligonucleotide Resolution standard

Specifications

	AdvanceBio Oligonucleotide
Bonded Phase	C18
Particle Type	Superficially Porous
Endcapped	Double
Particle Size	2.7 µm, 4 µm
Pore Size	120 Å
Temp Limits	65°C
pH range	3 - 11

Literature

Agilent AdvanceBio Oligonucleotide Brochure (1.10 MB)

High resolution oligonucleotide separations with long column lifetime on HPLC and UHPLC systems

Agilent Bio HPLC Columns (26.00 MB)

Your essential resource for biomolecule analysis

Agilent AdvanceBio Oligonucleotide Columns

AdvanceBio Oligonucleotide HPLC Columns from Agilent

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