Agilent Bio IEX

Agilent Bio IEX HPLC Columns

Agilent Bio Ion-Exchange HPLC columns are packed with polymeric, nonporous, ion-exchange particles. Bio Ion-Exchange Columns are designed for high resolution, high recovery and highly efficient separations of peptides, oligonucleotides and proteins. The Bio IEX family offers strong cation-exchange (SCX), weak cation-exchange (WCX), strong anion-exchange (SAX) and weak anion-exchange (WAX) phases. All Bio Ion-Exchange phases are available in 1.7, 3, 5 and 10 µm non-porous particles sizes.

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Buy

Part Number	Short Description	Phase	Particle Size	ID	Length	Pore Size	Type	Price
5190-2462	Agilent Bio SAX, NP1.7, 4 x 10mm, SS Guard	Agilent Bio SAX	1.7 um	4.0 mm	10 mm	Non-porous	SS Guard	£660.00
5190-2461	Agilent Bio SAX, NP1.7, 4.6 x 50mm, SS Column	Agilent Bio SAX	1.7 um	4.6 mm	50 mm	Non-porous	SS Column	£1,320.00
5190-2479	Agilent Bio SAX, NP10, 2.1 x 250mm, PK Column	Agilent Bio SAX	10 um	2.1 mm	250 mm	Non-porous	Peek Column	£1,665.00
5190-2480	Agilent Bio SAX, NP10, 2.1 x 50mm, PK Guard	Agilent Bio SAX	10 um	2.1 mm	50 mm	Non-porous	PEEK Guard	£722.00
5190-2474	Agilent Bio SAX, NP10, 4 x 10mm, SS Guard	Agilent Bio SAX	10 um	4.0 mm	10 mm	Non-porous	SS Guard	£472.00
5190-2475	Agilent Bio SAX, NP10, 4.6 x 250mm, PK Column	Agilent Bio SAX	10 um	4.6 mm	250 mm	Non-porous	Peek Column	£1,508.00
5190-2473	Agilent Bio SAX, NP10, 4.6 x 250mm, SS Column	Agilent Bio SAX	10 um	4.6 mm	250 mm	Non-porous	SS Column	£1,346.00
5190-2476	Agilent Bio SAX, NP10, 4.6 x 50mm, PK Guard	Agilent Bio SAX	10 um	4.6 mm	10 mm	Non-porous	PEEK Guard	£722.00
5190-2464	Agilent Bio SAX, NP3, 4 x 10mm, SS Guard	Agilent Bio SAX	3 um	4.0 mm	10 mm	Non-porous	SS Guard	£597.00
5190-2463	Agilent Bio SAX, NP3, 4.6 x 50mm, SS Column	Agilent Bio SAX	3 um	4.6 mm	50 mm	Non-porous	SS Column	£1,218.00
5190-2471	Agilent Bio SAX, NP5, 2.1 x 250mm, PK Column	Agilent Bio SAX	5 um	2.1 mm	250 mm	Non-porous	Peek Column	£1,917.00
5190-2472	Agilent Bio SAX, NP5, 2.1 x 50mm, PK Guard	Agilent Bio SAX	5 um	2.1 mm	50 mm	Non-porous	PEEK Guard	£942.00
5190-2466	Agilent Bio SAX, NP5, 4 x 10mm, SS Guard	Agilent Bio SAX	5 um	4.0 mm	10 mm	Non-porous	SS Guard	£534.00
5190-2467	Agilent Bio SAX, NP5, 4.6 x 250mm, PK Column	Agilent Bio SAX	5 um	4.6 mm	250 mm	Non-porous	Peek Column	£1,794.00
5190-2465	Agilent Bio SAX, NP5, 4.6 x 250mm, SS Column	Agilent Bio SAX	5 um	4.6 mm	250 mm	Non-porous	SS Column	£1,649.00
5190-2468	Agilent Bio SAX, NP5, 4.6 x 50mm, PK Guard	Agilent Bio SAX	5 um	4.6 mm	50 mm	Non-porous	PEEK Guard	£989.00
5190-2422	Agilent Bio SCX, NP1.7, 4 x 10mm, SS Guard	Agilent Bio SCX	1.7 um	4.0 mm	10 mm	Non-porous	SS Guard	£660.00
5190-2421	Agilent Bio SCX, NP1.7, 4.6 x 50mm, SS Column	Agilent Bio SCX	1.7 um	4.6 mm	50 mm	Non-porous	SS Column	£1,320.00
5190-2439	Agilent Bio SCX, NP10, 2.1 x 250mm, PK Column	Agilent Bio SCX	10 um	2.1 mm	250 mm	Non-porous	Peek Column	£1,665.00
5190-2440	Agilent Bio SCX, NP10, 2.1 x 50mm, PK Guard	Agilent Bio SCX	10 um	2.1 mm	50 mm	Non-porous	PEEK Guard	£722.00
5190-2434	Agilent Bio SCX, NP10, 4 x 10mm, SS Guard	Agilent Bio SCX	10 um	4.0 mm	10 mm	Non-porous	SS Guard	£472.00
