
Zorbax Ion Exchange HPLC Columns from Agilent
ZORBAX Ion Exchange columns are available as both Strong Anion-Exchange (SAX) and Strong Cation-Exchange (300SCX) columns. Each column is packed with bonded, 5 µm, spherical silica particles for optimum efficiency in separating and purifying biological macromolecules, including proteins, peptides and polynucleotides.
ZORBAX Ion Exchange columns exhibit a high degree of mechanical stability. Therefore, they can be used at high operating flow rates and pressures. The columns are compatible with rapid changes in ionic strength, thus short gradient times and rapid re-equilibration are possible.
ZORBAX SAX packing has a permanently bonded quaternary amine. A trifunctional organo-silane reagent is used in producing this packing to maximize its stability with aqueous mobile phases. This column is ideal for separation of water-soluble compounds such as aromatic and aliphatic carboxylic acids and sulfonic acids.
ZORBAX SCX packing has 300 Å pore size silica particles chemically bonded to an aromatic sulfonic acid group. This column is used for separations of basic, water-soluble compounds and bio-molecules.
- ZORBAX SAX and 300SCX columns are based on rugged ZORBAX silica
- Stable from pH 2–7
- Provide high efficiency, rapid separations
- Compatible with organic mobile phase modifiers
ZORBAX SAX | ZORBAX 300SCX | |
Pore size | 70 Å | 300 Å |
Surface area | 300 m2/g | 50 m2/g |
pH range | 2 - 7 | 2 - 7 |
Functionality | Quaternary amine | Sulfonic acid |
USP designation | L14 | L9 |
Max pressure | 350 bar | 350 bar |