Protein Crash Plate

Protein Crash Plates from Porvair

Biological samples commonly contain proteins that interfere with downstream applications. The Microlute Protein Precipitation Plate uses the CRASH method, in which the protein is denatured with acetronitrile and the flocculant filtered out, allowing 96 samples to be handled at one time.

The Protein Precipitation Plate is based on the industry standard Microlute format, but without the chromatographic sorbent. Protein ‘crashes’ out of solution and precipitates directly in each well when acetonitrile is added, thus solving all common problems associated with the CRASH technique of protein clean-up.

The novel dual frit, hydrophobically treated matrix means that there is no ‘wetting out’ and leakage of the sample through the plate before the application of vacuum.

Protein Crash Plate

  • Dual frit design
  • Pre-filter frit at 100 µm traps large flocculant particles
  • Secondary frit traps fine protein particles at <10 µm
  • Frits are Hydrophobic/Oleophobic. This retains sample/acetonitrile in the well to allow precipitation of proteins until vacuum is applied.
  • Pore size optimised to allow ideal flow rate
  • Inert filter material to ensure no adsorption of sample components
  • Frit structure prevents break through of protein particles
  • Industry standard MicroLute format enables easy automation
  • Specially selected polypropylene for low extractables
  • The Protein MicroLute High Efficiency plate is exactly the same as the P3 but with the frits untreated. This plate is used when sample and acetronitrile are pre-mixed before being pipetted into the plate.