Agilent PolarGel
PolarGel HPLC Columns from Agilent
The PolarGel range is ideal for use with polar solvents, such as dimethyl formamide (DMF) and dimethyl sulfoxide (DMSO), and for solvent combinations such as tetrahydrofuran with water. These eluents are very useful in GPC/SEC to separate polar materials, such as polar resins, modified pollysaccharides or complex polar polymers that are difficult to analyze in traditional SEC solvents, such as tetrahydrofuran alone. PolarGel-L is used for low molecular weight polar polymers and PolarGel-M for high MW polar polymers.
With polar polymers, highly polar groups can lead to non-specific interactions and secondary separation mechanisms when using polar solvents and traditional non-polar styrene/divinylbenzene columns. Additives and/or column conditioning are normally required to reduce these interactions. PolarGel has no need for these interventions, and also avoids the interactions and secondary effects that produce chromatogram distortions.
These PolarGel "mixed bed" columns have a medium polarity surface and high mechanical stability. They are capable of operating in a wide range of solvents and solvent combinations, greatly enhancing your ability to analyze polar polymers that are not necessarily water soluble. PolarGel is available in two resolving ranges to meet your precise requirements.
- Proprietary surface chemistry prevents interaction in polar solvents
- Greatest flexibility with polar solvents, modifiers, and mixtures
- Excellent performance for polymers which dissolve in DMF, DMSO, NMP, DMAc, and THF - water mixtures
Column | Particle Size | Resolving Range (PEG/PEO in Water) | Max Temperature |
PolarGel-L | 8 µm | 100 to 60,000 | 90 ºC |
PolarGel-M | 8 µm | 1,000 to 500,000 | 90 ºC |