SiELC Primesep C HPLC Columns

SiELC Primesep C is a reverse-phase HPLC column with complex-forming groups embedded on long ligand chains. It improves retention of positively charged compounds by forming host-guest complexes and retains neutral compounds through a reversed phase mechanism. To facilitate the complex formation, the pH of the mobile phase should remain in the range of 3 - 7.

Typical applications include the separation of amines, sulfonium, phosphonium and metal ions. With the complex forming properties of Primesep C columns, primary amines are more retainable than secondary and tertiary amines. This extraordinary elution pattern allows tailoring the separation of amines to provide the most appropriate peak order for quantitation and preparative chromatography.

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Buy

Part Number	Short Description	Phase	USP	Particle Size	ID	Length	Pore Size	Type	Price
C-46.250.0310	Primesep C Column 4.6 x 250mm 3um 100A	Primesep C	L85	3 um	4.6 mm	250 mm	100 A	Column	£1,415.13
C-03.250.0510	Primesep C Column 0.25 x 250mm 5um 100A	Primesep C	L85	5 um	0.25 mm	250 mm	100 A	Column	£1,409.00
C-05.250.0510	Primesep C Column 0.5 x 250mm 5um 100A	Primesep C	L85	5 um	0.5 mm	250 mm	100 A	Column	£1,409.00
C-03.150.0510	Primesep C Column 0.25 x 150mm 5um 100A	Primesep C	L85	5 um	0.25 mm	150 mm	100 A	Column	£1,372.24
C-05.150.0510	Primesep C Column 0.5 x 150mm 5um 100A	Primesep C	L85	5 um	0.5 mm	150 mm	100 A	Column	£1,372.24
C-46.250.0510	Primesep C Column 4.6 x 250mm 5um 100A	Primesep C	L85	5 um	4.6 mm	250 mm	100 A	Column	£1,280.36
C-05.100.0310	Primesep C Column 0.5 x 100mm 3um 100A	Primesep C	L85	3 um	0.5 mm	100 mm	100 A	Column	£1,384.48
C-32.250.0310	Primesep C Column 3.2 x 250mm 3um 100A	Primesep C	L85	3 um	3.2 mm	250 mm	100 A	Column	£1,359.99
C-46.150.0310	Primesep C Column 4.6 x 150mm 3um 100A	Primesep C	L85	3 um	4.6 mm	150 mm	100 A	Column	£1,274.23
C-03.100.0510	Primesep C Column 0.25 x 100mm 5um 100A	Primesep C	L85	5 um	0.25 mm	100 mm	100 A	Column	£1,372.24
C-05.050.0310	Primesep C Column 0.5 x 50mm 3um 100A	Primesep C	L85	3 um	0.5 mm	50 mm	100 A	Column	£1,372.24
C-05.050.0510	Primesep C Column 0.5 x 50mm 5um 100A	Primesep C	L85	5 um	0.5 mm	50 mm	100 A	Column	£1,372.24
C-05.100.0510	Primesep C Column 0.5 x 100mm 5um 100A	Primesep C	L85	5 um	0.5 mm	100 mm	100 A	Column	£1,372.24
C-05.250.0310	Primesep C Column 0.5 x 250mm 3um 100A	Primesep C	L85	3 um	0.5 mm	250 mm	100 A	Column	£1,335.47
C-10.250.0310	Primesep C Column 1 x 250mm 3um 100A	Primesep C	L85	3 um	1.0 mm	250 mm	100 A	Column	£1,323.24
C-21.250.0310	Primesep C Column 2.1 x 250mm 3um 100A	Primesep C	L85	3 um	2.1 mm	250 mm	100 A	Column	£1,323.24
C-32.150.0310	Primesep C Column 3.2 x 150mm 3um 100A	Primesep C	L85	3 um	3.2 mm	150 mm	100 A	Column	£1,286.46
C-10.150.0310	Primesep C Column 1 x 150mm 3um 100A	Primesep C	L85	3 um	1.0 mm	150 mm	100 A	Column	£1,261.96
C-21.150.0310	Primesep C Column 2.1 x 150mm 3um 100A	Primesep C	L85	3 um	2.1 mm	150 mm	100 A	Column	£1,261.96
C-46.150.0510	Primesep C Column 4.6 x 150mm 5um 100A	Primesep C	L85	5 um	4.6 mm	150 mm	100 A	Column	£1,170.08
C-32.250.0510	Primesep C Column 3.2 x 250mm 5um 100A	Primesep C	L85	5 um	3.2 mm	250 mm	100 A	Column	£1,237.47
C-10.100.0310	Primesep C Column 1 x 100mm 3um 100A	Primesep C	L85	3 um	1.0 mm	100 mm	100 A	Column	£1,225.22
