SiELC Obelisc R HPLC Columns
All pictures shown are for illustration purpose only. Actual product may vary.
SiELC Obelisc R is a mixed-mode column based on LiSC (Liquid Separation Cell technology) and is designed for use in traditional reversed-phase applications. LiSC technology is based on a chemical modification of the silica gel pores that creates a liquid separation cell with its own charge characteristics, ionic strength, and hydrophobic properties.
Obelisc R has a stationary phase with cationic groups close to the silica surface separated from anionic groups by a hydrophobic chain. Due to the presence of ionic groups and a long hydrophobic chain, Obelisc R offers additional retention and tuning that is not available with traditional reversed-phase columns. Typical mobile phases used are based acetonitrile, water, and the mass spectrometry compatible buffers ammonium formate (pH 3) and ammonium acetate (pH 5).
Features
- Due to the presence of ionic groups and a long hydrophobic chain, Obelisc R provides additional retention and tuning that traditional reversed-phase columns cannot offer
- Multiple separation modes available including reversed phase, normal phase, and ion exclusion
- Compatible with LC-MS, ELSD, and low UV (<220 nm) with low concentration buffers
- Adjustable selectivity in all modes based on organic content, pH, and buffer strength
- Obelisc range also includes Obelisc N with normal phase characteristics
- Scalable from capillary to prep
- Guard columns connect directly to columns and do not require any additional holders
Specification
| Obelisc R | |
| Mode | LiSC (Liquid Seperation Cell technology) |
| Phase Type | Mixed-mode |
| Particle Type | Fully Porous |
| Particle Size | 5 µm, 10 µm |
| Pore Size | 100 Å |
| Temperature Range | 0 - 50°C |
| pH Limit | 1.5 - 7.0 |
| Recommended pH Operating Range | 2.0 - 6.5 |
| Pressure Limit | 345 bar |
