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Agilent AdvanceBio SEC Columns

AdvanceBio SEC HPLC Columns from Agilent

AdvanceBio SEC columns deliver accurate, precise quantitation for mAb aggregation and protein analysis. These innovative size exclusion chromatography columns were designed and engineered by Agilent to improve lab productivity by providing robust, reliable methods that eliminate sample reanalysis. Consistent results are achieved from column to column, batch to batch, and lab to lab, ensuring that methods can be transferred across departments and locations to put an end to uncertainty.

The AdvanceBio SEC 1.9 µm column is designed to improve resolution and provided faster separation of mAb aggregates and fragments by using both HPLC and UHPLC. The optimized sub-two-micron particles are monodispersed and modified by unique hydrophilic bonding chemistry providing column reproducibility and stability, as well as preventing secondary interactions and protein adsorption. The AdvanceBio SEC 1.9 µm column is intended to meet the need for qualitative and quantitative critical quality attributes (CQA) analysis and method transferability across departments.

Agilent AdvanceBio SEC columns ensure easy, fast, and accurate quantitation for mAb aggregation and fragment analysis. AdvanceBio SEC offers two different particle sizes, 2.7 and 1.9 µm. These innovative size exclusion chromatography (SEC) columns were designed and engineered by Agilent to achieve high resolution and efficiency separation, with a robust and reliable performance. Consistent results are obtained from column to column, batch-to-batch, and lab to lab, ensuring that methods can be transferred across departments and locations to put an end to uncertainty.

The AdvanceBio SEC 1.9 µm columns are designed to improve resolution and provided faster separation of protein aggregates and fragments by UHPLC. The optimized sub 2 µm particles are monodispersed and modified by unique hydrophilic bonding chemistry providing column reproducibility, stability as well as preventing secondary interactions and protein adsorption. The AdvanceBio SEC 1.9 μm column is intended to meet the needs for qualitative and quantitative critical quality attributes (CQA) analysis and method transferability across departments.

