PL-SAX and PL-SCX

PL-SAX and PL-SCX Columns from Agilent

PL-SAX and PL-SCX columns are ideal for the ion-exchange HPLC separations of proteins and deprotected synthetic oligonucleotides under denaturing conditions.

PL-SAX has strong anion exchange functionality, covalently linked to a chemically stable polymer, extends the operating pH range. In addition, the anion exchange capacity is independent of pH. For synthetic oligonucleotides, separations using denaturing conditions of temperature, organic solvent, and high pH are all possible. PL-SAX delivers improved chromatography for self-complementary or G-rich sequences that may associate to form aggregates or hairpin structures. The 5 µm material provides high efficiency separations of n and n-1 sequences.

PL-SCX is a macroporous PS/DVB matrix with a very hydrophilic coating and strong cation-exchange functionality. This process is controlled to provide the optimum density of strong cation-exchange moieties for the analysis, separation and purification of a wide range of biomolecules, from small peptides to large proteins. Two pore sizes are available, 1000Å and 4000Å, to provide good mass transfer characteristics for a range of solute sizes. The 5 µm media delivers separations at higher resolution with the 30 µm media used for medium pressure liquid chromatography.

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Part Number	Short Description	Phase	Particle Size	ID	Length	Pore Size	Type	Price
PL1551-3102	PL-SAX 1000A 10um 150 x 4.6mm Column	PL-SAX	10 um	4.6 mm	150 mm	1000 A	Column	£663.00
PL1551-5102	PL-SAX 1000A 10um 250 x 4.6mm Column	PL-SAX	10 um	4.6 mm	250 mm	1000 A	Column	£786.00
PL1651-0102	PL-SAX 1000A 10um 5 x 3mm Guard Cartridge 2pk	PL-SAX	10 um	3.0 mm	5 mm	1000 A	Guard	£115.00
PL1551-3702	PL-SAX 1000A 30um 150 x 4.6mm Column	PL-SAX	30 um	4.6 mm	150 mm	1000 A	Column	£557.00
PL1551-5702	PL-SAX 1000A 30um 250 x 4.6mm Column	PL-SAX	30 um	4.6 mm	250 mm	1000 A	Column	£672.00
PL1951-1502	PL-SAX 1000A 5um 50 x 2.1mm Column	PL-SAX	5 um	2.1 mm	50 mm	1000 A	Column	£584.00
PL1551-1502	PL-SAX 1000A 5um 50 x 4.6mm Column	PL-SAX	5 um	4.6 mm	50 mm	1000 A	Column	£584.00
PL1551-1502PK	PL-SAX 1000A 5um 50 x 4.6mm PEEK Column	PL-SAX	5 um	4.6 mm	50 mm	1000 A	Peek Column	£584.00
PL1951-3802	PL-SAX 1000A 8um 150 x 2.1mm Column	PL-SAX	8 um	2.1 mm	150 mm	1000 A	Column	£698.00
PL1551-3802	PL-SAX 1000A 8um 150 x 4.6mm Column	PL-SAX	8 um	4.6 mm	150 mm	1000 A	Column	£698.00
PL1151-3802	PL-SAX 1000A 8um 150 x 7.5mm Column	PL-SAX	8 um	7.5 mm	150 mm	1000 A	Column	£1,138.00
PL1951-1802	PL-SAX 1000A 8um 50 x 2.1mm Column	PL-SAX	8 um	2.1 mm	50 mm	1000 A	Column	£539.00
PL1551-1802	PL-SAX 1000A 8um 50 x 4.6mm Column	PL-SAX	8 um	4.6 mm	50 mm	1000 A	Column	£539.00
PL1151-1802	PL-SAX 1000A 8um 50 x 7.5mm Column	PL-SAX	8 um	7.5 mm	50 mm	1000 A	Column	£868.00
