Acclaim Mixed-Mode

Acclaim Mixed-Mode HPLC Columns from Thermo Scientific

Acclaim Mixed-Mode HILIC-1

The Acclaim Mixed-Mode HILIC-1 column features a unique, high-efficiency, silica-based HPLC mixed-mode stationary phase that combines both reversed-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) properties. This combination allows both hydrophobic and hydrophilic interactions to be utilized to optimize separations.

The functional group is of a hydrophobic alkyl chain with a diol group at the terminus. This unique combination results in the adjustable selectivity, making Acclaim Mixed-Mode HILIC-1 separate mixtures that would be impossible for a C18 column. This column is suitable for a broad range of applications, including non-ionic ethoxylated surfactants, drug metabolites, lipids, polyethylene glycols (PEGs), ethoxylated surfactants, and more.

Acclaim Mixed-Mode WAX-1

The Acclaim Mixed-Mode WAX-1 is a novel, high-efficiency silica HPLC column that combines hydrophobic and weak anion exchange characteristics. Its unique chemistry results in a multimode separation mechanism that includes reversed-phase, anion exchange, and HILIC interactions. Selectivity can be adjusted by changing ionic strength, pH or organic solvent content.

Acclaim Mixed-Mode WCX-1

The Acclaim Mixed-Mode WCX-1 is a novel, high-efficiency, silica-based column, with a proprietary ligand with both hydrophobic and weak cation exchange properties. Selectivity of ionizable and neutral compounds can be controlled independently or simultaneously by tuning mobile phase ionic strength, pH or organic modifier. This column therefore can separate using multiple separation modes: reversed-phase, cation exchange, and normal-phase/HILIC and is recommended for a variety of industrial applications, including pharmaceutical, chemical, consumer products, foods and beverages.

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Part Number	Short Description	Internal Diameter	Particle Size	Pore Size	Phase	Type	Length	Price
064984	Acclaim Mixed-Mode WAX-1, 5um, 4.6 x 150mm	4.6 mm	5 um	120 A	Mixed-Mode WAX	Column	150 mm	£540.75
064985	Acclaim Mixed-Mode WAX-1, 5um, 4.6 x 250mm	4.6 mm	5 um	120 A	Mixed-Mode WAX	Column	250 mm	£633.15
066843	Acclaim Mixed-Mode HILIC-1, 5um, 4.6 x 150mm	4.6 mm	5 um	120 A	Mixed-Mode HILIC	Column	150 mm	£540.75
066844	Acclaim Mixed-Mode HILIC-1, 5um, 4.6 x 250mm	4.6 mm	5 um	120 A	Mixed-Mode HILIC	Column	250 mm	£633.15
066847	Acclaim Mixed-Mode HILIC-1, 5um, 2.1 x 150mm	2.1 mm	5 um	120 A	Mixed-Mode HILIC	Column	150 mm	£540.75
067084	Acclaim Mixed-Mode WAX-1, 5um, 2.1 x 150mm	2.1 mm	5 um	120 A	Mixed-Mode WAX	Column	150 mm	£540.75
068352	Acclaim Mixed-Mode WCX-1, 5um, 4.6 x 250mm	4.6 mm	5 um	120 A	Mixed-Mode WCX	Column	250 mm	£633.15
068353	Acclaim Mixed-Mode WCX-1, 5um, 4.6 x 150mm	4.6 mm	5 um	120 A	Mixed-Mode WCX	Column	150 mm	£544.95
068371	Acclaim Mixed-Mode WCX-1, 5um, 2.1 x 150mm	2.1 mm	5 um	120 A	Mixed-Mode WCX	Column	150 mm	£540.75
069686	Acclaim Mixed-Mode WAX-1, 5um, 2.1 x 10mm, Guard Cartridge, 2/pk	2.1 mm	5 um	120 A	Mixed-Mode WAX	Guard	10 mm	£145.95
069694	Acclaim Mixed-Mode HILIC-1, 5um, 2.1 x 10mm, Guard Cartridge, 2/pk	2.1 mm	5 um	120 A	Mixed-Mode HILIC	Guard	10 mm	£152.25
069704	Acclaim Mixed-Mode WAX-1, 5um, 4.6 x 10mm, Guard Cartridge, 2/pk	4.6 mm	5 um	120 A	Mixed-Mode WAX	Guard	10 mm	£145.95
069705	Acclaim Mixed-Mode WCX-1, 5um, 4.6 x 10mm, Guard Cartridge, 2/pk	4.6 mm	5 um	120 A	Mixed-Mode WCX	Guard	10 mm	£145.95
069706	Acclaim Mixed-Mode HILIC-1, 5um, 4.6 x 10mm, Guard Cartridge, 2/pk	4.6 mm	5 um	120 A	Mixed-Mode HILIC	Guard	10 mm	£145.95
070088	Acclaim Mixed-Mode WAX-1, 3um, 3 x 150mm	3.0 mm	3 um	120 A	Mixed-Mode WAX	Column	150 mm	£573.30
070089	Acclaim Mixed-Mode WAX-1, 3um, 2.1 x 150mm	2.1 mm	3 um	120 A	Mixed-Mode WAX	Column	150 mm	£573.30
070090	Acclaim Mixed-Mode HILIC-1, 3um, 3 x 150mm	3.0 mm	3 um	120 A	Mixed-Mode HILIC	Column	150 mm	£573.30
070091	Acclaim Mixed-Mode HILIC-1, 3um, 2.1 x 150mm	2.1 mm	3 um	120 A	Mixed-Mode HILIC	Column	150 mm	£573.30
070092	Acclaim Mixed-Mode WCX-1, 3um, 3 x 150mm	3.0 mm	3 um	120 A	Mixed-Mode WCX	Column	150 mm	£573.30
070093	Acclaim Mixed-Mode WCX-1, 3um, 2.1 x 150mm	2.1 mm	3 um	120 A	Mixed-Mode WCX	Column	150 mm	£573.30
071908	Acclaim Mixed-Mode WAX-1, 3um, 3 x 50mm	3.0 mm	3 um	120 A	Mixed-Mode WAX	Column	50 mm	£463.05
071909	Acclaim Mixed-Mode WAX-1, 5um, 3 x 10mm, Guard Cartridge, 2/pk	3.0 mm	5 um	120 A	Mixed-Mode WAX	Guard	10 mm	£145.95
071910	Acclaim Mixed-Mode WCX-1, 3um, 3 x 50mm	3.0 mm	3 um	120 A	Mixed-Mode WCX	Column	50 mm	£463.05
071911	Acclaim Mixed-Mode WCX-1, 5um, 3 x 10mm, Guard Cartridge, 2/pk	3.0 mm	5 um	120 A	Mixed-Mode WCX	Guard	10 mm	£145.95
071912	Acclaim Mixed-Mode HILIC-1, 3um, 3 x 50mm	3.0 mm	3 um	120 A	Mixed-Mode HILIC	Column	50 mm	£463.05
071913	Acclaim Mixed-Mode HILIC-1, 5um, 3 x 10mm, Guard Cartridge, 2/pk	3.0 mm	5 um	120 A	Mixed-Mode HILIC	Guard	10 mm	£145.95
088785	Acclaim Mixed-Mode WAX-1, 5um, 10 x 250mm	10.0 mm	5 um	120 A	Mixed-Mode WAX	Column	250 mm	£3,490.20

