HANDS-ON LC-MS TROUBLESHOOTING & MAINTENANCE

Practical in-person training delivered by Crawford Scientific's highly experienced training team exclusively at the National Brownfield Institute, Wolverhampton.

LC-MS is not just another detector; it is a complex and often unforgiving technique. This complexity extends to troubleshooting, where many issues are unique to the technique and require specific approaches to identify and resolve. In this course we build the strong foundational understanding of LC-MS, how common issues arise, and the specific techniques we apply to identify and resolve issues. Written and delivered by experienced LC-MS specialists, we provide you with practical approaches to all aspects of troubleshooting LC-MS - whether it’s poor sensitivity, inconsistent results, or unknown contamination - knowing where and how to troubleshoot is essential for anyone routinely operating LC-MS.

LC-MS Troubleshooting is a solutions-focused course designed to help analysts and scientists systematically identify, understand, and resolve common and complex issues in LC-MS workflows. This course will empower you to take control of your instrument's performance, build the confidence to operate this technique routinely and achieve optimal productivity from this valuable technique.

You’ve made the substantial capital investment in purchasing your LC-MS hardware, let Crawford Scientific allow you to maximise productivity from your LC-MS by minimising downtime, reducing reliance on vendor support, and achieving better reliability. LC-MS Troubleshooting delivers real-world knowledge that saves time, conserves resources, and enhances analytical accuracy across your lab.

Date: 15th - 16th September

Time: 9.30am – 4.30pm

Duration: 2 days

Number of delegates: 8

Location: National Brownfield Institute, Wolverhampton, WV10 0JR

Price: £1,198 (+VAT) per person

Enquire Now

Hands-On Courses

PRACTICAL LC-MS TROUBLESHOOTING AND MAINTENANCE COURSE DETAILS

Key solution chemistry factors: polarity, pH, buffers, and additives
Ionisation techniques (ESI, APCI) and sensitivity optimisation
Fragmentation and ion focussing basics
Vacuum system impact on performance
Identifying and managing ion suppression/enhancement
Contamination prevention and cleaning strategies
Tuning vs. calibration and how to interpret reports
Compound-specific optimisation techniques
Distinguishing LC vs. MS-related issues
Benchmarking system performance
Troubleshooting sensitivity and precision problems
A step-by-step troubleshooting process
Hands-on troubleshooting with real-world case studies

This course is designed for those relatively new to LC-MS.

Solution Chemistry
- Positive and negative ion acquisition
- Molecule polarity
- pH and pKa
- Buffers
- Mobile phase additives
Ionisation
- Electrospray
- Atmospheric Pressure Chemical Ionisation
- Factors affecting sensitivity
Instrumentation
- Collision Induced dissociation
Vacuum System
Ionisation Suppression and Enhancement
- Ion suppression
- Specificity and selectivity
Contamination and Cleaning
Tuning and Calibration
- What’s the difference?
- Profile tuning
- Autotune report interpretation
Compund Specific Optimisation
LC or MS?
- Potential sources of common issues
Benchmarking Performance
Troubleshooting Sensitivity Issues