AdvanceBio SEC

AdvanceBio SEC HPLC Columns from Agilent

AdvanceBio SEC columns deliver accurate, precise quantitation for mAb aggregation and protein analysis. These innovative size exclusion chromatography columns were designed and engineered by Agilent to improve lab productivity by providing robust, reliable methods that eliminate sample reanalysis. Consistent results are achieved from column to column, batch to batch, and lab to lab, ensuring that methods can be transferred across departments and locations to put an end to uncertainty.

The AdvanceBio SEC 1.9 µm column is designed to improve resolution and provided faster separation of mAb aggregates and fragments by using both HPLC and UHPLC. The optimized sub-two-micron particles are monodispersed and modified by unique hydrophilic bonding chemistry providing column reproducibility and stability, as well as preventing secondary interactions and protein adsorption. The AdvanceBio SEC 1.9 µm column is intended to meet the need for qualitative and quantitative critical quality attributes (CQA) analysis and method transferability across departments.

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Buy

Part Number	Short Description	Phase	Particle Size	ID	Length	Pore Size	Type	Price
PL1980-3250PK	AdvanceBio SEC 120A 2.1 x 150mm 1.9um PEEK	AdvanceBio SEC	1.9 um	2.1 mm	150 mm	120 A	PEEK Column	£1,405.00
PL1980-1250PK	AdvanceBio SEC 120A 2.1 x 50mm 1.9um PEEK	AdvanceBio SEC	1.9 um	2.1 mm	50 mm	120 A	PEEK Column	£1,299.00
PL1580-3250	AdvanceBio SEC 120A 4.6 x 150mm 1.9um	AdvanceBio SEC	1.9 um	4.6 mm	150 mm	120 A	Column	£1,816.00
PL1580-3250K	AdvanceBio SEC 120A 4.6 x 150mm 1.9um Method Validation Kit	AdvanceBio SEC	1.9 um	4.6 mm	150 mm	120 A	Validation Kit	£4,905.00
PL1580-1250	AdvanceBio SEC 120A 4.6 x 30 mm 1.9um Guard Column	AdvanceBio SEC	1.9 um	4.6 mm	30 mm	120 A	Guard	£552.00
PL1580-5250	AdvanceBio SEC 120A 4.6 x 300mm 1.9um	AdvanceBio SEC	1.9 um	4.6 mm	300 mm	120 A	Column	£2,582.00
PL1580-5250K	AdvanceBio SEC 120A 4.6 x 300mm 1.9um Method Validation Kit	AdvanceBio SEC	1.9 um	4.6 mm	300 mm	120 A	Validation Kit	£6,727.00
PL1580-3350	AdvanceBio SEC 130A 4.6 x 150mm 2.7um	AdvanceBio SEC	2.7 um	4.6 mm	150 mm	130 A	Column	£1,016.00
PL1580-5350	AdvanceBio SEC 130A 4.6 x 300mm 2.7um	AdvanceBio SEC	2.7 um	4.6 mm	300 mm	130 A	Column	£1,401.00
PL1580-1350	AdvanceBio SEC 130A 4.6 x 50mm 2.7um Guard	AdvanceBio SEC	2.7 um	4.6 mm	50 mm	130 A	Guard	£520.00
PL1180-3350	AdvanceBio SEC 130A 7.8 x 150mm 2.7um	AdvanceBio SEC	2.7 um	7.8 mm	150 mm	130 A	Column	£1,159.00
PL1180-5350	AdvanceBio SEC 130A 7.8 x 300mm 2.7um	AdvanceBio SEC	2.7 um	7.8 mm	300 mm	130 A	Column	£1,599.00
PL1180-1350	AdvanceBio SEC 130A 7.8 x 50mm 2.7um Guard	AdvanceBio SEC	2.7 um	7.8 mm	50 mm	130 A	Guard	£520.00
PL1980-3201PK	AdvanceBio SEC 200A 2.1 x 150mm 1.9um PEEK	AdvanceBio SEC	1.9 um	2.1 mm	150 mm	200 A	PEEK Column	£1,405.00
PL1980-1201PK	AdvanceBio SEC 200A 2.1 x 50mm 1.9um PEEK	AdvanceBio SEC	1.9 um	2.1 mm	50 mm	200 A	PEEK Column	£1,299.00
PL1580-3201	AdvanceBio SEC 200A 4.6 x 150mm 1.9um	AdvanceBio SEC	1.9 um	4.6 mm	150 mm	200 A	Column	£1,816.00
PL1580-5201	AdvanceBio SEC 200A 4.6 x 300mm 1.9um	AdvanceBio SEC	1.9 um	4.6 mm	300 mm	200 A	Column	£2,582.00
PL1580-1201	AdvanceBio SEC 200A 4.6 x 30mm 1.9um	AdvanceBio SEC	1.9 um	4.6 mm	30 mm	200 A	Column	£552.00
PL1580-3301	AdvanceBio SEC 300A 4.6 x 150mm 2.7um	AdvanceBio SEC	2.7 um	4.6 mm	150 mm	300 A	Column	£1,016.00
PL1580-5301	AdvanceBio SEC 300A 4.6 x 300mm 2.7um	AdvanceBio SEC	2.7 um	4.6 mm	300 mm	300 A	Column	£1,401.00
PL1580-1301	AdvanceBio SEC 300A 4.6 x 50mm 2.7um Guard	AdvanceBio SEC	2.7 um	4.6 mm	50 mm	300 A	Guard	£520.00
PL1180-3301	AdvanceBio SEC 300A 7.8 x 150mm 2.7um	AdvanceBio SEC	2.7 um	7.8 mm	150 mm	300 A	Column	£1,159.00
PL1180-5301	AdvanceBio SEC 300A 7.8 x 300mm 2.7um	AdvanceBio SEC	2.7 um	7.8 mm	300 mm	300 A	Column	£1,599.00
PL1180-1301	AdvanceBio SEC 300A 7.8 x 50mm 2.7um Guard	AdvanceBio SEC	2.7 um	7.8 mm	50 mm	300 A	Guard	£520.00

