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SiELC Promix AP Columns

SiELC Promix AP columns are suitable for the analysis of small and medium size peptides under 3 kDa with a PI value below 7.0. This mixed-mode analytical column has negatively charged acidic ion-pairing groups embedded within long ligand chains.

Promix AP is designed for analysis of short basic peptides. This phase allows the retention of peptides with several histidine and lysine residues. A pH gradient is the most convenient way to control retention of basic peptides. It is recommended to use a buffer with a neutral or slightly acidic pH (4.5 – 7) in the beginning of the gradient and use a more acidic buffer (pH 2 – 3.5) at the end of the gradient elution.

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Part Number Short Description Phase Particle Size ID Length Pore Size Type Price Qty
AP-05.100.0510 Promix AP Column 0.5 x 100mm 5um 100A Promix AP 5 um 0.5 mm 100 mm 100 A Column
£1,372.24
AP-05.150.0510 Promix AP Column 0.5 x 150mm 5um 100A Promix AP 5 um 0.5 mm 150 mm 100 A Column
£1,372.24
AP-05.250.0510 Promix AP Column 0.5 x 250mm 5um 100A Promix AP 5 um 0.5 mm 250 mm 100 A Column
£1,409.00
AP-46.250.0510 Promix AP Column 4.6 x 250mm 5um 100A Promix AP 5 um 4.6 mm 250 mm 100 A Column
£1,280.36
AP-46.150.0510 Promix AP Column 4.6 x 150mm 5um 100A Promix AP 5 um 4.6 mm 150 mm 100 A Column
£1,170.08
AP-32.250.0510 Promix AP Column 3.2 x 250mm 5um 100A Promix AP 5 um 3.2 mm 250 mm 100 A Column
£1,237.47
AP-10.250.0510 Promix AP Column 1 x 250mm 5um 100A Promix AP 5 um 1.0 mm 250 mm 100 A Column
£1,200.72
AP-21.250.0510 Promix AP Column 2.1 x 250mm 5um 100A Promix AP 5 um 2.1 mm 250 mm 100 A Column
£1,200.72
AP-46.100.0510 Promix AP Column 4.6 x 100mm 5um 100A Promix AP 5 um 4.6 mm 100 mm 100 A Column
£1,133.34
AP-32.150.0510 Promix AP Column 3.2 x 150mm 5um 100A Promix AP 5 um 3.2 mm 150 mm 100 A Column
£1,176.20
AP-10.150.0510 Promix AP Column 1 x 150mm 5um 100A Promix AP 5 um 1.0 mm 150 mm 100 A Column
£1,151.71
AP-21.150.0510 Promix AP Column 2.1 x 150mm 5um 100A Promix AP 5 um 2.1 mm 150 mm 100 A Column
£1,151.71
AP-32.100.0510 Promix AP Column 3.2 x 100mm 5um 100A Promix AP 5 um 3.2 mm 100 mm 100 A Column
£1,133.34
AP-10.100.0510 Promix AP Column 1 x 100mm 5um 100A Promix AP 5 um 1.0 mm 100 mm 100 A Column
£1,133.34
AP-21.100.0510 Promix AP Column 2.1 x 100mm 5um 100A Promix AP 5 um 2.1 mm 100 mm 100 A Column
£1,133.34
AP-10.050.0510 Promix AP Column 1 x 50mm 5um 100A Promix AP 5 um 1.0 mm 50 mm 100 A Column
£1,096.57
AP-21.050.0510 Promix AP Column 2.1 x 50mm 5um 100A Promix AP 5 um 2.1 mm 50 mm 100 A Column
£1,096.57
AP-32.050.0510 Promix AP Column 3.2 x 50mm 5um 100A Promix AP 5 um 3.2 mm 50 mm 100 A Column
£1,096.57
