HILIC HPLC Columns

Hydrophilic interaction liquid chromatography (HILIC) is a powerful technique for separating polar and highly polar compounds that are poorly retained by reversed phase methods. HILIC columns create a water-enriched layer onto the stationary phase surface, enhancing interactions with polar analytes for improved retention and resolution. This makes HILIC chromatography particularly valuable for challenging applications, including metabolites, sugars, nucleotides, and other highly hydrophilic compounds. 

Our portfolio of HILIC columns includes a variety of stationary phases to suit different selectivity needs. Options range from traditional bare silica to bonded phases such as diol, amino, and zwitterionic, as well as HILIC amide columns offering excellent peak shape, short equilibration times, and high reproducibility. Whether you require a standard HILIC column for routine polar compound analysis or a specialised HILIC UHPLC solution for complex workflows, our selection is designed to deliver reliable, high-performance results across diverse applications.