Agilent Bio SEC Columns
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Agilent Bio SEC HPLC Columns
Agilent Bio SEC offer fast, reliable, accurate performance for your biopharmaceutical analysis. They are easy to integrate into your workflow, and are available in a full range of pore sizes and dimensions to ensure the perfect separation every time.
Agilent Bio SEC-3
These columns are a breakthrough technology for size exclusion chromatography (SEC). They are packed with spherical, narrowly dispersed 3 µm silica particles coated with a proprietary hydrophilic layer. This thin polymeric layer is chemically bonded to pure, mechanically stable silica under controlled conditions, ensuring a highly efficient size exclusion particle.
Available in 100Å, 150Å and 300Å pore sizes to accommodate most peptide and protein size exclusion separations.
Agilent Bio SEC-5
These columns are packed with 5 µm silica particles coated with a proprietary, neutral, hydrophilic layer for maximum efficiency and stability. Our specially designed packing also promotes high pore volume, improving both peak capacity and resolution.
Available in 100Å, 150Å, 300Å, 500Å, 1000Å and 2000Å.
Agilent Bio SEC columns offer fast, reliable, accurate performance for your biopharmaceutical analysis. They are easy to integrate into your workflow and are available in a full range of pore sizes and dimensions to ensure the perfect separation every time.
Bio SEC-3 HPLC bio columns are a breakthrough technology for size exclusion chromatography. These bio columns are packed with spherical, narrowly dispersed 3 μm silica particles coated with a proprietary hydrophilic layer. This thin polymeric layer is chemically bonded to pure, mechanically stable silica under controlled conditions, ensuring an efficient size exclusion particle. Bio SEC-3 HPLC columns are available in 100Å, 150Å and 300Å pore sizes to accommodate most peptide and protein size exclusion separations.
Bio SEC-5 HPLC size exclusion chromatography columns are packed with 5 μm silica particles coated with a proprietary, neutral, hydrophilic layer for maximum efficiency and stability. Specially designed packing also promotes high pore volume, improving both peak capacity and resolution. Bio SEC-5 HPLC columns are available in 100 Å, 150 Å, 300 Å, 500 Å, 1000 Å, and 2000 Å.
Features
- Exceptional loading capacity, stability, and reproducibility for size-based biomolecule separations
- Sharper peaks, higher resolution, and better protein recovery
- Faster separations than large-particle SEC columns
- Compatibility with most aqueous buffers
- Excellent stability in high-salt and low-salt conditions
- Wide range of pore size options, including 1000 Å and 2000 Å gives options for everything from peptides to VLPs
- Analytical and semi-prep dimensions for easy scale up or down
Specification
| Bio SEC-3 | Bio SEC-5 | ||||||||
| Pore Size | 100 Å | 150 Å | 300 Å | 100 Å | 150 Å | 300 Å | 500 Å | 1000 Å | 2000 Å |
| Particle Size | 3 µm | 3 µm | 3 µm | 5 µm | 5 µm | 5 µm | 5 µm | 5 µm | 5 µm |
| Particle Type | Fully Porous | Fully Porous | Fully Porous | Fully Porous | Fully Porous | Fully Porous | Fully Porous | Fully Porous | Fully Porous |
| Molecular Weight Lower Limit | 100 Da | 500 Da | 5000 Da | 100 Da | 500 Da | 5000 Da | 15,000 Da | 50,000 Da | |
| Molecular Weight Upper Limit | 100,000 Da | 150,000 Da | 1,250,000 Da | 100,000 Da | 150,000 Da | 1,250,000 Da | 5,000,000 Da | 7,500,000 Da | >10,000,000 Da |
| Temperature Limit | 80°C | 80°C | 80°C | 80°C | 80°C | 80°C | 80°C | 80°C | 80°C |
| pH range | 2 - 8.5 | 2 - 8.5 | 2 - 8.5 | 2 - 8.5 | 2 - 8.5 | 2 - 8.5 | 2 - 8.5 | 2 - 8.5 | 2 - 8.5 |