AP-46.050.0510 Promix AP Column 4.6 x 50mm 5um 100A Promix AP 5 um 4.6 mm 50 mm 100 A Column
£1,029.19
AP-10.025.0510 Promix AP Column 1 x 25mm 5um 100A Promix AP 5 um 1.0 mm 25 mm 100 A Column
£980.18
AP-21.025.0510 Promix AP Column 2.1 x 25mm 5um 100A Promix AP 5 um 2.1 mm 25 mm 100 A Column
£980.18
AP-32.025.0510 Promix AP Column 3.2 x 25mm 5um 100A Promix AP 5 um 3.2 mm 25 mm 100 A Column
£980.18
AP-10.010.0510 Promix AP Column 1 x 10mm 5um 100A Promix AP 5 um 1.0 mm 10 mm 100 A Column
£888.29
AP-21.010.0510 Promix AP Column 2.1 x 10mm 5um 100A Promix AP 5 um 2.1 mm 10 mm 100 A Column
£888.29
AP-32.010.0510 Promix AP Column 3.2 x 10mm 5um 100A Promix AP 5 um 3.2 mm 10 mm 100 A Column
£888.29
AP-46.010.0510 Promix AP Column 4.6 x 10mm 5um 100A Promix AP 5 um 4.6 mm 10 mm 100 A Column
£829.49
AP-10.G.0510 Promix AP Guard Column 1 x 10mm 5um 100A, 2pk Promix AP 5 um 1.0 mm 10 mm 100 A Guard
£416.57
AP-21.G.0510 Promix AP Guard Column 2.1 x 10mm 5um 100A, 2pk Promix AP 5 um 2.1 mm 10 mm 100 A Guard
£416.57
AP-32.G.0510 Promix AP Guard Column 3.2 x 10mm 5um 100A, 2pk Promix AP 5 um 3.2 mm 10 mm 100 A Guard
£416.57
AP-46.G.0510 Promix AP Guard Column 4.6 x 10mm 5um 100A, 2pk Promix AP 5 um 4.6 mm 10 mm 100 A Guard
£416.57
AP-100.050.0510 Promix AP Column 10 x 50mm 5um 100A Promix AP 5 um 10.0 mm 50 mm 100 A Column
£2,839.72
AP-100.100.0510 Promix AP Column 10 x 100mm 5um 100A Promix AP 5 um 10.0 mm 100 mm 100 A Column
£3,394.20
AP-100.150.0510 Promix AP Column 10 x 150mm 5um 100A Promix AP 5 um 10.0 mm 150 mm 100 A Column
£3,965.51
AP-100.250.0510 Promix AP Column 10 x 250mm 5um 100A Promix AP 5 um 10.0 mm 250 mm 100 A Column
£5,192.15
AP-220.050.0510 Promix AP Column 22 x 50mm 5um 100A Promix AP 5 um 22.0 mm 50 mm 100 A Column
£4,822.46
AP-220.100.0510 Promix AP Column 22 x 100mm 5um 100A Promix AP 5 um 22.0 mm 100 mm 100 A Column
£5,477.79
AP-220.150.0510 Promix AP Column 22 x 150mm 5um 100A Promix AP 5 um 22.0 mm 150 mm 100 A Column
£6,889.25
AP-220.250.0510 Promix AP Column 22 x 250mm 5um 100A Promix AP 5 um 22.0 mm 250 mm 100 A Column
£8,788.00
XAP-05.100.0510 Promix AP Column 0.5 x 100mm 5um 100A Promix AP 5 um 0.5 mm 100 mm 100 A Column
£1,788.82
XAP-05.100.0530 Promix AP Column 0.5 x 100mm 5um 300A Promix AP 5 um 0.5 mm 100 mm 300 A Column
£1,788.82
XAP-05.150.0510 Promix AP Column 0.5 x 150mm 5um 100A Promix AP 5 um 0.5 mm 150 mm 100 A Column
£1,752.05
XAP-05.150.0530 Promix AP Column 0.5 x 150mm 5um 300A Promix AP 5 um 0.5 mm 150 mm 300 A Column
£1,752.05
XAP-05.250.0510 Promix AP Column 0.5 x 250mm 5um 100A Promix AP 5 um 0.5 mm 250 mm 100 A Column
£1,752.05
XAP-05.250.0530 Promix AP Column 0.5 x 250mm 5um 300A Promix AP 5 um 0.5 mm 250 mm 300 A Column
£1,752.05
XAP-10.010.0510 Promix AP Column 1 x 10mm 5um 100A Promix AP 5 um 1.0 mm 10 mm 100 A Column