5190-2435	Agilent Bio SCX, NP10, 4.6 x 250mm, PK Column	Agilent Bio SCX	10 um	4.6 mm	250 mm	Non-porous	Peek Column	£1,508.00
5190-2433	Agilent Bio SCX, NP10, 4.6 x 250mm, SS Column	Agilent Bio SCX	10 um	4.6 mm	250 mm	Non-porous	SS Column	£1,346.00
5190-2436	Agilent Bio SCX, NP10, 4.6 x 50mm, PK Guard	Agilent Bio SCX	10 um	4.6 mm	50 mm	Non-porous	PEEK Guard	£722.00
5190-2424	Agilent Bio SCX, NP3, 4 x 10mm, SS Guard	Agilent Bio SCX	3 um	4.0 mm	10 mm	Non-porous	SS Guard	£597.00
5190-2423	Agilent Bio SCX, NP3, 4.6 x 50mm, SS Column	Agilent Bio SCX	3 um	4.6 mm	50 mm	Non-porous	SS Column	£1,218.00
5190-2431	Agilent Bio SCX, NP5, 2.1 x 250mm, PK Column	Agilent Bio SCX	5 um	2.1 mm	250 mm	Non-porous	Peek Column	£1,917.00
5190-2432	Agilent Bio SCX, NP5, 2.1 x 50mm, PK Guard	Agilent Bio SCX	5 um	2.1 mm	50 mm	Non-porous	PEEK Guard	£942.00
5190-2426	Agilent Bio SCX, NP5, 4 x 10mm, SS Guard	Agilent Bio SCX	5 um	4.0 mm	10 mm	Non-porous	SS Guard	£534.00
5190-2427	Agilent Bio SCX, NP5, 4.6 x 250mm, PK Column	Agilent Bio SCX	5 um	4.6 mm	250 mm	Non-porous	Peek Column	£1,794.00
5190-2425	Agilent Bio SCX, NP5, 4.6 x 250mm, SS Column	Agilent Bio SCX	5 um	4.6 mm	250 mm	Non-porous	SS Column	£1,649.00
5190-2428	Agilent Bio SCX, NP5, 4.6 x 50mm, PK Guard	Agilent Bio SCX	5 um	4.6 mm	50 mm	Non-porous	PEEK Guard	£989.00
5190-2482	Agilent Bio WAX, NP1.7, 4 x 10mm, SS Guard	Agilent Bio WAX	1.7 um	4.0 mm	10 mm	Non-porous	SS Guard	£660.00
5190-2481	Agilent Bio WAX, NP1.7, 4.6 x 50mm, SS Column	Agilent Bio WAX	1.7 um	4.6 mm	50 mm	Non-porous	SS Column	£1,320.00
5190-2499	Agilent Bio WAX, NP10, 2.1 x 250mm, PK Column	Agilent Bio WAX	10 um	2.1 mm	250 mm	Non-porous	Peek Column	£1,665.00
5190-2500	Agilent Bio WAX, NP10, 2.1 x 50mm, PK Guard	Agilent Bio WAX	10 um	2.1 mm	50 mm	Non-porous	PEEK Guard	£722.00
5190-2494	Agilent Bio WAX, NP10, 4 x 10mm, SS Guard	Agilent Bio WAX	10 um	4.0 mm	10 mm	Non-porous	SS Guard	£472.00
5190-2495	Agilent Bio WAX, NP10, 4.6 x 250mm, PK Column	Agilent Bio WAX	10 um	4.6 mm	250 mm	Non-porous	Peek Column	£1,508.00
5190-2493	Agilent Bio WAX, NP10, 4.6 x 250mm, SS Column	Agilent Bio WAX	10 um	4.6 mm	250 mm	Non-porous	SS Column	£1,346.00
5190-2496	Agilent Bio WAX, NP10, 4.6 x 50mm, PK Guard	Agilent Bio WAX	10 um	4.6 mm	10 mm	Non-porous	PEEK Guard	£722.00
5190-2484	Agilent Bio WAX, NP3, 4 x 10mm, SS Guard	Agilent Bio WAX	3 um	4.0 mm	10 mm	Non-porous	SS Guard	£597.00
5190-6875	Agilent Bio WAX, NP3, 4.6 x 150mm, SS Column	Agilent Bio WAX	3 um	4.6 mm	150 mm	Non-porous	SS Column	£1,492.00
5190-2483	Agilent Bio WAX, NP3, 4.6 x 50mm, SS Column	Agilent Bio WAX	3 um	4.6 mm	50 mm	Non-porous	SS Column	£1,218.00
5190-2491	Agilent Bio WAX, NP5, 2.1 x 250mm, PK Column	Agilent Bio WAX	5 um	2.1 mm	250 mm	Non-porous	Peek Column	£1,917.00
5190-2492	Agilent Bio WAX, NP5, 2.1 x 50mm, PK Guard	Agilent Bio WAX	5 um	2.1 mm	50 mm	Non-porous	PEEK Guard	£989.00
5190-2486	Agilent Bio WAX, NP5, 4 x 10mm, SS Guard	Agilent Bio WAX	5 um	4.0 mm	10 mm	Non-porous	SS Guard	£534.00
5190-2487	Agilent Bio WAX, NP5, 4.6 x 250mm, PK Column	Agilent Bio WAX	5 um	4.6 mm	250 mm	Non-porous	Peek Column	£1,794.00
