Agilent Bio SEC Columns

Agilent Bio SEC HPLC Columns

Agilent Bio SEC offer fast, reliable, accurate performance for your biopharmaceutical analysis. They are easy to integrate into your workflow, and are available in a full range of pore sizes and dimensions to ensure the perfect separation every time.

Agilent Bio SEC-3

These columns are a breakthrough technology for size exclusion chromatography (SEC). They are packed with spherical, narrowly dispersed 3 µm silica particles coated with a proprietary hydrophilic layer. This thin polymeric layer is chemically bonded to pure, mechanically stable silica under controlled conditions, ensuring a highly efficient size exclusion particle.

Available in 100Å, 150Å and 300Å pore sizes to accommodate most peptide and protein size exclusion separations.

Agilent Bio SEC-5

These columns are packed with 5 µm silica particles coated with a proprietary, neutral, hydrophilic layer for maximum efficiency and stability. Our specially designed packing also promotes high pore volume, improving both peak capacity and resolution.

Available in 100Å, 150Å, 300Å, 500Å, 1000Å and 2000Å.

Agilent Bio SEC columns offer fast, reliable, accurate performance for your biopharmaceutical analysis. They are easy to integrate into your workflow and are available in a full range of pore sizes and dimensions to ensure the perfect separation every time.

Bio SEC-3 HPLC bio columns are a breakthrough technology for size exclusion chromatography. These bio columns are packed with spherical, narrowly dispersed 3 μm silica particles coated with a proprietary hydrophilic layer. This thin polymeric layer is chemically bonded to pure, mechanically stable silica under controlled conditions, ensuring an efficient size exclusion particle. Bio SEC-3 HPLC columns are available in 100Å, 150Å and 300Å pore sizes to accommodate most peptide and protein size exclusion separations.

Bio SEC-5 HPLC size exclusion chromatography columns are packed with 5 μm silica particles coated with a proprietary, neutral, hydrophilic layer for maximum efficiency and stability. Specially designed packing also promotes high pore volume, improving both peak capacity and resolution. Bio SEC-5 HPLC columns are available in 100 Å, 150 Å, 300 Å, 500 Å, 1000 Å, and 2000 Å.