£1,047.56
XAP-10.010.0530 Promix AP Column 1 x 10mm 5um 300A Promix AP 5 um 1.0 mm 10 mm 300 A Column
£1,047.56
XAP-10.025.0510 Promix AP Column 1 x 25mm 5um 100A Promix AP 5 um 1.0 mm 25 mm 100 A Column
£1,133.34
XAP-10.025.0530 Promix AP Column 1 x 25mm 5um 300A Promix AP 5 um 1.0 mm 25 mm 300 A Column
£1,133.34
XAP-10.050.0510 Promix AP Column 1 x 50mm 5um 100A Promix AP 5 um 1.0 mm 50 mm 100 A Column
£1,249.72
XAP-10.050.0530 Promix AP Column 1 x 50mm 5um 300A Promix AP 5 um 1.0 mm 50 mm 300 A Column
£1,249.72
XAP-10.100.0510 Promix AP Column 1 x 100mm 5um 100A Promix AP 5 um 1.0 mm 100 mm 100 A Column
£1,286.46
XAP-10.100.0530 Promix AP Column 1 x 100mm 5um 300A Promix AP 5 um 1.0 mm 100 mm 300 A Column
£1,286.46
XAP-10.150.0510 Promix AP Column 1 x 150mm 5um 100A Promix AP 5 um 1.0 mm 150 mm 100 A Column
£1,335.47
XAP-10.150.0530 Promix AP Column 1 x 150mm 5um 300A Promix AP 5 um 1.0 mm 150 mm 300 A Column
£1,335.47
XAP-10.250.0510 Promix AP Column 1 x 250mm 5um 100A Promix AP 5 um 1.0 mm 250 mm 100 A Column
£1,359.99
XAP-10.250.0530 Promix AP Column 1 x 250mm 5um 300A Promix AP 5 um 1.0 mm 250 mm 300 A Column
£1,359.99
XAP-10.G.0510 Promix AP Guard Column 1 x 10mm 5um 100A, 2pk Promix AP 5 um 1.0 mm 10 mm 100 A Guard
£502.33
XAP-10.G.0530 Promix AP Guard Column 1 x 10mm 5um 300A, 2pk Promix AP 5 um 1.0 mm 10 mm 300 A Guard
£502.33
XAP-100.050.0510 Promix AP Column 10 x 50mm 5um 100A Promix AP 5 um 10.0 mm 50 mm 100 A Column
£2,958.04
XAP-100.050.0530 Promix AP Column 10 x 50mm 5um 300A Promix AP 5 um 10.0 mm 50 mm 300 A Column
£2,748.00
XAP-100.100.0510 Promix AP Column 10 x 100mm 5um 100A Promix AP 5 um 10.0 mm 100 mm 100 A Column
£3,535.64
XAP-100.100.0530 Promix AP Column 10 x 100mm 5um 300A Promix AP 5 um 10.0 mm 100 mm 300 A Column
£3,343.10
XAP-100.150.0510 Promix AP Column 10 x 150mm 5um 100A Promix AP 5 um 10.0 mm 150 mm 100 A Column
£4,130.75
XAP-100.150.0530 Promix AP Column 10 x 150mm 5um 300A Promix AP 5 um 10.0 mm 150 mm 300 A Column
£3,938.21
XAP-100.250.0510 Promix AP Column 10 x 250mm 5um 100A Promix AP 5 um 10.0 mm 250 mm 100 A Column
£5,408.48
XAP-100.250.0530 Promix AP Column 10 x 250mm 5um 300A Promix AP 5 um 10.0 mm 250 mm 300 A Column
£4,918.39
XAP-21.010.0510 Promix AP Column 2.1 x 10mm 5um 100A Promix AP 5 um 2.1 mm 10 mm 100 A Column
£1,047.56
XAP-21.010.0530 Promix AP Column 2.1 x 10mm 5um 300A Promix AP 5 um 2.1 mm 10 mm 300 A Column
£1,047.56
XAP-21.025.0510 Promix AP Column 2.1 x 25mm 5um 100A Promix AP 5 um 2.1 mm 25 mm 100 A Column
£1,133.34
XAP-21.025.0530 Promix AP Column 2.1 x 25mm 5um 300A Promix AP 5 um 2.1 mm 25 mm 300 A Column
£1,133.34
XAP-21.050.0510 Promix AP Column 2.1 x 50mm 5um 100A Promix AP 5 um 2.1 mm 50 mm 100 A Column
£1,249.72
