Chromatography Tips

By Tony Taylor - Crawford Scientific's Technical Director

Tony has been doing, researching, teaching and training in analytical chemistry for the past 28 years. He comes from a pharmaceutical and polymer analysis background and continues to work with both liquid and gas phase techniques at Crawford Scientific (UK). His main interests are the use of LC-MS and GC-MS for structural characterisation and the quantitation of trace components in complex matrices. He is a professionally qualified trainer and the Technical Director of the CHROMacademy.  

Underutilised methods for optimising LC-MS sensitivity 
I hear the words ‘struggling for sensitivity’ so often when speaking to folks using LC-MS for bioanalysis, environmental analysis, metabolomics, proteomics and a host of other applications where target analytes are present at low concentrations in complex matrices.

Data Systems - do you know where your results comes from?
I’m sure most of us will be aware of the Able Labs fraud of the early 2000’s in which chromatographic data was manipulated to ensure that failing batches of generics drugs were in fact passed as pit for purpose [1,2]. This fraud involved manipulation of chromatographic baselines, as well as adjustments of weights, purity factors, and calculations to ensure acceptable results.

Going back in time – Ternary and Quaternary Gradients
Until recently, I hadn’t heard of ternary or quaternary gradients being used for many years. They have gained a reputation for being somewhat difficult to reproduce – less robust if you like.

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