Features

Acclaim Mixed-Mode HILIC-1

Uniquely designed for both reversed-phase and HILIC operations
Can operate in both RP and HILIC modes
Retains highly polar molecules
Unique selectivity complementary to RP columns
Broader application range compared with conventional diol-based columns
High-efficiency column for high-resolution separations

Acclaim Mixed-Mode WAX-1

Designed for separating anionic molecules with powerful adjustable selectivity control
Selectivity orthogonal to reversed-phase (RP) columns
Ideal selectivity for anionic molecules
Excellent column efficiency and peak asymmetry
Multimode retention mechanisms: reversed-phase, weak anion exchange, and HILIC modes

Acclaim Mixed-Mode WCX-1

Designed for separating cationic molecules with adjustable selectivity control
Ideal selectivity for separating basic molecules
Selectivity complementary to C18 RP columns
Multimode separation mechanism: reversed-phase, weak cation exchange, anion-exclusion and HILIC

Specifications

	Acclaim Mixed-Mode HILIC-1	Acclaim Mixed-Mode WAX-1	Acclaim Mixed-Mode WCX-1
Phase	Proprietary alkyl diol	Proprietary alkyl	Proprietary alkyl carboxylic group
Particle type	Spherical, fully porous silica
Particle size	3, 5 µm
Pore size	120 Å
Surface area	300 m²/g
Endcapping	Proprietary
pH range	2.5 - 7.5
Temperature range	<50°C
USP code	-	L78	L85

Literature

Acclaim Mixed-Mode WCX-1 Product Specifications (503.17 kB)

For separating basic molecules

Acclaim Mixed-Mode Brochure (5.52 MB)

Columns for challenging separation needs

LC Columns Technical Guide (13.60 MB)

Selection charts based on stationary phase use, analyte properties, LC-MS requirements or USP specifications

Acclaim Column Selection Guide (96.31 kB)

Quick guide to choosing your Acclaim phase by application

Analytical UHPLC and HPLC Columns Brochure (11.02 MB)

Introduction to the broad range of chemistries and column families from Thermo Scientific

LC Columns Catalogue 2019-2021 (23.33 MB)

Innovative columns for fast LC, analytical HPLC and UHPLC

Acclaim Mixed-Mode HILIC-1 Product Specifications (330.66 kB)

A powerful tool for separating polar molecules

Acclaim-Mixed-Mode-WAX-1-Product-Specifications (474.21 kB)

Total control of selectivity

Acclaim Mixed-Mode HILIC-1 Product Manual (385.68 kB)

User guide for the Acclaim Mixed Mode HILIC-1 columns

Acclaim Mixed-Mode WAX-1 Product Manual (902.24 kB)

User guide for the Acclaim Mixed-Mode WAX-1 columns

Acclaim Mixed-Mode WCX-1 Product Manual (473.85 kB)

User guide for the Acclaim Mixed-Mode WCX-1 columns