Zetarose Tag Affinity Bulk Resin from emp BIOTECH
All pictures shown are for illustration purpose only. Actual product may vary.
Zetarose Tag Affinity bulk resin from emp BIOTECH is a robust affinity chromatography resin designed for the purification of polyhistidine-tagged proteins using immobilised-metal affinity chromatography (IMAC). The highly cross-linked 6% agarose matrix is derivatised with nitrilotriacetic acid (NTA), which, when charged with bivalent metal ions, selectively binds His-tagged proteins through their affinity to the metal ion coordination sphere. Proteins bound to the resin can be eluted under native or denaturing conditions using low pH buffers or buffers containing imidazole or histidine. The choice of metal cation can be tailored to specific applications to optimise binding affinity and specificity for the target His-tagged proteins. Zetarose NTA FF6 offers repeated purification cycles, providing a cost-effective solution for high-end affinity separations.
Features
- Immobilised-metal affinity chromatography (IMAC) solid phases for the purification of polyhistidine-tagged proteins
- Designed to maximise the purification of polyhistidine-tagged proteins with minimal nonspecific binding
- High cross-linking 6% agarose matrix provides enhanced stability and reusability for repeated purification cycles
- Derivatised with nitrilotriacetic acid (NTA) for selective binding of His-tagged proteins
- Supports bivalent metal ions (e.g., Ni2+, Co2+, Cu2+) to tailor binding affinity and specificity
- Proteins can be eluted under native or denaturing conditions using low pH buffers or buffers containing imidazole or histidine
- Engineered for repeated use, offering a cost-effective solution for high-end affinity separations
- Also available as ZetaSep tag affinity FPLC columns
Specification
| Zetarose NTA (metal-free) | Zetarose Ni-NTA | |
| Binding Capacity | – | ≥40 mg His-tagged protein*/mL resin |
| Ligand Density | ≥20 μmol Ni2+/mL | ≥20 μmol Ni2+/mL |
| Velocity Properties | ≥500 cm/h, ≤3 bar | ≥500 cm/h, ≤3 bar |
| His-tag Affinity | +++ | |
| His-tag Specificity | ++ |
* SBC (HNRNPDL-protein, MW ~45-50 kDa)
