SiELC Primesep B4 HPLC Columns
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SiELC Primesep B4 is a short carbon chain reversed phase mixed-mode HPLC column with embedded basic ion-pairing groups. It provides enhanced retention of acidic compounds through an anion-exchange mechanism, retains basic and acidic surfactants using a multi-mode mechanism, separates hydrophobic bases via an ion-exclusion mechanism, and retains hydrophobic neutral compounds through a reverse-phase mechanism. The short carbon chain in the Primesep B4 stationary phase allows hydrophobic compounds to elute in a shorter time compared to phases with longer chain.
Features
- Primesep B4 stationary phase has basic ion-pairing groups embedded within a short carbon chain
- Enhanced retention of acidic compounds through an anion-exchange mechanism
- Retains and separates basic and acidic surfactants using a multi-mode mechanism
- Separates hydrophobic bases via an ion-exclusion mechanism
- Retains hydrophobic neutral compounds through a reverse-phase mechanism
- Offers mobile phase compatibility with LC-MS
- Resistant to dewetting in 100% aqueous mobile phase
- Scalable from capillary to prep
- Guard columns connect directly to columns and do not require any additional holders
Specification
| Primesep B4 | |
| Mode | Mixed-Mode |
| Phase Type | Basic ion-pairing groups embedded in short carbon chains |
| Particle Type | Fully Porous |
| Particle Size | 5 µm |
| Pore Size | 100 Å |
| Temperature Range | 0 - 50°C |
| pH Limit | 1.5 - 5.0 |
| Recommended pH Operating Range | 2.0 - 4.5 |
| Pressure Limit | 345 bar |
Literature
Creating new dimensions in the world of chromatography
Solving problems of pharmaceutical HPLC analysis using Primesep columns
New alternative to ion chromatography