XAP-21.050.0530 Promix AP Column 2.1 x 50mm 5um 300A Promix AP 5 um 2.1 mm 50 mm 300 A Column
£1,249.72
XAP-21.100.0510 Promix AP Column 2.1 x 100mm 5um 100A Promix AP 5 um 2.1 mm 100 mm 100 A Column
£1,286.46
XAP-21.100.0530 Promix AP Column 2.1 x 100mm 5um 300A Promix AP 5 um 2.1 mm 100 mm 300 A Column
£1,286.46
XAP-21.150.0510 Promix AP Column 2.1 x 150mm 5um 100A Promix AP 5 um 2.1 mm 150 mm 100 A Column
£1,335.47
XAP-21.150.0530 Promix AP Column 2.1 x 150mm 5um 300A Promix AP 5 um 2.1 mm 150 mm 300 A Column
£1,335.47
XAP-21.250.0510 Promix AP Column 2.1 x 250mm 5um 100A Promix AP 5 um 2.1 mm 250 mm 100 A Column
£1,359.99
XAP-21.250.0530 Promix AP Column 2.1 x 250mm 5um 300A Promix AP 5 um 2.1 mm 250 mm 300 A Column
£1,359.99
XAP-21.G.0510 Promix AP Guard Column 2.1 x 10mm 5um 100A, 2pk Promix AP 5 um 2.1 mm 10 mm 100 A Guard
£502.33
XAP-21.G.0530 Promix AP Guard Column 2.1 x 10mm 5um 300A, 2pk Promix AP 5 um 2.1 mm 10 mm 300 A Guard
£502.33
XAP-220.050.0510 Promix AP Column 22 x 50mm 5um 100A Promix AP 5 um 22.0 mm 50 mm 100 A Column
£5,303.46
XAP-220.050.0530 Promix AP Column 22 x 50mm 5um 300A Promix AP 5 um 22.0 mm 50 mm 300 A Column
£5,303.46
XAP-220.100.0510 Promix AP Column 22 x 100mm 5um 100A Promix AP 5 um 22.0 mm 100 mm 100 A Column
£6,091.10
XAP-220.100.0530 Promix AP Column 22 x 100mm 5um 300A Promix AP 5 um 22.0 mm 100 mm 300 A Column
£6,091.10
XAP-220.150.0510 Promix AP Column 22 x 150mm 5um 100A Promix AP 5 um 22.0 mm 150 mm 100 A Column
£5,601.02
XAP-220.150.0530 Promix AP Column 22 x 150mm 5um 300A Promix AP 5 um 22.0 mm 150 mm 300 A Column
£7,578.88
XAP-220.250.0510 Promix AP Column 22 x 250mm 5um 100A Promix AP 5 um 22.0 mm 250 mm 100 A Column
£9,539.23
XAP-220.250.0530 Promix AP Column 22 x 250mm 5um 300A Promix AP 5 um 22.0 mm 250 mm 300 A Column
£9,539.23
XAP-32.010.0510 Promix AP Column 3.2 x 10mm 5um 100A Promix AP 5 um 3.2 mm 10 mm 100 A Column
£1,047.56
XAP-32.010.0530 Promix AP Column 3.2 x 10mm 5um 300A Promix AP 5 um 3.2 mm 10 mm 300 A Column
£1,047.56
XAP-32.025.0510 Promix AP Column 3.2 x 25mm 5um 100A Promix AP 5 um 3.2 mm 25 mm 100 A Column
£1,133.34
XAP-32.025.0530 Promix AP Column 3.2 x 25mm 5um 300A Promix AP 5 um 3.2 mm 25 mm 300 A Column
£1,133.34
XAP-32.050.0510 Promix AP Column 3.2 x 50mm 5um 100A Promix AP 5 um 3.2 mm 50 mm 100 A Column
£1,249.72
XAP-32.050.0530 Promix AP Column 3.2 x 50mm 5um 300A Promix AP 5 um 3.2 mm 50 mm 300 A Column
£1,249.72
XAP-32.100.0510 Promix AP Column 3.2 x 100mm 5um 100A Promix AP 5 um 3.2 mm 100 mm 100 A Column
£1,298.73
XAP-32.100.0530 Promix AP Column 3.2 x 100mm 5um 300A Promix AP 5 um 3.2 mm 100 mm 300 A Column
£1,298.73
XAP-32.150.0510 Promix AP Column 3.2 x 150mm 5um 100A Promix AP 5 um 3.2 mm 150 mm 100 A Column
£1,347.74
XAP-32.150.0530 Promix AP Column 3.2 x 150mm 5um 300A Promix AP 5 um 3.2 mm 150 mm 300 A Column
