YMC-Pack Diol SEC Columns
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YMC-Pack Diol SEC columns are available in four tightly controlled porosities and are suitable for separation and molecular weight determination of a wide range of peptides, proteins, oligonucleotides, carbohydrates and other biopolymers with molecular weights of approx 200 to several hundred thousand kDa.
YMC-Pack Diol stationary phases are surface modified for a versatile alternative to underivatised silica. Advantages include a shorter equilibration time as well as good selectivity. Due to this short equilibration time, the Diol phase is a perfect choice for SEC in comparison with underivatised silica.
Features
- Suitable for proteins, antibodies, their fragments and peptides
- Also applicable to carbohydrates and nucleic acid components
- Excellent reproducibility with minimal secondary interactions
- 2 µm particle size for UHPLC methods
Specification
| YMC-Pack Diol-60 | YMC-Pack Diol-120 | YMC-Pack Diol-200 | YMC-Pack Diol-300 | |
| Phase | Dihydroxypropyl | Dihydroxypropyl | Dihydroxypropyl | Dihydroxypropyl |
| Suitable for | carbohydrates & peptides | intermediate proteins | large proteins | very large proteins |
| Pore Size | 6 nm / 60 Å | 12 nm / 120 Å | 20 nm / 200 Å | 30 nm / 300 Å |
| Particle Size | 3, 5 µm | 3, 5 µm | 2, 3, 5 µm | 2, 3, 5 µm |
| Particle Type | Fully Porous | Fully Porous | Fully Porous | Fully Porous |
| Molecular Weight Lower Limit | 5 kDa | 5 kDa | 20 kDa | |
| Molecular Weight Upper Limit | <10 kDa | 100 kDa | 300 kDa | 1000 kDa |
| pH Range | 5 - 7.5 | 5 - 7.5 | 5 - 7.5 | 5 - 7.5 |
| Maximum Temperature | 40°C | 40°C | 40°C | 40°C |
| USP Designation | L20 | L20 | L20 | L20 |
Literature
Specifications, target molecules and typical applications of YMC resins for biomolecule purification
Full details on the YMC classic range of HPLC columns
Recommended YMC columns for oligonucleotide separations
Overview of YMC's range of columns for biochromatography
Stationary phases for Bio LC from YMC