AdvanceBio Glycan Mapping
AdvanceBio Glycan Mapping HPLC Columns from Agilent
Agilent AdvanceBio Glycan Mapping Columns are designed and manufactured to deliver fast, high resolution, reproducible glycan identification using HILIC chromatography. AdvanceBio Glycan Mapping columns leverage technology that optimizes results for MS and fluorescence detection.
Choose from two UHPLC configurations: 2.7 µm superficially porous, for high resolution and lower backpressure, or 1.8 µm for highest resolution.
Order AdvanceBio Glycan Mapping columns with a selection of standards for performance testing and retention mapping of labeled and unlabeled glycans.
Our AdvanceBio N-glycan sample preparation kits provide all the components needed for the sample prep workflow with a comprehensive procedure to simplify the process.
- Speed: Obtain glycan maps in less than 10 minutes, superior results – in 40% less time than the competition
- High-resolution: Characterize both labeled and unlabeled glycans, and identify any glycosylation changes that occur due to process variables
- Flexibility: 2.7 µm superficially porous particles for high resolution with less backpressure. For UHPLC analysis 1.8 µm particles are an ideal choice
- Reproducibility: QA tested with a 2-AB labeled IgG N-linked glycan sample to ensure consistent quality and performance
Super-fast glycan analysis: Less than 10 minutes with 1.8 µm particles
Now it is possible to obtain glycan maps in less than 10 minutes with 1.8 µm particles. This gives you a distinct advantage during process development, when there are many samples to analyze and characterize.
Superior results – in 40% less time than the competition
| Conditions | |
| Column: | AdvanceBio Glycan Mapping Column 2.1 x 150 mm, 1.8 µm (p/n 859700-913) |
| Instrument: | Agilent 1290 Infinity (1260 Infinity FLD) |
| Column Temp: | 55 °C |
| Sample Thermostat: | 10 °C |
| Mobile Phase: | A: 100 mM NH4Formate, pH 4.5 B: ACN |
| Fluorescence Det: | Excitation = 260 Emission = 430 |
| Injection: | 2 µL in 70:30 ACN:100 mM NH4Formate |
| Sample: | Agilent 2-AB labeled N-linked Human IgG glycan library (p/n 5190-6996) |
| Time | %A | %B | Flow Rate (mL/min) |
| 0 | 20 | 80 | 0.5 |
| 25 | 40 | 60 | 0.5 |
| 26 | 100 | 0 | 0.5 |
| 27 | 40 | 80 | 0.5 |
The Agilent AdvanceBio Glycan Mapping column delivers better resolution, narrower bands, and higher peak capacity than the non-Agilent 1.7 µm column in a
2.1 x 150 mm configuration.
| AdvanceBio Glycan Mapping | Bonded Phase | Particle Size | Endcapped | pH Range | Temp Range | Pressure Limit |
| Amide HILIC | 1.8 µm, fully porous | No | 2 - 7 | 60 °C | 1200 bar | |
| Amide HILIC | 2.7 µm, superficially porous | No | 2 - 7 | 60 °C | 600 bar |
Agilent AdvanceBio Glycan Mapping Brochure
(1.78 MB)
Advance your Glycan Mapping - Agilent AdvanceBio glycan mapping solution
Agilent AdvanceBio Glycan Mapping 2.7 um Column Flyer
(1.10 MB)
Overview of the AdvanceBio Glycan Mapping 2.7 µm column
Agilent AdvanceBio Glycan Mapping 1.8 um Column Flyer
(1.28 MB)
Overview of the AdvanceBio Glycan Mapping 1.8 µm column
Agilent AdvanceBio Glycan Mapping Column User Guide
(1.40 MB)
User guide for the AdvanceBio Glycan Mapping column
Agilent AdvanceBio N-Glycan Sample Preparation User Guise for 96 Samples
(145.04 kB)
User Guide for AdvanceBio N-Glycan Sample Preparation for 96 Samples
Agilent AdvanceBio N-Glycan Sample Preparation User Guide for 24 Samples
(143.08 kB)
User guide for AdvanceBio N-Glycan Sample Preparation for 24 Samples