£1,347.74
XAP-32.250.0510 Promix AP Column 3.2 x 250mm 5um 100A Promix AP 5 um 3.2 mm 250 mm 100 A Column
£1,372.24
XAP-32.250.0530 Promix AP Column 3.2 x 250mm 5um 300A Promix AP 5 um 3.2 mm 250 mm 300 A Column
£1,372.24
XAP-32.G.0510 Promix AP Guard Column 3.2 x 10mm 5um 100A, 2pk Promix AP 5 um 3.2 mm 10 mm 100 A Guard
£502.33
XAP-32.G.0530 Promix AP Guard Column 3.2 x 10mm 5um 300A, 2pk Promix AP 5 um 3.2 mm 10 mm 300 A Guard
£502.33
XAP-46.010.0510 Promix AP Column 4.6 x 10mm 5um 100A Promix AP 5 um 4.6 mm 10 mm 100 A Column
£992.41
XAP-46.010.0530 Promix AP Column 4.6 x 10mm 5um 300A Promix AP 5 um 4.6 mm 10 mm 300 A Column
£992.41
XAP-46.050.0510 Promix AP Column 4.6 x 50mm 5um 100A Promix AP 5 um 4.6 mm 50 mm 100 A Column
£1,176.20
XAP-46.050.0530 Promix AP Column 4.6 x 50mm 5um 300A Promix AP 5 um 4.6 mm 50 mm 300 A Column
£1,176.20
XAP-46.100.0510 Promix AP Column 4.6 x 100mm 5um 100A Promix AP 5 um 4.6 mm 100 mm 100 A Column
£1,292.61
XAP-46.100.0530 Promix AP Column 4.6 x 100mm 5um 300A Promix AP 5 um 4.6 mm 100 mm 300 A Column
£1,292.61
XAP-46.150.0510 Promix AP Column 4.6 x 150mm 5um 100A Promix AP 5 um 4.6 mm 150 mm 100 A Column
£1,323.24
XAP-46.150.0530 Promix AP Column 4.6 x 150mm 5um 300A Promix AP 5 um 4.6 mm 150 mm 300 A Column
£1,323.24
XAP-46.250.0510 Promix AP Column 4.6 x 250mm 5um 100A Promix AP 5 um 4.6 mm 250 mm 100 A Column
£1,507.00
XAP-46.250.0530 Promix AP Column 4.6 x 250mm 5um 300A Promix AP 5 um 4.6 mm 250 mm 300 A Column
£1,543.77
XAP-46.G.0510 Promix AP Guard Column 4.6 x 10mm 5um 100A, 2pk Promix AP 5 um 4.6 mm 10 mm 100 A Guard
£502.33
XAP-46.G.0530 Promix AP Guard Column 4.6 x 10mm 5um 300A, 2pk Promix AP 5 um 4.6 mm 10 mm 300 A Guard
£502.33
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Features
  • Stationary phase for small and medium size peptides under 3 kDa with pI value below 7.0
  • Reverse phase and ion-exchange mechanism of interaction
  • Use acidic mobile phase pH from 2 to 5.5
  • Use ion strength of at least 20 mM
  • 100 Å and 300 Å pore sizes are available
  • Completely scalable from capillary to prep
  • Guard columns connect directly to columns and do not require any additional holders
  • Promix range also includes Promix MP for medium sized peptides (1 to 10 kDa) and Promix SP for small peptides under 1 kDa with pI value below 6.0
Specifications
  Primesep AP
Phase Type Mixed-mode
Particle Type Fully Porous
Particle Size 5 µm
Pore Size 100 Å and 300 Å
Temperature Range 0 - 50°C
pH Limit 1.5 - 7.0
Recommended pH Operating Range 2.0 - 6.5
Pressure Limit 345 bar
Literature
SiELC Promix Columns Brochure (281.69 kB)
Unsurpassed selectivity and peak capacity for peptides and proteins
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