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LC-MS Spectral Interpretation Level 2

1 Day

In this training course,  we look at the main fragmentation methods encountered in LC-MS and how we use fragment information in structural determination

This one day course covers advanced interpretation of LC-MS data. We explore multiple fragmentation mechanisms associated with collision-induced dissociation and the use of fragmentation in the structural elucidation of unknown (related) compounds, such as impurities and metabolic products.

The material is intensively workshop driven, building your experience of working with different types of fragmentation processes, applying these to compounds of known structure, then using these to interpret spectra of unknown related substances.


  • Revision of bonding theory
  • Ion formation and stabilisation mechanisms
  • Charge migration and charge retention fragmentation mechanisms
  • Using fragment information in structural elucidation


Here we explore the techniques used when working with fragments and applying these to structural determination

Revision of bonding theory

  • Covalent bonds
  • Lone pairs
  • Bond location references

Ion formation

  • Functional groups
  • Charge site initiation
  • Charge site stabilisation

Common fragmentation pathways

  • Charge migration mechanisms
  • Charge retention mechanisms

Using fragmentation for structural determination

All sections are extensively supported by workshops to illustrate and practice the techniques being learned.

This training is designed for delegates wishing to use LC-MS in the support of structural elucidation or those wishing to improve their confidence in working with data generated through product ion scanning

This is a challenging course, building on the skills developed in Level 1 and requires delegates to be experienced in LC-MS data acquisition and interpretation.

LC-MS Training

Interpretation of LC-MS Data – Level 1

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