5190-2485	Agilent Bio WAX, NP5, 4.6 x 250mm, SS Column	Agilent Bio WAX	5 um	4.6 mm	250 mm	Non-porous	SS Column	£1,649.00
5190-2488	Agilent Bio WAX, NP5, 4.6 x 50mm, PK Guard	Agilent Bio WAX	5 um	4.6 mm	10 mm	Non-porous	PEEK Guard	£989.00
5190-2442	Agilent Bio WCX, NP1.7, 4 x 10mm, SS Guard	Agilent Bio WCX	1.7 um	4.0 mm	10 mm	Non-porous	SS Guard	£660.00
5190-2441	Agilent Bio WCX, NP1.7, 4.6 x 50mm, SS Column	Agilent Bio WCX	1.7 um	4.6 mm	50 mm	Non-porous	SS Column	£1,320.00
5190-2459	Agilent Bio WCX, NP10, 2.1 x 250mm, PK Column	Agilent Bio WCX	10 um	2.1 mm	250 mm	Non-porous	Peek Column	£1,665.00
5190-2460	Agilent Bio WCX, NP10, 2.1 x 50mm, PK Guard	Agilent Bio WCX	10 um	2.1 mm	50 mm	Non-porous	PEEK Guard	£722.00
5190-2454	Agilent Bio WCX, NP10, 4 x 10mm, SS Guard	Agilent Bio WCX	10 um	4.0 mm	10 mm	Non-porous	SS Guard	£472.00
5190-2455	Agilent Bio WCX, NP10, 4.6 x 250mm, PK Column	Agilent Bio WCX	10 um	4.6 mm	250 mm	Non-porous	Peek Column	£1,508.00
5190-2453	Agilent Bio WCX, NP10, 4.6 x 250mm, SS Column	Agilent Bio WCX	10 um	4.6 mm	250 mm	Non-porous	SS Column	£1,346.00
5190-2456	Agilent Bio WCX, NP10, 4.6 x 50mm, PK Guard	Agilent Bio WCX	10 um	4.6 mm	50 mm	Non-porous	PEEK Guard	£722.00
5190-2444	Agilent Bio WCX, NP3, 4 x 10mm, SS Guard	Agilent Bio WCX	3 um	4.0 mm	10 mm	Non-porous	SS Guard	£597.00
5190-2443	Agilent Bio WCX, NP3, 4.6 x 50mm, SS Column	Agilent Bio WCX	3 um	4.6 mm	50 mm	Non-porous	SS Column	£1,218.00
5190-2451	Agilent Bio WCX, NP5, 2.1 x 250mm, PK Column	Agilent Bio WCX	5 um	2.1 mm	250 mm	Non-porous	Peek Column	£1,917.00
5190-2452	Agilent Bio WCX, NP5, 2.1 x 50mm, PK Guard	Agilent Bio WCX	5 um	2.1 mm	50 mm	Non-porous	PEEK Guard	£942.00
5190-2447	Agilent Bio WCX, NP5, 4.6 x 250mm, PK Column	Agilent Bio WCX	5 um	4.6 mm	250 mm	Non-porous	Peek Column	£1,794.00
5190-2445	Agilent Bio WCX, NP5, 4.6 x 250mm, SS Column	Agilent Bio WCX	5 um	4.6 mm	250 mm	Non-porous	SS Column	£1,649.00
5190-2448	Agilent Bio WCX, NP5, 4.6 x 50mm, PK Guard	Agilent Bio WCX	5 um	4.6 mm	50 mm	Non-porous	PEEK Guard	£989.00
5190-2446	Agilent Bio WCX, NP5, 4x 10mm, SS Guard	Agilent Bio WCX	5 um	4.0 mm	10 mm	Non-porous	SS Guard	£534.00
5190-6882	DG-Agilent Bio SAX, NP5, 10 x 250mm, SS Column	Agilent Bio SAX	5 um	10.0 mm	250 mm	Non-porous	SS Column	£5,983.00
5190-6883	DG-Agilent Bio SAX, NP5, 21.2 x 250mm, SS Column	Agilent Bio SAX	5 um	21.2 mm	250 mm	Non-porous	SS Column	£19,430.00
5190-6878	DG-Agilent Bio SCX, NP5, 10 x 250mm, SS Column	Agilent Bio SCX	5 um	10.0 mm	250 mm	Non-porous	SS Column	£5,983.00
5190-6879	DG-Agilent Bio SCX, NP5, 21.2 x 250mm, SS Column	Agilent Bio SCX	5 um	21.2 mm	250 mm	Non-porous	SS Column	£19,430.00
5190-6876	DG-Agilent Bio WAX, NP5, 10 x 250mm, SS Column	Agilent Bio WAX	5 um	10.0 mm	250 mm	Non-porous	SS Column	£5,983.00
5190-6877	DG-Agilent Bio WAX, NP5, 21.2 x 250mm, SS Column	Agilent Bio WAX	5 um	21.2 mm	250 mm	Non-porous	SS Column	£19,430.00
5190-6880	DG-Agilent Bio WCX, NP5, 10 x 250mm, SS Column	Agilent Bio WCX	5 um	10.0 mm	250 mm	Non-porous	SS Column	£5,983.00
5190-6881	DG-Agilent Bio WCX, NP5, 21.2 x 250mm, SS Column	Agilent Bio WCX	5 um	21.2 mm	250 mm	Non-porous	SS Column	£19,430.00

