Agilent Polaris Semi-Prep

Polaris SemiPrep/Preparative LC Columns from Agilent

Polaris columns have been designed for high aqueous conditions. The combination of high phase density bonding, ultra pure silica, and silanol shielding leads to excellent peak shape among polar-modified columns.

Polaris C18-A columns offer alternate selectivity for general polar applications and are designed with hydrogen-bond accepting endcapping.

Polaris C18-Ether columns are endcapped with an ether group to create a more polar surface for selectivity variation.

Polaris Amide-C18 columns provide subtle alternative selectivity because they do not have steric protection. They utilize an embedded amide, similar to Bonus-RP columns.

Polaris C8-A offers an alternative selectivity to standard C8 phases and has a lower hydrophobicity than Polaris C18-A, making it ideal for polar samples, for faster overall analysis times.

Polaris C8-Ether columns are endcapped with an ether group to create a more polar surface for selectivity variation.

Polaris Silica (Si) and Amino (NH₂) columns are ideal for retention of polar columns in normal phase mode.

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Part Number	Short Description	Phase Type	Phase	USP	Particle Size	ID	Length	Pore Size	Type	Price
A2002250X100	DG-Polaris 10 C18-A 250 x 10mm Column	C18 Modified	Polaris C18-A	L1	10 um	10.0 mm	250 mm	180 A	Column	£1,244.00
A2002250X212	DG-Polaris 10 C18-A 250 x 21.2mm Column	C18 Modified	Polaris C18-A	L1	10 um	21.2 mm	250 mm	180 A	Column	£2,440.00
A2002250X500	DG-Polaris 10 C18-A 250 x 50mm Column	C18 Modified	Polaris C18-A	L1	10 um	50.0 mm	250 mm	180 A	Column	£11,051.00
A2002050G500	DG-Polaris 10 C18-A 50 x 50mm Guard	C18 Modified	Polaris C18-A	L1	10 um	50.0 mm	50 mm	180 A	Guard	£4,619.00
A2004250X212	DG-Polaris 10 Si-A 250 x 21.2mm Column		Polaris Si-A		10 um	21.2 mm	250 mm	180 A	Column	£2,155.00
A2006100X212	DG-Polaris 5 Amide-C18 100 x 21.2mm Column	Polar Embedded	Polaris Amide-C18	L60	5 um	21.2 mm	100 mm	180 A	Column	£2,171.00
A2006250X100	DG-Polaris 5 Amide-C18 250 x 10mm Column	Polar Embedded	Polaris Amide-C18	L60	5 um	10.0 mm	250 mm	180 A	Column	£1,290.00
A2000100X212	DG-Polaris 5 C18-A 100 x 21.2mm Column	C18 Modified	Polaris C18-A	L1	5 um	21.2 mm	100 mm	180 A	Column	£2,171.00
A2000150X212	DG-Polaris 5 C18-A 150 x 21.2mm Column	C18 Modified	Polaris C18-A	L1	5 um	21.2 mm	150 mm	180 A	Column	£2,716.00
A2000250X100	DG-Polaris 5 C18-A 250 x 10mm Column	C18 Modified	Polaris C18-A	L1	5 um	10.0 mm	250 mm	180 A	Column	£1,460.00
A2000250X212	DG-Polaris 5 C18-A 250 x 21.2mm Column	C18 Modified	Polaris C18-A	L1	5 um	21.2 mm	250 mm	180 A	Column	£3,304.00
A2000250X500	DG-Polaris 5 C18-A 250 x 50mm Column	C18 Modified	Polaris C18-A	L1	5 um	50.0 mm	250 mm	180 A	Column	£16,894.00
A2000050G212	DG-Polaris 5 C18-A 50 x 21.2mm Guard	C18 Modified	Polaris C18-A	L1	5 um	21.2 mm	50 mm	180 A	Guard	£1,137.00
A2020250X100	DG-Polaris 5 C18-Ether 250 x 10mm Column	C18 Modified	Polaris C18-Ether	L1	5 um	10.0 mm	250 mm	180 A	Column	£1,290.00
A2020250X212	DG-Polaris 5 C18-Ether 250 x 21.2mm Column	C18 Modified	Polaris C18-Ether	L1	5 um	21.2 mm	250 mm	180 A	Column	£3,492.00
A2020050G212S	DG-Polaris 5 C18-Ether 50 x 21.2mm Guard	C18 Modified	Polaris C18-Ether	L1	5 um	21.2 mm	50 mm	200 A	Guard	£2,151.00
A2010250X100	DG-Polaris 5 C8-A 250 x 10mm Column	C8 Modified	Polaris C8-A	L7	5 um	10.0 mm	250 mm	180 A	Column	£1,460.00
A2010250X212	DG-Polaris 5 C8-A 250 x 21.2mm Column	C8 Modified	Polaris C8-A	L7	5 um	21.2 mm	250 mm	180 A	Column	£3,304.00
A2010050G212	DG-Polaris 5 C8-A 50 x 21.2mm Guard	C8 Modified	Polaris C8-A	L7	5 um	21.2 mm	50 mm	180 A	Guard	£1,434.00
A2030250X100	DG-Polaris 5 C8-Ether 250 x 10mm Column	C8 Modified	Polaris C8-Ether	L7	5 um	10.0 mm	250 mm	180 A	Column	£1,910.00
A2013250X100	DG-Polaris 5 NH2 250 x 10mm Column	Amino	Polaris NH2	L8	5 um	10.0 mm	250 mm	180 A	Column	£1,290.00
A2013250X212	DG-Polaris 5 NH2 250 x 21.2mm Column	Amino	Polaris NH2	L8	5 um	21.2 mm	250 mm	180 A	Column	£3,304.00
A2003250X212	DG-Polaris 5 Si-A 250 x 21.2mm Column		Polaris Si-A		5 um	21.2 mm	250 mm	180 A	Column	£3,304.00
A2003050X212	DG-Polaris 5 Si-A 50 x 21.2mm Column		Polaris Si-A		5 um	21.2 mm	50 mm	180 A	Column	£1,779.00
A2002050G100	Polaris 10 C18-A 50 x 10mm Guard	C18 Modified	Polaris C18-A	L1	10 um	10.0 mm	50 mm	180 A	Guard	£421.00
A2007050X100	Polaris 3 Amide-C18 50 x 10mm Column	Polar Embedded	Polaris Amide-C18	L60	3 um	10.0 mm	50 mm	180 A	Column	£773.00
A2021050X100	Polaris 3 C18-Ether 50 x 10mm Column	C18 Modified	Polaris C18-Ether	L1	3 um	10.0 mm	50 mm	180 A	Column	£614.00
A2006050G100	Polaris 5 Amide-C18 50 x 10mm Guard	Polar Embedded	Polaris Amide-C18	L60	5 um	10.0 mm	50 mm	180 A	Guard	£603.00
A2000100X100	Polaris 5 C18-A 100 x 10mm Column	C18 Modified	Polaris C18-A	L1	5 um	10.0 mm	100 mm	180 A	Column	£1,097.00
A2000030G212	Polaris 5 C18-A 120A 30 x 21.2mm Guard	C18 Modified	Polaris C18-A	L1	5 um	21.2 mm	30 mm	180 A	Guard	£733.00
A2000150X100	Polaris 5 C18-A 150 x 10mm Column	C18 Modified	Polaris C18-A	L1	5 um	10.0 mm	150 mm	180 A	Column	£1,169.00
A2000050G100	Polaris 5 C18-A 50 x 10mm Guard	C18 Modified	Polaris C18-A	L1	5 um	10.0 mm	50 mm	180 A	Guard	£502.00
A2020150X100	Polaris 5 C18-Ether 150 x 10mm Column	C18 Modified	Polaris C18-Ether	L1	5 um	10.0 mm	150 mm	180 A	Column	£1,096.00
A2020050G100	Polaris 5 C18-Ether 50 x 10mm Guard	C18 Modified	Polaris C18-Ether	L1	5 um	10.0 mm	50 mm	180 A	Guard	£589.00
A2010050G100	Polaris 5 C8-A 50 x 10mm Guard	C8 Modified	Polaris C8-A	L7	5 um	10.0 mm	50 mm	180 A	Guard	£429.00
A2030050G100	Polaris 5 C8-Ether 50 x 10mm Guard	C8 Modified	Polaris C8-Ether	L7	5 um	10.0 mm	50 mm	180 A	Guard	£1,258.00
A2013050G100	Polaris 5 NH2 50 x 10mm Guard	Amino	Polaris NH2	L8	5 um	10.0 mm	50 mm	180 A	Guard	£480.00
A2003150X100	Polaris 5 Si-A 150 x 10mm Column		Polaris Si-A		5 um	10.0 mm	150 mm	180 A	Column	£1,184.00
A2003050G100	Polaris 5 Si-A 50 x 10mm Guard		Polaris Si-A		5 um	10.0 mm	50 mm	180 A	Guard	£425.00

