Promix

Promix HPLC Columns from SiELC

Promix is designed for the separation of closely related proteins and peptides. It enables 2-D HPLC with a single column and can separate peptides that differ by only one amino acid. These columns are completely scalable from capillary to prep.

  • Promix AP is for small and medium size peptides under 3 kDa with PI value below 7.0
  • Promix MP is for medium size proteins and peptides from 1 to 10 kDa
  • Promix SP is for small peptides under 1 kDa with PI value below 6.0

Promix All pictures shown are for illustration purpose only. Actual product may vary.

Buy
Features

Promix AP is a column designed for short basic peptides. This phase allows the retention of peptides with several histidine and lysine residues. A pH gradient is the most convenient way to control retention of basic peptides on this column. Use a buffer with a neutral or slightly acidic pH (4.5 – 7) in the beginning of the gradient, and use a more acidic buffer (pH 2 – 3.5) in the end of the gradient elution.

  • Stationary phase for small and medium size peptides under 3 kDa with PI value below 7.0
  • Reverse phase and ion-exchange mechanism of interaction
  • Use acidic mobile phase pH from 2 to 5.5
  • Use ion strength at least 20 mM
  • 100 Å and 300 Å pore sizes are available

Promix MP is a column designed for medium size hydrophobic peptides. Typical applications include protein digest which increases peak capacity of the digest by 30 to 50%. This column offers alternative selectivity if compared with simple RP columns. Typically difficult to separate pairs of peptides can be resolved on this stationary phase.

  • Stationary phase for medium size proteins and peptides from 1 to 10 kDa
  • Broad pI value
  • Reverse phase and ion-exclusion mechanism of interaction
  • Use acidic mobile phase pH from 2 to 5.5
  • Use ion strength at least 1 mM
  • 300 Å and 800 Å pore sizes are available

Promix SP is a column for very short and/or very hydrophilic peptides. Typically it is difficult to retain such peptides on reversed phase columns. The Promix SP column offers an improved retention profile and alternative selectivity if compared with reversed phase separation.

  • Stationary phase for small peptides under 1 kDa with PI value below 6.0
  • Reverse phase and ion-exchange mechanism of interaction
  • Use acidic mobile phase pH from 2 to 5.5
  • Use ion strength at least 20 mM
  • 100 Å pore size
Specifications

Promix is an alternative chromatography technology for efficient resolution of peptides and proteins. The technology is based on a combination of two interactions – hydrophobic and ionic. This approach is possible due to a new type of separation media: a chemical combination of hydrophobic and ionic functional groups on a ligand bonded to a silica support. With this phase, unparalleled selectivity and peak capacity can be achieved. Independent adjustment of the amount of buffer and organic modifier creates an infinite number of separation conditions that are suitable for many types of biomolecules.

Literature
  • Promix Brochure

    Unsurpassed selectivity and peak capacity for peptides and proteins