Features

Highly crosslinked and rigid nonporous poly(styrene divinylbenzene) (PS/DVB) particles are grafted with a hydrophilic, polymeric layer, eliminating nonspecific binding
Uniform, densely packed ion-exchange functional groups are chemically bonded to the hydrophilic layer (multiple ion-exchange groups per anchoring) to increase column capacity
Particles, coating and bonding are resistant to high pressures, promoting higher resolution and faster separations
Multiple ion-exchange groups are captured on one anchoring to increase capacity

Specifications

Column phase	SCX (Strong cation-exchange) – SO₃H WCX (Weak cation-exchange) – COOH SAX (Strong anion-exchange) – N(CH₃)₃ WAX (Weak anion-exchange) – N(C₂H₅)₂
Packing	Non-porous, poly(styrene divinylbenzene) (PS/DVB), grafted hydrophilic coating and bonded with a uniform, ion-exchange layer
Particle size	1.7, 3, 5 and 10 µm
Pore structure	Non-porous
pH stability	2 - 12
Operating temperature limit	80 ºC

Column hardware operating pressure limit	600 bar (8,700 psi) for stainless steel column hardware 400 bar (5,800 psi) for PEEK column hardware

Particle operating pressure limit	275 bar (4,000 psi) for 10 µm particles 413 bar (6,000 psi) for 5 µm particles 551 bar (8,000 psi) for 3 µm particles 689 bar (10,000 psi) for 1.7 µm particles
Mobile phase compatibility	Compatible with aqueous solution buffers, acetonitrile/acetone/methanol and water mixtures. Commonly used buffers: phosphate, tris, MES and acetate
Working flow rate	Typical 0.1 - 1 mL/min for a 4.6 mm id column or 2.1 mm id column; always start a low flow rate and set default to the maximum hardware and/or particle pressure, whichever is lower.
Dynamic binding capacity		NP3	NP5	NP10
	SCX	53 mg/mL	38 mg/mL	20 mg/mL
	WCX	19 mg/mL	15 mg/mL	10 mg/mL
	SAX	35 mg/mL	28 mg/mL	17 mg/mL
	WAX	26 mg/mL	18 mg/mL	12 mg/mL

Literature

Agilent Bio HPLC Columns (26.00 MB)

Your essential resource for biomolecule analysis

Agilent Bio IEX Column Brochure (16.73 MB)

Now you have more columns and more choices to improve your charged-variant analysis of proteins with the Agilent Ion-exchange BioHPLC columns.

Agilent Bio IEX Columns Data Sheet (38.92 kB)

Data sheet for the Agilent Bio IEX Columns