AdvanceBio Peptide Mapping

AdvanceBio Peptide Mapping HPLC Columns from Agilent

Agilent AdvanceBio Peptide Mapping columns are designed to provide high-resolution peptide maps for protein identification and determination of post translation modifications. These columns provide the ability for you to quickly resolve and identify amino acid substitutions/modifications in a protein primary sequence.

These advanced biocolumns are superficially porous and have a 120Å pore size which is optimum for the analysis of the peptides produced by enzymatic digest of proteins.

The 2.7 µm particle size enables UHPLC performance on HPLC systems, which delivers high resolution separations 2–3 times faster than with fully porous HPLC columns. Each batch of the AdvanceBio Peptide Mapping media is tested with a peptide mix to ensure suitability and reproducibility, and to provide greater confidence in the analytical data generated.

Use Agilent’s 10-Peptide Quality Control Standard, the same standard Agilent uses to QC its columns, to evaluate your column’s performance over its lifetime. It can be used for HPLC or LC/MS. Approximately 20 injections per vial. Order part number 5190-0583.

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Buy

Part Number	Short Description	Phase	Particle Size	ID	Length	Pore Size	Type	Price
5065-9925	AdvanceBio Peptide Mapping 0.075 x 150mm 2.7um Column	AdvanceBio Peptide Mapping	2.7 um	0.075 mm	150 mm	120 A	Column	£579.00
5065-9946	AdvanceBio Peptide Mapping 0.3 x 50mm 2.7um Column	AdvanceBio Peptide Mapping	2.7 um	0.3 mm	50 mm	120 A	Column	£354.00
863600-911	AdvanceBio Peptide Mapping 1 x 150mm 2.7um Column	AdvanceBio Peptide Mapping	2.7 um	1.0 mm	150 mm	120 A	Column	£889.00
655750-902	AdvanceBio Peptide Mapping 2.1 x 100mm 2.7um Column	AdvanceBio Peptide Mapping	2.7 um	2.1 mm	100 mm	120 A	Column	£678.00
653750-902	AdvanceBio Peptide Mapping 2.1 x 150mm 2.7um Column	AdvanceBio Peptide Mapping	2.7 um	2.1 mm	150 mm	120 A	Column	£739.00
651750-902	AdvanceBio Peptide Mapping 2.1 x 250mm 2.7um Column	AdvanceBio Peptide Mapping	2.7 um	2.1 mm	250 mm	120 A	Column	£871.00
851725-911	AdvanceBio Peptide Mapping 2.1mm 2.7um Guard 3pk	AdvanceBio Peptide Mapping	2.7 um	2.1 mm	5 mm	120 A	Guard	£532.00
655950-302	AdvanceBio Peptide Mapping 3 x 100mm 2.7um Column	AdvanceBio Peptide Mapping	2.7 um	3.0 mm	100 mm	120 A	Column	£677.00
653950-302	AdvanceBio Peptide Mapping 3 x 150mm 2.7um Column	AdvanceBio Peptide Mapping	2.7 um	3.0 mm	150 mm	120 A	Column	£739.00
853750-911	AdvanceBio Peptide Mapping 3mm 2.7um Guard 3pk	AdvanceBio Peptide Mapping	2.7 um	3.0 mm	5 mm	120 A	Guard	£532.00
653950-902	AdvanceBio Peptide Mapping 4.6 x 150mm 2.7um Column	AdvanceBio Peptide Mapping	2.7 um	4.6 mm	150 mm	120 A	Column	£739.00
850750-911	AdvanceBio Peptide Mapping 4.6mm 2.7um Guard 3pk	AdvanceBio Peptide Mapping	2.7 um	4.6 mm	5 mm	120 A	Guard	£532.00

Features

Greater analytical confidence - each batch of AdvanceBio Peptide Mapping media is tested with a rigorous peptide mix to ensure suitability and reproducibility, and to enable the identification of key peptides in complex peptide maps
Lower equipment cost - 4.6, 3, and 2.1 mm id columns give you UHPLC performance on any 400 bar HPLC instrument
Increased flexibility achieve increased MS sensitivity with formic acid mobile phases on any HPLC

Specifications

	Bonded Phase	Pore Size	Temp Limits*	pH Range	Endcapped
AdvanceBio Peptide Mapping	C18	120 Å	60 ºC	2 -8	Yes

* higher temperatures can be used but will reduce column lifetime

Literature

Agilent Bio HPLC Columns (26.00 MB)

Your essential resource for biomolecule analysis

Agilent AdvanceBio Peptide Mapping Brochure (2.93 MB)

Reduce peptide mapping time without losing resolution with Agilent AdvanceBio Peptide Mapping BioHPLC columns.

Agilent Reversed Phase protein Analysis (5.54 MB)

Protein Identification and Impurity Profiling using Reversed-Phase HPLC/UHPLC. Agilent biocolumns can boost your accuracy and productivity. This document covers complete portfolio of reversed-phase columns for protein identity applications.