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Buy

Part Number	Short Description	Particle Size	ID	Length	Pore Size	Type	Price
5190-6850	Agilent Bio SEC-3 100A 21.2 x 300mm Column	3 um	21.2 mm	300 mm	100 A	Column	£13,344.00
5190-6854	Agilent Bio SEC-3 100A 21.2 x 50mm Column	3 um	21.2 mm	50 mm	100 A	Column	£3,195.00
5190-2504	Agilent Bio SEC-3 100A 4.6 x 150mm Column	3 um	4.6 mm	150 mm	100 A	Column	£1,854.00
5190-2503	Agilent Bio SEC-3 100A 4.6 x 300mm Column	3 um	4.6 mm	300 mm	100 A	Column	£2,094.00
5190-6846	Agilent Bio SEC-3 100A 4.6 x 50mm Guard	3 um	4.6 mm	50 mm	100 A	Guard	£784.00
5190-2502	Agilent Bio SEC-3 100A 7.8 x 150mm Column	3 um	7.8 mm	150 mm	100 A	Column	£1,819.00
5190-2501	Agilent Bio SEC-3 100A 7.8 x 300mm Column	3 um	7.8 mm	300 mm	100 A	Column	£2,396.00
5190-2505	Agilent Bio SEC-3 100A 7.8 x 50mm Guard	3 um	7.8 mm	50 mm	100 A	Guard	£784.00
5190-6851	Agilent Bio SEC-3 150A 21.2 x 300mm Column	3 um	21.2 mm	300 mm	150 A	Column	£13,344.00
5190-6855	Agilent Bio SEC-3 150A 21.2 x 50mm Column	3 um	21.2 mm	50 mm	150 A	Column	£3,195.00
5190-2509	Agilent Bio SEC-3 150A 4.6 x 150mm Column	3 um	4.6 mm	150 mm	150 A	Column	£1,854.00
5190-2508	Agilent Bio SEC-3 150A 4.6 x 300mm Column	3 um	4.6 mm	300 mm	150 A	Column	£2,094.00
5190-6847	Agilent Bio SEC-3 150A 4.6 x 50mm Guard	3 um	4.6 mm	50 mm	150 A	Guard	£784.00
5190-2507	Agilent Bio SEC-3 150A 7.8 x 150mm Column	3 um	7.8 mm	150 mm	150 A	Column	£1,819.00
5190-2506	Agilent Bio SEC-3 150A 7.8 x 300mm Column	3 um	7.8 mm	300 mm	150 A	Column	£2,396.00
5190-2510	Agilent Bio SEC-3 150A 7.8 x 50mm Guard	3 um	7.8 mm	50 mm	150 A	Guard	£784.00
5190-6852	Agilent Bio SEC-3 300A 21.2 x 300mm Column	3 um	21.2 mm	300 mm	300 A	Column	£13,344.00
5190-6856	Agilent Bio SEC-3 300A 21.2 x 50mm Column	3 um	21.2 mm	50 mm	300 A	Column	£3,195.00
5190-2514	Agilent Bio SEC-3 300A 4.6 x 150mm Column	3 um	4.6 mm	150 mm	300 A	Column	£1,854.00
5190-2513	Agilent Bio SEC-3 300A 4.6 x 300mm Column	3 um	4.6 mm	300 mm	300 A	Column	£2,094.00
5190-6848	Agilent Bio SEC-3 300A 4.6 x 50mm Guard	3 um	4.6 mm	50 mm	300 A	Guard	£784.00
5190-2512	Agilent Bio SEC-3 300A 7.8 x 150mm Column	3 um	7.8 mm	150 mm	300 A	Column	£1,819.00
5190-2511	Agilent Bio SEC-3 300A 7.8 x 300mm Column	3 um	7.8 mm	300 mm	300 A	Column	£2,396.00
5190-2511K	Agilent Bio SEC-3 300A 7.8 x 300mm Method Validation Kit	3 um	7.8 mm	300 mm	300 A	Validation Kit	£7,187.00
5190-2515	Agilent Bio SEC-3 300A 7.8 x 50mm Guard	3 um	7.8 mm	50 mm	300 A	Guard	£784.00
5190-6867	Agilent Bio SEC-5 1000A 21.2 x 300mm Column	5 um	21.2 mm	300 mm	1000 A	Column	£9,477.00
5190-6873	Agilent Bio SEC-5 1000A 21.2 x 50mm Column	5 um	21.2 mm	50 mm	1000 A	Column	£2,328.00
5190-2539	Agilent Bio SEC-5 1000A 4.6 x 150mm Column	5 um	4.6 mm	150 mm	1000 A	Column	£1,093.00
5190-2538	Agilent Bio SEC-5 1000A 4.6 x 300mm Column	5 um	4.6 mm	300 mm	1000 A	Column	£1,404.00
5190-6861	Agilent Bio SEC-5 1000A 4.6 x 50mm Guard	5 um	4.6 mm	50 mm	1000 A	Guard	£549.00
5190-2537	Agilent Bio SEC-5 1000A 7.8 x 150mm Column	5 um	7.8 mm	150 mm	1000 A	Column	£1,310.00
5190-2536	Agilent Bio SEC-5 1000A 7.8 x 300mm Column	5 um	7.8 mm	300 mm	1000 A	Column	£1,715.00
5190-2540	Agilent Bio SEC-5 1000A 7.8 x 50mm Guard	5 um	7.8 mm	50 mm	1000 A	Guard	£566.00
5190-6863	Agilent Bio SEC-5 100A 21.2 x 300mm Column	5 um	21.2 mm	300 mm	100 A	Column	£9,247.00
5190-6869	Agilent Bio SEC-5 100A 21.2 x 50mm Column	5 um	21.2 mm	50 mm	100 A	Column	£2,340.00
5190-2519	Agilent Bio SEC-5 100A 4.6 x 150mm Column	5 um	4.6 mm	150 mm	100 A	Column	£1,153.00