C-10.250.0510	Primesep C Column 1 x 250mm 5um 100A	Primesep C	L85	5 um	1.0 mm	250 mm	100 A	Column	£1,200.72
C-21.100.0310	Primesep C Column 2.1 x 100mm 3um 100A	Primesep C	L85	3 um	2.1 mm	100 mm	100 A	Column	£1,225.22
C-21.250.0510	Primesep C Column 2.1 x 250mm 5um 100A	Primesep C	L85	5 um	2.1 mm	250 mm	100 A	Column	£1,200.72
C-32.100.0310	Primesep C Column 3.2 x 100mm 3um 100A	Primesep C	L85	3 um	3.2 mm	100 mm	100 A	Column	£1,225.22
C-46.100.0310	Primesep C Column 4.6 x 100mm 3um 100A	Primesep C	L85	3 um	4.6 mm	100 mm	100 A	Column	£1,151.71
C-46.100.0510	Primesep C Column 4.6 x 100mm 5um 100A	Primesep C	L85	5 um	4.6 mm	100 mm	100 A	Column	£1,133.34
C-32.150.0510	Primesep C Column 3.2 x 150mm 5um 100A	Primesep C	L85	5 um	3.2 mm	150 mm	100 A	Column	£1,176.20
C-10.050.0310	Primesep C Column 1 x 50mm 3um 100A	Primesep C	L85	3 um	1.0 mm	50 mm	100 A	Column	£1,200.72
C-21.050.0310	Primesep C Column 2.1 x 50mm 3um 100A	Primesep C	L85	3 um	2.1 mm	50 mm	100 A	Column	£1,200.72
C-32.050.0310	Primesep C Column 3.2 x 50mm 3um 100A	Primesep C	L85	3 um	3.2 mm	50 mm	100 A	Column	£1,200.72
C-46.050.0310	Primesep C Column 4.6 x 50mm 3um 100A	Primesep C	L85	3 um	4.6 mm	50 mm	100 A	Column	£1,133.34
C-10.150.0510	Primesep C Column 1 x 150mm 5um 100A	Primesep C	L85	5 um	1.0 mm	150 mm	100 A	Column	£1,151.71
C-21.150.0510	Primesep C Column 2.1 x 150mm 5um 100A	Primesep C	L85	5 um	2.1 mm	150 mm	100 A	Column	£1,151.71
C-32.100.0510	Primesep C Column 3.2 x 100mm 5um 100A	Primesep C	L85	5 um	3.2 mm	100 mm	100 A	Column	£1,133.34
C-10.100.0510	Primesep C Column 1 x 100mm 5um 100A	Primesep C	L85	5 um	1.0 mm	100 mm	100 A	Column	£1,133.34
C-21.100.0510	Primesep C Column 2.1 x 100mm 5um 100A	Primesep C	L85	5 um	2.1 mm	100 mm	100 A	Column	£1,133.34
C-10.050.0510	Primesep C Column 1 x 50mm 5um 100A	Primesep C	L85	5 um	1.0 mm	50 mm	100 A	Column	£1,096.57
C-21.050.0510	Primesep C Column 2.1 x 50mm 5um 100A	Primesep C	L85	5 um	2.1 mm	50 mm	100 A	Column	£1,096.57
C-32.050.0510	Primesep C Column 3.2 x 50mm 5um 100A	Primesep C	L85	5 um	3.2 mm	50 mm	100 A	Column	£1,096.57
C-46.050.0510	Primesep C Column 4.6 x 50mm 5um 100A	Primesep C	L85	5 um	4.6 mm	50 mm	100 A	Column	£1,029.19
C-10.025.0310	Primesep C Column 1 x 25mm 3um 100A	Primesep C	L85	3 um	1.0 mm	25 mm	100 A	Column	£980.18
C-10.025.0510	Primesep C Column 1 x 25mm 5um 100A	Primesep C	L85	5 um	1.0 mm	25 mm	100 A	Column	£980.18
C-21.025.0310	Primesep C Column 2.1 x 25mm 3um 100A	Primesep C	L85	3 um	2.1 mm	25 mm	100 A	Column	£980.18
C-21.025.0510	Primesep C Column 2.1 x 25mm 5um 100A	Primesep C	L85	5 um	2.1 mm	25 mm	100 A	Column	£980.18
C-32.025.0310	Primesep C Column 3.2 x 25mm 3um 100A	Primesep C	L85	3 um	3.2 mm	25 mm	100 A	Column	£980.18
C-32.025.0510	Primesep C Column 3.2 x 25mm 5um 100A	Primesep C	L85	5 um	3.2 mm	25 mm	100 A	Column	£980.18
C-10.010.0310	Primesep C Column 1 x 10mm 3um 100A	Primesep C	L85	3 um	1.0 mm	10 mm	100 A	Column	£888.29
C-10.010.0510	Primesep C Column 1 x 10mm 5um 100A	Primesep C	L85	5 um	1.0 mm	10 mm	100 A	Column	£888.29
C-21.010.0310	Primesep C Column 2.1 x 10mm 3um 100A	Primesep C	L85	3 um	2.1 mm	10 mm	100 A	Column	£888.29
C-21.010.0510	Primesep C Column 2.1 x 10mm 5um 100A	Primesep C	L85	5 um	2.1 mm	10 mm	100 A	Column	£888.29
C-32.010.0310	Primesep C Column 3.2 x 10mm 3um 100A	Primesep C	L85	3 um	3.2 mm	10 mm	100 A	Column	£888.29
