HPLC Method Development Incorporating Quality by Design
1 Day Course
- We recommend that course numbers are limited to 10 so that each delegate gets the opportunity to ask questions and fully participate in tutorial exercises
- As the training is delivered on-site, we can design the course material to suit your specific needs
- Customisable written assessments are available if required
Course Overview | Download course details »
This one day course provides a step-by step approach to method development for HPLC using Quality by Design (QBD) principles.
All sections are accompanied by participative tutorial exercises using interactive chromatgraphic modelling software simulations for increased understanding of critical concepts.
Who is this course for
This course is designed for the more experienced chromatographer with a good working knowledge of HPLC separations. Attendance of the Fundamental HPLC and HPLC Troubleshooting and Maintenance training courses is not compulsory but is advantageous.
Previous knowledge
Delegates should have a good knowledge of chromatography and experience as HPLC users. Some experience in method development is recommended. A good grounding in chemistry is also beneficial.
What you will learn
- Setting method development objectives
- Sample preparation
- System choices
- Choosing a column and mobile phase
- Optimisation strategies
- Quantitation and system characterisation
Course Outline
Introduction to QBD approach to method development
Combined resolution equation (k', N and alpha) with reference to practical method development process
Critical method parameters
- Selectivity
- Method robustness
Troubleshooting common problems relating to the sample
Mobile phase considerations
- Ionisation, pH and pKa
- Buffers - role, types and molarity
- Flowrates
- Organic modifier
Choosing a Column
- Physical dimensions (length, ID, pore size)
- Particle size, particle type, UHPLC
- Temperature
- Endcapping
- Bonded silica phases - pH and mobile phase restrictions
- Reversed phase ligand chemistry
- Column classification tests (Tanaka, PQRI, HSM etc.)
HILIC
Specificity, peak purity and detection
Gradient and isocratic elution
Prelimanary retention time scouting
Troubleshooting retention time issues
Second stage optimisation - optimising selectivity choices
Linearity, range and sensitivity
Methods of quantitative calibration
Pre-validation