PL1551-3103	PL-SAX 4000A 10um 150 x 4.6mm Column	PL-SAX	10 um	4.6 mm	150 mm	4000 A	Column	£663.00
PL1551-5103	PL-SAX 4000A 10um 250 x 4.6mm Column	PL-SAX	10 um	4.6 mm	250 mm	4000 A	Column	£786.00
PL1551-3703	PL-SAX 4000A 30um 150 x 4.6mm Column	PL-SAX	30 um	4.6 mm	150 mm	4000 A	Column	£557.00
PL1551-5703	PL-SAX 4000A 30um 250 x 4.6mm Column	PL-SAX	30 um	4.6 mm	250 mm	4000 A	Column	£672.00
PL1951-1503	PL-SAX 4000A 5um 50 x 2.1mm Column	PL-SAX	5 um	2.1 mm	50 mm	4000 A	Column	£584.00
PL1551-1503	PL-SAX 4000A 5um 50 x 4.6mm Column	PL-SAX	5 um	4.6 mm	50 mm	4000 A	Column	£584.00
PL1951-3803	PL-SAX 4000A 8um 150 x 2.1mm Column	PL-SAX	8 um	2.1 mm	150 mm	4000 A	Column	£698.00
PL1551-3803	PL-SAX 4000A 8um 150 x 4.6mm Column	PL-SAX	8 um	4.6 mm	150 mm	4000 A	Column	£698.00
PL1151-3803	PL-SAX 4000A 8um 150 x 7.5mm Column	PL-SAX	8 um	7.5 mm	150 mm	4000 A	Column	£1,138.00
PL1951-1803	PL-SAX 4000A 8um 50 x 2.1mm Column	PL-SAX	8 um	2.1 mm	50 mm	4000 A	Column	£539.00
PL1551-1803	PL-SAX 4000A 8um 50 x 4.6mm Column	PL-SAX	8 um	4.6 mm	50 mm	4000 A	Column	£539.00
PL1151-1803	PL-SAX 4000A 8um 50 x 7.5mm Column	PL-SAX	8 um	7.5 mm	50 mm	4000 A	Column	£868.00
PL1545-3102	PL-SCX 1000A 10um 150 x 4.6mm Column	PL-SCX	10 um	4.6 mm	150 mm	1000 A	Column	£663.00
PL1545-5102	PL-SCX 1000A 10um 250 x 4.6mm Column	PL-SCX	10 um	4.6 mm	250 mm	1000 A	Column	£786.00
PL1645-0102	PL-SCX 1000A 10um 5 x 3mm Guard Cartridge 2pk	PL-SCX	10 um	3.0 mm	5 mm	1000 A	Guard	£115.00
PL1545-3702	PL-SCX 1000A 30um 150 x 4.6mm Column	PL-SCX	30 um	4.6 mm	150 mm	1000 A	Column	£557.00
PL1545-5702	PL-SCX 1000A 30um 250 x 4.6mm Column	PL-SCX	30 um	4.6 mm	250 mm	1000 A	Column	£672.00
PL1945-1502	PL-SCX 1000A 5um 50 x 2.1mm Column	PL-SCX	5 um	2.1 mm	50 mm	1000 A	Column	£584.00
PL1545-1502	PL-SCX 1000A 5um 50 x 4.6mm Column	PL-SCX	5 um	4.6 mm	50 mm	1000 A	Column	£584.00
PL1945-3802	PL-SCX 1000A 8um 150 x 2.1mm Column	PL-SCX	8 um	2.1 mm	150 mm	1000 A	Column	£698.00
PL1545-3802	PL-SCX 1000A 8um 150 x 4.6mm Column	PL-SCX	8 um	4.6 mm	150 mm	1000 A	Column	£698.00
PL1945-1802	PL-SCX 1000A 8um 50 x 2.1mm Column	PL-SCX	8 um	2.1 mm	50 mm	1000 A	Column	£539.00
PL1545-1802	PL-SCX 1000A 8um 50 x 4.6mm Column	PL-SCX	8 um	4.6 mm	50 mm	1000 A	Column	£539.00
PL1145-1802	PL-SCX 1000A 8um 50 x 7.5mm Column	PL-SCX	8 um	7.5 mm	50 mm	1000 A	Column	£868.00

Features

PL-SAX

Small particles deliver excellent chromatographic performance
Wide range of particle sizes for flexible analysis and scale-up purification
Exceptional stability for long column lifetime

PL-SCX

Optimal design for effective separation of biomolecules
Pore sizes allow use of a range of solute sizes
Exceptional stability for long column lifetime

Specifications

	PL-SAX	PL-SCX
Bonded Phase	Strong Anion-Exchange	Strong Cation-Exchange
ID	2.1, 4.6, 7.5, 25, 50 and 100 mm
Particle Size	5, 8, 10 and 30 µm
Pore Size	1000 Å and 4000 Å
pH Stability	1 - 14
Operating Temperature Limit	80 ºC

Literature