Shodex Cation Exchange HPLC Columns

Shodex cation exchange HPLC columns are polymer-based and come with either strong or weak functional groups for the separation of biomolecules.

IEC SP-825 and IEC SP-FT 4A columns contain a strong cation exchange resin with a sulfopropyl functional group. These columns can be used at a wider pH range because the pKa of the ion exchange base is 2.3.

IEC CM-825 and Asahipak ES-502C are packed with a weak cation exchange resin with carboxymethyl as the functional group. These columns are suitable for the separation of basic proteins because the pKa of the ion exchange base is 5.7.

CXpak P-421 is a strong cation exchange column filled with spherical porous particles of styrene divinylbenzene copolymer. It is an ideal column for analyzing various amino acids. CXpak P-421 is used with an amino acid analyzer with post-column derivatization method.

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Part Number	Short Description	Phase	Particle Size	ID	Length	Pore Size	Type	Price
F7640001	Asahipak ES-502C 7C 7.6 x 100mm	Asahipak ES-502C	9 um	7.6 mm	100 mm	2000 A	Column	£1,171.69
F6354211	CXpak P-421S 4.6 x 150mm	CXpak P-421S	6 um	4.6 mm	150 mm		Column	£1,584.69
F6700210	CXpak P-G 4.6 x 10mm	CXpak P-421S	6 um	4.6 mm	10 mm		Guard	£549.99
F6110002	IEC CM-825 8 x 75mm	IEC CM-825	8 um	8.0 mm	75 mm	5000 A	Column	£951.03
F6118250	IEC SP-825 8 x 75mm	IEC SP-825	8 um	8.0 mm	75 mm	5000 A	Column	£951.03
F6113100	IEC SP-FT 4A 4.6 x 10mm	IEC SP-FT	2.7 um	4.6 mm	10 mm		Column	£1,050.36

Features

IEC SP-825 has a strong sulfopropyl groups suitable for analysing relatively high molecular weight compounds such as proteins, peptides, DNA, and RNA
IEC SP-FT 4A also has strong sulfopropyl groups with 2.7 µm non-porous base material for rapid analysis
IEC CM-825 is packed with weak cation exchange resin with carboxymethyl functional groups
Asahipak ES-502C is also has a weak cation exchange resin with carboxymethyl functional groups but with polyvinyl alcohol as the base material to offer different selectivity
CXpak P-421 is a strong cation exchange column filled with spherical porous particles of styrene divinylbenzene copolymer

Specifications

	IEC SP-825	IEC SP-FT 4A	IEC CM-825	Asahipak ES-502C 7C	CXpak P-421S
Functional Group	Sulfopropyl	Sulfopropyl	Carboxymethyl	Carboxymethyl	Sulfo (Na+)
Gel Material	Polyhydroxy-methacrylate	Polyhydroxy-methacrylate	Polyhydroxy-methacrylate	Polyvinyl alcohol	Styrene divinylbenzene copolymer
Particle Size	8 µm	2.7 µm	8 µm	9 µm	6 µm
Pore Size	5000 Å	-	5000 Å	2000 Å	-
Plates per Column	≥ 2000	≥ 20,000	≥ 2000	≥ 3300	≥ 3500
Temperature Range	10 - 50°C	5 - 45°C	10 - 50°C	10 - 50°C	15 - 63°C
pH Range	2 - 12	2 - 12	2 - 12	2 - 12	≥ 3
Salt Concentration	20 mM to 1.0 M	≤ 1.5 M	20 mM to 1.0 M	20 mM to 0.6 M	-
Ion Exchange Capacity	0.4 meq/g	0.2 meq/g	0.4 meq/g	0.55 meq/g	-
Pressure Limit	20 bar	200 bar	20 bar	20 bar	50 bar
Hardware	Stainless Steel	PEEK	Stainless Steel	Stainless Steel	Stainless Steel
Shipping Solvent	50 mM Na₂SO₄ aq.	20 mM 2-(N-Morpholino)ethanesulfonic acid buffer (pH 5.6)	50 mM Na₂SO₄ aq.	0.1 M Sodium phosphate buffer (pH 4.4)	H₂O
USP Designation	-	-	-	-	L22, L58

Literature