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Thermo Scientific Acclaim 300 C18 Columns

Acclaim 300 C18 Biochromatography Columns from Thermo Scientific

Achieve high-resolution peptide mapping and protein separations with Thermo Scientific Acclaim 300 C18 LC Columns.

The 3 µm ultrapure silica particles reduce the limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 µm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns for faster separations.

Thermo Scientific Acclaim 300 C18 columns are designed for the separation of proteins, peptides, and other biological macromolecules. This line of columns extends the same unique bonding chemistry developed for the Acclaim 120 columns to a wide-pore silica substrate. The Acclaim 300 columns consist of 3 µm diameter silica particles with 300 Å diameter pores, bonded with ahighly characterized C18 phase. The unique bonding chemistry results in a high-density, highly uniform phase coverage with extensive end-capping. The use of a 3 µm silica particle accelerates the diffusion of the mobile phase into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 µm column packings, it is possible to increase the flow rate of the mobile phase and run shallower gradients on shorter columns to achieve the desired separation in a shorter time period.

Part Number Short Description Phase Type Phase USP Particle Size ID Length Pore Size Type Price Qty
060263 Acclaim 300 C18, 3um, 2.1 x 50mm C18 Acclaim C18 L1 3 um 2.1 mm 50 mm 300 A Column
£375.07
060264 Acclaim 300 C18, 3um, 2.1 x 150mm C18 Acclaim C18 L1 3 um 2.1 mm 150 mm 300 A Column
£568.40
060265 Acclaim 300 C18, 3um, 4.6 x 50mm C18 Acclaim C18 L1 3 um 4.6 mm 50 mm 300 A Column
£375.07
060266 Acclaim 300 C18, 3um, 4.6 x 150mm C18 Acclaim C18 L1 3 um 4.6 mm 150 mm 300 A Column
£568.40
063684 Acclaim 300 C18, 3um, 3 x 150mm C18 Acclaim C18 L1 3 um 3.0 mm 150 mm 300 A Column
£568.40
069690 Acclaim 300 C18, 3um, 2.1 x 10mm, Guard Cartridge, 2pk C18 Acclaim C18 L1 3 um 2.1 mm 10 mm 300 A Guard
£175.85
069697 Acclaim 300 C18, 3um, 4.6 x 10mm, Guard Cartridge, 2pk C18 Acclaim C18 L1 3 um 4.6 mm 10 mm 300 A Guard
£152.74
  • High-performance C18 bonding chemistry on 300 Å pore silica
  • Application tested for suitability in peptide mapping
  • Reproducible for dependable results
  • Extremely low bleed, fully compatible with MS
  • Stable between pH 2 .5 and 7.5
  Acclaim 300 C18
Bonded Phase C18
Particle Type Fully Porous
Particle Size 3 µm
Pore Size 300 Å
Surface Area 100 m2/g
Carbon Load 8%
Maximum Temperature 60°C
pH Range 2.5 - 7.5
USP Designation L1
Acclaim 300 C18 Specifications (232.64 kB)
Ideal for protein, peptide, and macromolecule separations
Analytical UHPLC and HPLC Columns Brochure (11.02 MB)
Introduction to the broad range of chemistries and column families from Thermo Scientific
LC Columns Technical Guide (13.60 MB)
Selection charts based on stationary phase use, analyte properties, LC-MS requirements or USP specifications
Thermo Scientific Bio LC Columns and Accessories Catalogue 2022-2023 (6.17 MB)
Thermo Scientific has innovative bio LC columns for each step of your therapeutic protein characterization, no matter how challenging your separation
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