Agilent Bio MAb
Agilent Bio MAb HPLC Columns
Thorough characterization of monoclonal antibodies includes the identification and monitoring of acidic and basic isoforms. The Agilent Bio MAb HPLC columns feature a unique resin specifically designed for high-resolution charge-based separations of monoclonal antibodies.
Bio MAb columns are available in 1.7 µm, 3 µm, 5 µm, and 10 µm sizes, providing higher resolution with the smaller particles. The Bio MAb column features a highly uniform, densely packed, weak cation exchange layer. This layer of the Bio MAb column is chemically bonded to the hydrophilic, polymeric coating of the Bio MAb column.
- A packing support composed of a rigid, spherical, highly cross-linked polystyrene divinylbenzene (PS/DVB) non-porous bead
- Particles grafted with a hydrophilic, polymeric layer, virtually eliminating non-specific binding of antibody proteins
- A different process is used to layer the weak cation-exchange phase to the particle making it a higher density than the Agilent Bio WCX column particles
- Specifically designed for the separation of charge isoforms of monoclonal antibodies
|Agilent Bio MAb|
|Bonded Phase||Weak Cation-Exchange (carboxylate)|
|ID||2.1 and 4.6 mm|
|Particle Size||1.7, 3, 5 and 10 µm|
|pH Stability||2 - 12|
|Operating Temperature Limit||80 ºC|
|Flow Rate||0.1 - 1 mL/min|