Features

High resolution, high-efficiency separation, and accurate quantitation for aggregates and fragments analysis
Prevented secondary interactions with mAb, ADC, and other proteins
Assured consistent column performance to eliminate rework
Enhanced column lifetime and productivity to meet robust CQA analysis
Specially designed standards for AdvanceBio SEC columns, providing optimal calibration and performance verification
AdvanceBio SEC 300 Å 2.7 µm is intended for separation protein ranging from 5,000 to 1.25 million Da including mAb fragments, mAb monomer, dimer, and high-level aggregates
AdvanceBio SEC 130 Å 2.7 µm is designed for small therapeutics molecular weight ranging from 100 to 120,000 Da
AdvanceBio SEC 200 Å 1.9 µm is suited for analyzing protein ranging from 2,000 to 700,000 Da including mAb monomer, dimmer, and LMW protein fragments
AdvanceBio SEC 120 Å 1.9 µm is best suited for analysis of peptides and small therapeutic proteins in the range 100 to 80,000 Da

Specifications

Pore Size	Particle Size	MW Range	pH Range	Max Pressure	Flow Rate
130 Å	2.7 µm	100 – 100,000	2 - 8.5	400 bar (typical operating pressure < 200 bar)	0.1 - 2 mL/min (7.8 mm id)
130 Å	2.7 µm	100 – 100,000	2 - 8.5	400 bar (typical operating pressure < 200 bar)	0.1 - 0.7 mL min (4.6 mm id)
300 Å	2.7 µm	5,000 – 1,250,000	2 - 8.5	400 bar (typical operating pressure < 200 bar)	0.1 - 2 mL/min (7.8 mm id)
300 Å	2.7 µm	5,000 – 1,250,000	2 - 8.5	400 bar (typical operating pressure < 200 bar)	0.1 - 0.7 mL/min (4.6 mm id)
200 Å	1.9 µm	2,000 – 700,000	2 - 8	620 bar	0.1 - 0.5 mL/min for 300 mm
200 Å	1.9 µm	2,000 – 700,000	2 - 8	620 bar	0.1 - 0.7 mL/min for 150 mm
120 Å	1.9 µm	100 – 80,000	2 - 8	620 bar	0.1 - 0.5 mL/min for 300 mm
120 Å	1.9 µm	100 – 80,000	2 - 8	620 bar	0.1 - 0.7 mL/min for 150 mm

Literature

Agilent Bio HPLC Columns (26.00 MB)

Your essential resource for biomolecule analysis

AdvanceBio SEC Brochure (1.68 MB)

Agilent AdvanceBio SEC Columns. End Uncertainty in Monoclonal Antibody Characterization.

AdvanceBio SEC 200A 1.9um Brochure (1.68 MB)

Information on the 200Å 1.9um AdvanceBio SEC columns

AdvanceBio SEC Flyer (1.06 MB)

Overview of the AdvanceBio SEC columns

End Uncertainty with AdvanceBio SEC Columns (696.16 kB)

Flyer illustrating real cost of your aggregation analysis method

Instrument Compatibility of AdvanceBio SEC Columns (1.42 MB)

Technical overview of instrument compatibility of AdvanceBio SEC columns

Advantages of AdvanceBio SEC Columns (526.95 kB)

Technical overview comparing columns from different vendors to improve data quality

Agilent Bio SEC Column Selection Guide (6.58 MB)

An overview on Agilent's SEC LC column portfolio, new technology, application examples and intrumentation

AdvanceBio SEC 120A 1.9um Brochure (1.60 MB)

Information on the 120Å 1.9um AdvanceBio SEC columns