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Agilent Intact and Subunit Analysis Columns

For full characterization of a protein, such as a monoclonal antibody, it is necessary to look at the primary amino acid sequence and any post-translational modifications to the sequence that may have occurred during the purification or formulation steps of manufacture. To perform this type of analysis, denaturing conditions are required, so reversed-phase LC is normally the technique of choice.

Agilent offers a number of wide pore reversed-phase columns. From superficially porous, silica-based AdvanceBio RP-mAb to totally porous, polymer-based PLRP-S, there is a column solution for every intact or subunit protein separation.

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