J’sphere ODS-H80 is the most hydrophobic stationary phase in this series. It is densely covered with polymeric bonded C18 groups yielding a high carbon content and providing a strong, dominant, lipophilic interaction with the nonpolar sites of the sample molecules. However, the ability to form H-bonding gives additional selectivity, which is essential for difficult separations, such as drug and corresponding metabolite discrimination. Even stereoisomers can be separated by J’sphere ODS-H80 columns.