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LC-MS Training

1 Day

We have distilled critical knowledge of liquid chromatography and mass spectrometry into a one day, interactive course

Learn the absolute essentials of the technique and, using our unique multi-media examples and tutorial exercises, implement new knowledge and ideas immediately.

The atmospheric pressure interface (API) is the core element of the course with the principles of operation, limitations and applicability fully explored. 

The course covers ion suppression, the use of electrospray or APCI and MS-MS data acquisition modes. Optimisation of interface and mass filter settings and how to best utilise the LC to speed up sample throughput will be discussed. 

All popular interface types and mass analysing equipment (quadrupole, TOF, ion trap, etc.) are comprehensively covered.

 
  • How modern API designs work and which one best fits your LC  and application requirements
  • How ions are produced and how to optimise LC mobile phase and interface parameters to achieve the highest sensitivity, accuracy  and reproducibility
  • The anatomy and working principles of quadrupoles, ion traps, time-of-flight detectors and more
  • The information that can be acquired in MS, MS-MS and MSn modes, and how to optimise instrument settings for high data quality

Solution Chemistry

  • LC vs MS sample considerations
  • Effect of pH
  • Buffers

Ionisation

  • The API source
  • Electrospray - principles & practice
  • APCI - principles & practice
  • Source maintenance
  • Useful application examples

Instrumentation

  • Ion Lenses
  • Cone voltage fragmentation (IS-CID)
  • Contamination and Cleaning
  • Vacuum Systems

Mass Analysers

  • Quadrupole theory
  • AMU Gain and Offset
  • Triple Quadrupole
  • Ion Traps
  • Time of Flight (TOF)
  • Orbitrap

Tuning and Calibration 

  • Tune Mixes
  • Positive / Negative Mode

LC-MS/MS Data Acquisition Modes

  • Scan vs SIM
  • Product ion scanning
  • Precursor ion scanning
  • Choosing precursor ions
  • Constant neutral loss
  • Data-dependent scanning
  • Introduction to MS interpretation
  • Establishing MRM method parameters

Users of LC-MS and LC-MS-MS equipment, or anyone interested in learning the analytical capabilities afforded by this type of instrument.

No previous knowledge of mass spectrometry is necessary, but an understanding of the fundamentals of liquid chromatography is beneficial.

Interpretation of LC-MS Data - Level 1

Interpretation of LC-MS Data - Level 2

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