Agilent Charge Variant Analysis Columns
During production and purification, antibodies can exhibit changes in charge heterogeneity as a result of amino acid substitutions, glycosylation, phosphorylation, and other post-translational or chemical modifications. Because these changes can impact stability and activity – or cause immunologically adverse reactions – the analysis of charge heterogeneity in monoclonal antibody (mAb) preparations is critical to biopharmaceutical manufacture.
In protein analysis, charge variations at a given pH indicate a change in the primary molecular structure – resulting in additional forms of the protein in question. These are called isoforms (or charge variants), and can be resolved by IEX chromatography. IEX is also useful as a preparative technique.
Because these changes can impact stability and activity – or cause immunologically adverse reactions – the analysis of charge variants is critical to biopharmaceuticals.
As a leading supplier to the biopharmaceutical industry, Agilent understands that quality and consistency are critical to providing safe, highly efficacious therapeutics. Agilent ion-exchange Bio HPLC columns offer the speed, resolution, and reproducibility you need to quickly and cost-effectively get life-changing products into the hands of those who need them.