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Acclaim HILIC-10 HPLC Columns from Thermo Scientific

Separate highly hydrophilic molecules by hydrophilic interaction liquid chromatography using Thermo Scientific Acclaim HILIC-10 LC Columns.

These columns retain and separate highly polar molecules that are unretained by reversed-phase chromatography. Their high-purity, spherical, porous silica base is covalently modified with a proprietary hydrophilic layer. This chemistry enables the use of 5–20% aqueous mobile phase while maintaining affinity for polar analytes.

Sample throughput is increased without the need to evaporate and reconstitute samples dissolved in nonaqueous solvents.

Part Number Short Description Internal Diameter Particle Size Pore Size Phase Type Length Price Qty
074259 Acclaim HILIC-10, 3um, 2.1 x 150mm 2.1 mm 3 um 120 A HILIC Column 150 mm
£544.95
074263 Acclaim HILIC-10, 3um, 2.1 x 10mm, Guard Cartridge, 2/pk 2.1 mm 3 um 120 A HILIC Guard 10 mm
£152.25
  • Retains highly polar molecules that are not retained by reversed-phase chromatography
  • Unique selectivity, complementary to reversed-phase columns
  • Enhanced sensitivity for MS detection
  • Hydrolytically stable
  • Rugged column packing
  • Broad application range, including separation of hydrophilic drugs and drug metabolites
  Acclaim HILIC-10
Phase Proprietary Polar Group
Particle type Spherical, Fully Porous, Ultrapure Silica
Particle size 3 µm
Pore size 120 Å
Surface area 300 m2/g
Carbon Load 8%
pH range 2 - 8
LC Columns Technical Guide (13.60 MB)
Selection charts based on stationary phase use, analyte properties, LC-MS requirements or USP specifications
Acclaim Column Selection Guide (96.31 kB)
Quick guide to choosing your Acclaim phase by application
Analytical UHPLC and HPLC Columns Brochure (11.02 MB)
Introduction to the broad range of chemistries and column families from Thermo Scientific
LC Columns Catalogue 2019-2021 (23.33 MB)
Innovative columns for fast LC, analytical HPLC and UHPLC
Acclaim HILIC-10 Product Manual (419.06 kB)
User guide for the Acclaim HILIC-10 columns
Acclaim HILIC-10 Product Specification (956.71 kB)
Separating highly hydrophilic molecules
HILIC Separations Technical Guide (8.20 MB)
A Practical Guide to HILIC Mechanisms, Method Development and Troubleshooting
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