Mixed-Mode HPLC Columns

Mixed-mode HPLC columns combine multiple separation mechanisms – such as reversed phase, ion-exchange, and HILIC – into a single stationary phase, enabling the retention of ionic, polar, and hydrophobic analytes in one run. This powerful approach eliminates the need for column switching, ion-pairing reagents, derivatisation, or using columns in series, simplifying workflows and boosting productivity. 

With mixed-mode chromatography, selectivity can be easily optimised by adjusting mobile phase buffer concentration, pH, or solvent content, giving flexibility for both routine and complex separations. Well-suited for pharmaceutical compounds, metabolites, and other ionisable analytes, mixed-mode HPLC offers excellent compatibility with mass spectrometry while delivering high resolution, reproducibility, and efficiency.