5190-2518	Agilent Bio SEC-5 100A 4.6 x 300mm Column	5 um	4.6 mm	300 mm	100 A	Column	£1,424.00
5190-6857	Agilent Bio SEC-5 100A 4.6 x 50mm Guard	5 um	4.6 mm	50 mm	100 A	Guard	£551.00
5190-2517	Agilent Bio SEC-5 100A 7.8 x 150mm Column	5 um	7.8 mm	150 mm	100 A	Column	£1,238.00
5190-2516	Agilent Bio SEC-5 100A 7.8 x 300mm Column	5 um	7.8 mm	300 mm	100 A	Column	£1,723.00
5190-2520	Agilent Bio SEC-5 100A 7.8 x 50mm Guard	5 um	7.8 mm	50 mm	100 A	Guard	£562.00
5190-6864	Agilent Bio SEC-5 150A 21.2 x 300mm Column	5 um	21.2 mm	300 mm	150 A	Column	£9,247.00
5190-6870	Agilent Bio SEC-5 150A 21.2 x 50mm Column	5 um	21.2 mm	50 mm	150 A	Column	£2,340.00
5190-2524	Agilent Bio SEC-5 150A 4.6 x 150mm Column	5 um	4.6 mm	150 mm	150 A	Column	£1,153.00
5190-2523	Agilent Bio SEC-5 150A 4.6 x 300mm Column	5 um	4.6 mm	300 mm	150 A	Column	£1,424.00
5190-6858	Agilent Bio SEC-5 150A 4.6 x 50mm Guard	5 um	4.6 mm	50 mm	150 A	Guard	£551.00
5190-2522	Agilent Bio SEC-5 150A 7.8 x 150mm Column	5 um	7.8 mm	150 mm	150 A	Column	£1,238.00
5190-2521	Agilent Bio SEC-5 150A 7.8 x 300mm Column	5 um	7.8 mm	300 mm	150 A	Column	£1,723.00
5190-2525	Agilent Bio SEC-5 150A 7.8 x 50mm Guard	5 um	7.8 mm	50 mm	150 A	Guard	£562.00
5190-6868	Agilent Bio SEC-5 2000A 21.2 x 300mm Column	5 um	21.2 mm	300 mm	2000 A	Column	£10,811.00
5190-6874	Agilent Bio SEC-5 2000A 21.2 x 50mm Column	5 um	21.2 mm	50 mm	2000 A	Column	£2,546.00
5190-2544	Agilent Bio SEC-5 2000A 4.6 x 150mm Column	5 um	4.6 mm	150 mm	2000 A	Column	£1,093.00
5190-2543	Agilent Bio SEC-5 2000A 4.6 x 300mm Column	5 um	4.6 mm	300 mm	2000 A	Column	£1,404.00
5190-6862	Agilent Bio SEC-5 2000A 4.6 x 50mm Guard	5 um	4.6 mm	50 mm	2000 A	Guard	£549.00
5190-2542	Agilent Bio SEC-5 2000A 7.8 x 150mm Column	5 um	7.8 mm	150 mm	2000 A	Column	£1,310.00
5190-2541	Agilent Bio SEC-5 2000A 7.8 x 300mm Column	5 um	7.8 mm	300 mm	2000 A	Column	£1,715.00
5190-2545	Agilent Bio SEC-5 2000A 7.8 x 50mm Guard	5 um	7.8 mm	50 mm	2000 A	Guard	£566.00
5190-6865	Agilent Bio SEC-5 300A 21.2 x 300mm Column	5 um	21.2 mm	300 mm	300 A	Column	£9,247.00
5190-6871	Agilent Bio SEC-5 300A 21.2 x 50mm Column	5 um	21.2 mm	50 mm	300 A	Column	£2,340.00
5190-2529	Agilent Bio SEC-5 300A 4.6 x 150mm Column	5 um	4.6 mm	150 mm	300 A	Column	£1,153.00
5190-2528	Agilent Bio SEC-5 300A 4.6 x 300mm Column	5 um	4.6 mm	300 mm	300 A	Column	£1,424.00
5190-6859	Agilent Bio SEC-5 300A 4.6 x 50mm Guard	5 um	4.6 mm	50 mm	300 A	Guard	£551.00
5190-2527	Agilent Bio SEC-5 300A 7.8 x 150mm Column	5 um	7.8 mm	150 mm	300 A	Column	£1,238.00
5190-2526	Agilent Bio SEC-5 300A 7.8 x 300mm Column	5 um	7.8 mm	300 mm	300 A	Column	£1,723.00
5190-2530	Agilent Bio SEC-5 300A 7.8 x 50mm Guard	5 um	7.8 mm	50 mm	300 A	Guard	£562.00
5190-6866	Agilent Bio SEC-5 500A 21.2 x 300mm Column	5 um	21.2 mm	300 mm	500 A	Column	£9,477.00
5190-6872	Agilent Bio SEC-5 500A 21.2 x 50mm Column	5 um	21.2 mm	50 mm	500 A	Column	£2,328.00
5190-2534	Agilent Bio SEC-5 500A 4.6 x 150mm Column	5 um	4.6 mm	150 mm	500 A	Column	£1,093.00
5190-2533	Agilent Bio SEC-5 500A 4.6 x 300mm Column	5 um	4.6 mm	300 mm	500 A	Column	£1,404.00
5190-6860	Agilent Bio SEC-5 500A 4.6 x 50mm Guard	5 um	4.6 mm	50 mm	500 A	Guard	£549.00
5190-2532	Agilent Bio SEC-5 500A 7.8 x 150mm Column	5 um	7.8 mm	150 mm	500 A	Column	£1,310.00
5190-2531	Agilent Bio SEC-5 500A 7.8 x 300mm Column	5 um	7.8 mm	300 mm	500 A	Column	£1,715.00
5190-2535	Agilent Bio SEC-5 500A 7.8 x 50mm Guard	5 um	7.8 mm	50 mm	500 A	Guard	£566.00

