Thermo Scientific HyperSep Online SPE

Thermo Scientific HyperSep online SPE products retain specific analytes in a sample matrix when used with an appropriate HPLC column. They have been designed to clean-up and concentrate target analytes in minutes.

HyperSep online SPE products are available in three configurations:
- UNIGUARD Direct-Connect Online SPE Cartridges - convenient reusable product that attaches directly to the switching valve like a HPLC column
- Javelin Direct-Connect Online Columns - finger-tight design for convenience and maximum efficiency
- HPLC Columns for Online SPE - traditional HPLC column fittings and hardware

All products are available with the following HyperSep SPE chemistries:
- HyperSep Retain PEP - for retention of polar and non-polar analytes
- HyperSep Retain CX - for retention of basic and non-polar analytes
- HyperSep Retain AX - for retention of acidic and non-polar analytes
- HyperSep Hypercarb - for retention of extremely polar analytes

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Buy

Part Number	Short Description	SPE Format	Phase Type	Phase	Particle Size	Pore Size	Material	Pack Size	Price
60310-201	Retain PEP, 10 x 2.1mm, Javelin, 4pk	Javelin Online SPE Column	Reversed Phase	HyperSep Retain PEP	40 - 60 um	55 - 90 A	Polymeric	4	£300.18
60310-202	Retain PEP, 10 x 3mm, Javelin, 4pk	Javelin Online SPE Column	Reversed Phase	HyperSep Retain PEP	40 - 60 um	55 - 90 A	Polymeric	4	£296.68
60310-301	Retain CX, 10 x 2.1mm, Javelin, 4pk	Javelin Online SPE Column	SCX	HyperSep Retain CX	40 - 60 um	55 - 90 A	Polymeric	4	£300.18
60310-302	Retain CX, 10 x 3mm, Javelin, 4pk	Javelin Online SPE Column	SCX	HyperSep Retain CX	40 - 60 um	55 - 90 A	Polymeric	4	£300.18
60310-401	Retain AX, 10 x 2.1mm, Javelin, 4pk	Javelin Online SPE Column	SAX	HyperSep Retain AX	40 - 60 um	55 - 90 A	Polymeric	4	£300.18
60310-402	Retain AX, 10 x 3mm, Javelin, 4pk	Javelin Online SPE Column	SAX	HyperSep Retain AX	40 - 60 um	55 - 90 A	Polymeric	4	£300.18
60310-501	Hypercarb, 10 x 2.1mm, Javelin, 4pk	Javelin Online SPE Column	Carbon	HyperSep Hypercarb	30 um		Porous Graphitic Carbon	4	£322.37
60310-502	Hypercarb, 10 x 3mm, Javelin, 4pk	Javelin Online SPE Column	Carbon	HyperSep Hypercarb	30 um		Porous Graphitic Carbon	4	£309.31
60311-201	Retain PEP, 10 x 2.1mm, Drop-in Guard, 4pk	Online SPE Cartridge	Reversed Phase	HyperSep Retain PEP	40 - 60 um	55 - 90 A	Polymeric	4	£266.31
60311-202	Retain PEP, 10 x 3mm, Drop-in Guard, 4pk	Online SPE Cartridge	Reversed Phase	HyperSep Retain PEP	40 - 60 um	55 - 90 A	Polymeric	4	£270.98
60311-301	Retain CX, 10 x 2.1mm, Drop-In Guard, 4pk	Online SPE Cartridge	SCX	HyperSep Retain CX	40 - 60 um	55 - 90 A	Polymeric	4	£270.98
60311-302	Retain CX, 10 x 3mm, Drop-in Guard, 4pk	Online SPE Cartridge	SCX	HyperSep Retain CX	40 - 60 um	55 - 90 A	Polymeric	4	£270.98
60311-401	Retain AX, 10 x 2.1mm, Drop-in Guard, 4pk	Online SPE Cartridge	SAX	HyperSep Retain AX	40 - 60 um	55 - 90 A	Polymeric	4	£270.98
60311-402	Retain AX, 10 x 3mm, Drop-in Guard, 4pk	Online SPE Cartridge	SAX	HyperSep Retain AX	40 - 60 um	55 - 90 A	Polymeric	4	£270.98
60311-501	Hypercarb, 10 x 2.1mm, Drop-in Guard, 4pk	Online SPE Cartridge	Carbon	HyperSep Hypercarb	30 um		Porous Graphitic Carbon	4	£293.17
60311-502	Hypercarb, 10 x 3mm, Drop-in Guard, 4pk	Online SPE Cartridge	Carbon	HyperSep Hypercarb	30 um		Porous Graphitic Carbon	4	£293.17
60312-201	Retain PEP, 20 x 2.1mm, Online SPE column	Online SPE Column	Reversed Phase	HyperSep Retain PEP	40 - 60 um	55 - 90 A	Polymeric	1	£354.30
60312-202	Retain PEP, 20 x 3mm, Online SPE column	Online SPE Column	Reversed Phase	HyperSep Retain PEP	40 - 60 um	55 - 90 A	Polymeric	1	£374.93
60312-301	Retain CX, 20 x 2.1mm, Online SPE column	Online SPE Column	SCX	HyperSep Retain CX	40 - 60 um	55 - 90 A	Polymeric	1	£383.11
60312-302	Retain CX, 20 x 3mm, Online SPE column	Online SPE Column	SCX	HyperSep Retain CX	40 - 60 um	55 - 90 A	Polymeric	1	£374.93
60312-401	Retain AX, 20 x 2.1mm, Online SPE column	Online SPE Column	SAX	HyperSep Retain AX	40 - 60 um	55 - 90 A	Polymeric	1	£354.30
60312-402	Retain AX, 20 x 3mm, Online SPE column	Online SPE Column	SAX	HyperSep Retain AX	40 - 60 um	55 - 90 A	Polymeric	1	£374.93
60312-501	Hypercarb, 20 x 2.1mm, Online SPE Column	Online SPE Column	Carbon	HyperSep Hypercarb	30 um		Porous Graphitic Carbon	1	£381.30
60312-502	Hypercarb, 20 x 3mm, Online SPE Column	Online SPE Column	Carbon	HyperSep Hypercarb	30 um		Porous Graphitic Carbon	1	£402.97

