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Thermo Scientific MAbPac SEC-1 Columns

Thermo Scientific MAbPac SEC-1 columns are designed for high resolution separation of monoclonal antibody (mAb) analysis, including the monomers, aggregates and Fab and Fc fragments resulting from proteolysis. These size exclusion columns provide superior performance, even under nondenaturing conditions, and in high and low salt mobile phases and volatile eluents.

Based on high purity, spherical porous 300Å 5µm silica particles that are covalently modified with a hydrophilic layer, this column offers separations with very low levels of secondary interaction. The stationary phase is designed to handle different eluent conditions containing both high and low ionic strength mobile phases, as well as mass spectrometry friendly volatile eluents.

Different column formats meet different analytical needs. The 4.0mm ID column enables baseline separation of mAb monomer and dimer. The 2.1mm ID column uses low flow rate and low sample loading, making it the perfect format for MS detection.

Part Number Short Description Phase USP Particle Size ID Length Pore Size Type Price Qty
088789 MAbPac SEC-1, 5um, 2.1 x 300mm, Column MAbPac SEC-1 L59 5 um 2.1 mm 300 mm 300 A Column
088790 MAbPac SEC-1, 5um, 2.1 x 150mm, Column MAbPac SEC-1 L59 5 um 2.1 mm 150 mm 300 A Column
074696 MAbPac SEC-1, 5um, 4 x 300mm, Column, 1pk MAbPac SEC-1 L59 5 um 4.0 mm 300 mm 300 A Column
074697 MAbPac SEC-1G, 5um, 4 x 50mm, Guard Column, 1pk MAbPac SEC-1 L59 5 um 4.0 mm 50 mm 300 A Guard
075592 MAbPac SEC-1, 5um, 4 x 150mm, Column, 1pk MAbPac SEC-1 L59 5 um 4.0 mm 150 mm 300 A Column
  • Hydrophilic bonded layer minimises undesired interactions between the biomolecules and the stationary phase
  • 2.1mm I.D. columns are packed into stainless steel housing
  • 4mm I.D. columns are packed into PEEK housing
  • Non-metallic and biocompatible PEEK housing for 4mm ID columns eliminates metal contamination from the column hardware
  • Stable surface bonding leads to low column bleed and compatibility with MS, ELSD and CAD detection
  • Rugged column packing provides reliability, reproducibility and long column life
  • Columns are available in 5µm particle size and 150 and 300mm lengths, with an average pore diameter of 300Å
  • Separation range for globular proteins is 1 – 1,000 kDa
  • USP designation of L59
  MAbPac SEC-1
Phase Proprietary Diol
Particle Type Fully Porous Silica
Particle Size 5 µm
Pore Size 300 Å
Molecular Weight Lower Limit 1000 Da
Molecular Weight Upper Limit 1,000,000 Da
pH Range 2.5 − 7.5
Maximum Temperature 30°C
Hardware PEEK for 4.0mm ID columns
Stainless Steel for 2.1mm ID columns
USP Designation L59
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