Understanding HPLC
1 Day
This course introduces the fundamentally important concepts associated with High Performance Liquid Chromatography (HPLC) analysis
This course introduces the fundamentally important concepts associated with High Performance Liquid Chromatography (HPLC) analysis including hardware basics, modes of analysis, basic troubleshooting, column chemistry, important parameters of the mobile phase such as amount and type of organic solvents, pH, buffer strength and type, and much more.
With a strong focus on information relevant for day to day practical work, this training improves interest and confidence in HPLC, giving delegates greater self-sufficiency through error reduction and understanding of the entire HPLC process.
- Principles of chromatography and how this relates to HPLC
- Understanding of the chromatographic parameters
- Hardware overview – the function of each HPLC module
- The essential principles of mobile phases, buffers and eluent additives for HPLC
- HPLC column parameters and common stationary phases
The Chromatographic Process
- Overview of the reversed phase HPLC process
- Revision of polarity, solubility and diluents
- Introduction to chromatographic parameters, Capacity factor, Selectivity, Efficiency and Resolution
HPLC Mobile Phases and Additives
- Mobile phase preparation
- Organic modifiers
- Acids, bases pH control and buffering
- Ion pairing agents
HPLC Columns
- Column dimensions
- Silica based supports
- Bonded phases
- The van Deemter Equation
- Common reversed phase column chemistries
Introduction to Instrumentation
- The Pump
- The Autosampler
- Detectors – UV, RID, F, ELSD, CAD, ECD
Introduction to Other Modes of HPLC
- Reversed Phase
- Ion suppression and ion pair in RP
- Normal phase
- HILIC
- Size Exclusion
- Ion exchange
Data processing
- Peak integration
- Standards and calibration
- System suitability
Designed for delegates who have some practical experience working with HPLC, this training seeks to give a much wider and deeper understanding of the entire HPLC process.
Previous experience is not essential however delegates would benefit from a basic understanding of HPLC and some previous practical experience of HPLC.
HPLC Operator
HPLC Troubleshooting
HPLC Method Development
Calibration and Quantitation