Features

Available in several convenient formats for sample preparation and provide quick and easy connection
Choose from HyperSep Retain PEP, Retain CX, Retain AX, and Hypercarb phases
Use these online SPE columns with an appropriate HPLC column and switching valve
Effective removal of contaminants such as proteins from samples
Compatible with conventional HPLC systems
Fast and effective clean-up and concentration of target compounds

Specifications

	Retain PEP	Retain AX	Retain CX	Hypercarb
Phase	Polystyrene DVB material surface modified with urea groups	Polystyrene DVB material partially modified with quaternary amine	Polystyrene DVB material modified with sulfonic acid	100% porous graphitic carbon
Surface area	550 to 750 m²/g	550 to 750 m²/g	550 to 750 m²/g	-
Particle size	40 to 60 µm	40 to 60 µm	40 to 60 µm	30 µm
Pore size	55 to 90 Å	55 to 90 Å	55 to 90 Å	250 Å
pH stable	0 to 14	0 to 14	0 to 14	0 to 14

Literature

Thermo Sample Preparation Catalogue 2022-2023 (7.34 MB)

Save time, improve reproducibility, and extend the lifetime of your HPLC/UHPLC and GC columns with Thermo Scientific's comprehensive range of sample preparation products

Thermo Scientific SPE Selection Poster (258.34 kB)

Learn about Thermo Scientific's polymeric, reversed phase silica, normal phase silica, and ion-exchange phase options

HyperSep Applications Notebook (8.25 MB)

Brochure containing over 100 applications using HyperSep SPE products