C-32.010.0510	Primesep C Column 3.2 x 10mm 5um 100A	Primesep C	L85	5 um	3.2 mm	10 mm	100 A	Column	£888.29
C-46.010.0310	Primesep C Column 4.6 x 10mm 3um 100A	Primesep C	L85	3 um	4.6 mm	10 mm	100 A	Column	£829.49
C-46.010.0510	Primesep C Column 4.6 x 10mm 5um 100A	Primesep C	L85	5 um	4.6 mm	10 mm	100 A	Column	£829.49
C-05.G.0310	Primesep C Guard Column 0.5 x 10mm 3um 100A, 2pk	Primesep C	L85	3 um	0.5 mm	10 mm	100 A	Guard	£441.08
C-10.G.0310	Primesep C Guard Column 1 x 10mm 3um 100A, 2pk	Primesep C	L85	3 um	1.0 mm	10 mm	100 A	Guard	£441.08
C-21.G.0310	Primesep C Guard Column 2.1 x 10mm 3um 100A, 2pk	Primesep C	L85	3 um	2.1 mm	10 mm	100 A	Guard	£441.08
C-32.G.0310	Primesep C Guard Column 3.2 x 10mm 3um 100A, 2pk	Primesep C	L85	3 um	3.2 mm	10 mm	100 A	Guard	£441.08
C-46.G.0310	Primesep C Guard Column 4.6 x 10mm 3um 100A, 2pk	Primesep C	L85	3 um	4.6 mm	10 mm	100 A	Guard	£441.08
C-10.G.0510	Primesep C Guard Column 1 x 10mm 5um 100A, 2pk	Primesep C	L85	5 um	1.0 mm	10 mm	100 A	Guard	£416.57
C-21.G.0510	Primesep C Guard Column 2.1 x 10mm 5um 100A, 2pk	Primesep C	L85	5 um	2.1 mm	10 mm	100 A	Guard	£416.57
C-32.G.0510	Primesep C Guard Column 3.2 x 10mm 5um 100A, 2pk	Primesep C	L85	5 um	3.2 mm	10 mm	100 A	Guard	£416.57
C-46.G.0510	Primesep C Guard Column 4.6 x 10mm 5um 100A, 2pk	Primesep C	L85	5 um	4.6 mm	10 mm	100 A	Guard	£416.57
C-100.050.0510	Primesep C Column 10 x 50mm 5um 100A	Primesep C	L85	5 um	10.0 mm	50 mm	100 A	Column	£2,839.72
C-100.100.0510	Primesep C Column 10 x 100mm 5um 100A	Primesep C	L85	5 um	10.0 mm	100 mm	100 A	Column	£3,394.20
C-100.150.0510	Primesep C Column 10 x 150mm 5um 100A	Primesep C	L85	5 um	10.0 mm	150 mm	100 A	Column	£3,965.51
C-100.250.0510	Primesep C Column 10 x 250mm 5um 100A	Primesep C	L85	5 um	10.0 mm	250 mm	100 A	Column	£5,192.15
C-220.050.0510	Primesep C Column 22 x 50mm 5um 100A	Primesep C	L85	5 um	22.0 mm	50 mm	100 A	Column	£5,091.33
C-220.100.0510	Primesep C Column 22 x 100mm 5um 100A	Primesep C	L85	5 um	22.0 mm	100 mm	100 A	Column	£5,847.47
C-220.150.0510	Primesep C Column 22 x 150mm 5um 100A	Primesep C	L85	5 um	22.0 mm	150 mm	100 A	Column	£7,275.73
C-220.250.0510	Primesep C Column 22 x 250mm 5um 100A	Primesep C	L85	5 um	22.0 mm	250 mm	100 A	Column	£9,157.67

Features

Primesep C is a reversed phase column with complex-forming groups embedded on long ligand chains
Improves retention of positively charged compounds by forming host-guest complexes
Retains neutral compounds through reverse-phase mechanism
Unique elution order of positively charged molecules in a chelating HPLC system
Offers mobile phase compatibility with LC-MS
Resistant to dewetting in 100% aqueous mobile phase
Scalable from capillary to prep
Guard columns connect directly to columns and do not require any additional holders

Specifications

	Primesep C
Mode	Mixed-Mode
Phase Type	Reverse-phase with complex-forming groups embedded on long ligand chains
Particle Type	Fully Porous
Particle Size	3 µm, 5 µm
Pore Size	100 Å
Temperature Range	0 - 50°C
pH Limit	1.5 - 7.0
Recommended pH Operating Range	2.0 - 6.5
Pressure Limit	345 bar

Literature

SiELC Primesep Brochure (1.10 MB)

Creating new dimensions in the world of chromatography

SiELC Primesep - Pharmaceutical Analysis Brochure (477.59 kB)

Solving problems of pharmaceutical HPLC analysis using Primesep columns

SiELC Primesep - Separation of Ions Brochure (293.32 kB)

New alternative to ion chromatography