Features

Exceptional loading capacity, stability, and reproducibility for size-based biomolecule separations
Sharper peaks, higher resolution, and better protein recovery
Faster separations than large-particle SEC columns
Compatibility with most aqueous buffers
Excellent stability in high-salt and low-salt conditions
Wide range of pore size options, including 1000 Å and 2000 Å gives options for everything from peptides to VLPs
Analytical and semi-prep dimensions for easy scale up or down

Specifications

	Bio SEC-3			Bio SEC-5
Pore Size	100 Å	150 Å	300 Å	100 Å	150 Å	300 Å	500 Å	1000 Å	2000 Å
Particle Size	3 µm	3 µm	3 µm	5 µm	5 µm	5 µm	5 µm	5 µm	5 µm
Particle Type	Fully Porous	Fully Porous	Fully Porous	Fully Porous	Fully Porous	Fully Porous	Fully Porous	Fully Porous	Fully Porous
Molecular Weight Lower Limit	100 Da	500 Da	5000 Da	100 Da	500 Da	5000 Da	15,000 Da	50,000 Da
Molecular Weight Upper Limit	100,000 Da	150,000 Da	1,250,000 Da	100,000 Da	150,000 Da	1,250,000 Da	5,000,000 Da	7,500,000 Da	>10,000,000 Da
Temperature Limit	80°C	80°C	80°C	80°C	80°C	80°C	80°C	80°C	80°C
pH range	2 - 8.5	2 - 8.5	2 - 8.5	2 - 8.5	2 - 8.5	2 - 8.5	2 - 8.5	2 - 8.5	2 - 8.5

Literature

Aggregate Analysis Application Compendium (6.82 MB)

Collection of Agilent app notes on aggregate/fragment analysis

Agilent Bio HPLC Columns (26.00 MB)

Your essential resource for biomolecule analysis