Features

Excellent chromatography with mixtures of polar and nonpolar analytes
Various unique selectivities
Resilient Polaris NH₂ phase offers rapid analysis of carbohydrates and extended lifetime compared to other NH₂ phases

Specifications

Polaris C18 Specifications

	Polaris C18-A	Polaris C18-Ether	Polaris Amide C18
Pore Size	180 Å	180 Å	180 Å
Surface Area	200 m²/g	200 m²/g	200 m²/g
Carbon Load	13.8%	12.1%	15%
Endcapped	Yes	Yes	Yes
Pore Volume	1.1 cm³/g	1.1 cm³/g	1.1 cm³/g
Ligand Coverage	3.9 µmol/m²	3.3 µmol/m²	4.4 µmol/m²
USP Designation	L1	L1	L60

Polaris C8 Specifications

	Polaris C8-A	Polaris C8-Ether
Pore Size	180 Å	180 Å
Surface Area	200 m²/g	200 m²/g
Carbon Load	7.4%	7.1%
Endcapped	Yes	Yes
Pore Volume	1.1 cm³/g	1.1 cm³/g
Ligand Coverage	4.8 µmol/m²	4.5 µmol/m²
USP Designation	L7	L7

Polaris Normal Phase Specifications

	Polaris NH₂	Polaris Si-A
Pore Size	180 Å	180 Å
Surface Area	200 m²/g	200 m²/g
Carbon Load	5.5%	-
Endcapped	Amide	-
Pore Volume	1.1 cm³/g	1.1 cm³/g
Ligand Coverage	3.8 µmol/m²
USP Designation	L8	L3

Literature

Prep Columns Brochure (1.73 MB)

Overview of Agilent's range of prep columns for small molecules and biomolecules

Agilent Polaris Columns Brochure (694.88 kB)

Confidently separate challenging polar compounds with a range of selectivity options