Acclaim HILIC-10 HPLC Columns from Thermo Scientific
Separate highly hydrophilic molecules by hydrophilic interaction liquid chromatography using Thermo Scientific Acclaim HILIC-10 LC Columns.
These columns retain and separate highly polar molecules that are unretained by reversed-phase chromatography. Their high-purity, spherical, porous silica base is covalently modified with a proprietary hydrophilic layer. This chemistry enables the use of 5–20% aqueous mobile phase while maintaining affinity for polar analytes.
Sample throughput is increased without the need to evaporate and reconstitute samples dissolved in nonaqueous solvents.
- Retains highly polar molecules that are not retained by reversed-phase chromatography
- Unique selectivity, complementary to reversed-phase columns
- Enhanced sensitivity for MS detection
- Hydrolytically stable
- Rugged column packing
- Broad application range, including separation of hydrophilic drugs and drug metabolites
| Acclaim HILIC-10 | |
| Phase | Proprietary Polar Group |
| Particle type | Spherical, Fully Porous, Ultrapure Silica |
| Particle size | 3 µm |
| Pore size | 120 Å |
| Surface area | 300 m2/g |
| Carbon Load | 8% |
| pH range | 2 - 8 |
LC Columns Technical Guide
(13.60 MB)
Selection charts based on stationary phase use, analyte properties, LC-MS requirements or USP specifications
Analytical UHPLC and HPLC Columns Brochure
(11.02 MB)
Introduction to the broad range of chemistries and column families from Thermo Scientific
HILIC Separations Technical Guide
(8.20 MB)
A Practical Guide to HILIC Mechanisms, Method Development and Troubleshooting