Part Number Short Description Phase Particle Size ID Length Pore Size Type Price Qty
PL1980-3250PK AdvanceBio SEC 120A 2.1 x 150mm 1.9um PEEK AdvanceBio SEC 1.9 um 2.1 mm 150 mm 120 A PEEK Column
£1,438.00
PL1980-1250PK AdvanceBio SEC 120A 2.1 x 50mm 1.9um PEEK AdvanceBio SEC 1.9 um 2.1 mm 50 mm 120 A PEEK Column
£1,329.00
PL1580-3250 AdvanceBio SEC 120A 4.6 x 150mm 1.9um AdvanceBio SEC 1.9 um 4.6 mm 150 mm 120 A Column
£1,858.00
PL1580-3250K AdvanceBio SEC 120A 4.6 x 150mm 1.9um Method Validation Kit AdvanceBio SEC 1.9 um 4.6 mm 150 mm 120 A Validation Kit
£5,018.00
PL1580-1250 AdvanceBio SEC 120A 4.6 x 30 mm 1.9um Guard Column AdvanceBio SEC 1.9 um 4.6 mm 30 mm 120 A Guard
£564.00
PL1580-5250 AdvanceBio SEC 120A 4.6 x 300mm 1.9um AdvanceBio SEC 1.9 um 4.6 mm 300 mm 120 A Column
£2,642.00
PL1580-5250K AdvanceBio SEC 120A 4.6 x 300mm 1.9um Method Validation Kit AdvanceBio SEC 1.9 um 4.6 mm 300 mm 120 A Validation Kit
£6,882.00
PL1580-3350 AdvanceBio SEC 130A 4.6 x 150mm 2.7um AdvanceBio SEC 2.7 um 4.6 mm 150 mm 130 A Column
£1,040.00
PL1580-5350 AdvanceBio SEC 130A 4.6 x 300mm 2.7um AdvanceBio SEC 2.7 um 4.6 mm 300 mm 130 A Column
£1,433.00
PL1580-1350 AdvanceBio SEC 130A 4.6 x 50mm 2.7um Guard AdvanceBio SEC 2.7 um 4.6 mm 50 mm 130 A Guard
£532.00
PL1180-3350 AdvanceBio SEC 130A 7.8 x 150mm 2.7um AdvanceBio SEC 2.7 um 7.8 mm 150 mm 130 A Column
£1,186.00
PL1180-5350 AdvanceBio SEC 130A 7.8 x 300mm 2.7um AdvanceBio SEC 2.7 um 7.8 mm 300 mm 130 A Column
£1,636.00
PL1180-1350 AdvanceBio SEC 130A 7.8 x 50mm 2.7um Guard AdvanceBio SEC 2.7 um 7.8 mm 50 mm 130 A Guard
£532.00
PL1980-3201PK AdvanceBio SEC 200A 2.1 x 150mm 1.9um PEEK AdvanceBio SEC 1.9 um 2.1 mm 150 mm 200 A PEEK Column
£1,438.00
PL1980-1201PK AdvanceBio SEC 200A 2.1 x 50mm 1.9um PEEK AdvanceBio SEC 1.9 um 2.1 mm 50 mm 200 A PEEK Column
£1,329.00
PL1580-3201 AdvanceBio SEC 200A 4.6 x 150mm 1.9um AdvanceBio SEC 1.9 um 4.6 mm 150 mm 200 A Column
£1,858.00
PL1580-5201 AdvanceBio SEC 200A 4.6 x 300mm 1.9um AdvanceBio SEC 1.9 um 4.6 mm 300 mm 200 A Column
£2,642.00
PL1580-1201 AdvanceBio SEC 200A 4.6 x 30mm 1.9um Guard AdvanceBio SEC 1.9 um 4.6 mm 30 mm 200 A Guard
£564.00
PL1580-3301 AdvanceBio SEC 300A 4.6 x 150mm 2.7um AdvanceBio SEC 2.7 um 4.6 mm 150 mm 300 A Column
£1,040.00
PL1580-5301 AdvanceBio SEC 300A 4.6 x 300mm 2.7um AdvanceBio SEC 2.7 um 4.6 mm 300 mm 300 A Column
£1,433.00
PL1580-1301 AdvanceBio SEC 300A 4.6 x 50mm 2.7um Guard AdvanceBio SEC 2.7 um 4.6 mm 50 mm 300 A Guard
£532.00
PL1180-3301 AdvanceBio SEC 300A 7.8 x 150mm 2.7um AdvanceBio SEC 2.7 um 7.8 mm 150 mm 300 A Column
£1,186.00
PL1180-5301 AdvanceBio SEC 300A 7.8 x 300mm 2.7um AdvanceBio SEC 2.7 um 7.8 mm 300 mm 300 A Column
£1,636.00
PL1180-1301 AdvanceBio SEC 300A 7.8 x 50mm 2.7um Guard AdvanceBio SEC 2.7 um 7.8 mm 50 mm 300 A Guard
£532.00
  • High resolution, high-efficiency separation, and accurate quantitation for aggregates and fragments analysis
  • Prevented secondary interactions with mAb, ADC, and other proteins
  • Assured consistent column performance to eliminate rework
  • Enhanced column lifetime and productivity to meet robust CQA analysis
  • Specially designed standards for AdvanceBio SEC columns, providing optimal calibration and performance verification
  • AdvanceBio SEC 300 Å 2.7 µm is intended for separation protein ranging from 5,000 to 1.25 million Da including mAb fragments, mAb monomer, dimer, and high-level aggregates
  • AdvanceBio SEC 130 Å 2.7 µm is designed for small therapeutics molecular weight ranging from 100 to 120,000 Da
  • AdvanceBio SEC 200 Å 1.9 µm is suited for analyzing protein ranging from 2,000 to 700,000 Da including mAb monomer, dimmer, and LMW protein fragments
  • AdvanceBio SEC 120 Å 1.9 µm is best suited for analysis of peptides and small therapeutic proteins in the range 100 to 80,000 Da
  AdvanceBio SEC
Particle size 1.9 µm 1.9 µm 2.7 µm 2.7 µm
Pore Size 120 Å 200 Å 130 Å 300 Å
Particle Type Fully Porous Fully Porous Fully Porous Fully Porous
Molecular Weight Lower Limit 1000 Da 2000 Da 100 Da 5000 Da
Molecular Weight Upper Limit 80,000 Da 700,000 Da 120,000 Da 1,250,000 Da
Temperature Limit 80°C 80°C 80°C 80°C
pH range 2 - 8.5 2 - 8.5 2 - 8.5 2 - 8.5
 

 

AdvanceBio 2.7 µm SEC Brochure (1.68 MB)
End uncertainty in monoclonal antibody characterization with AdvanceBio 2.7 µm columns
AdvanceBio SEC 120A 1.9um Brochure (1.60 MB)
High-resolution analysis of peptides and proteins with AdvanceBio SEC 120 Å 1.9 µm columns
AdvanceBio SEC 200A 1.9um Brochure (1.68 MB)
Fast separations for aggregates and fragments with AdvanceBio SEC 200 Å 1.9 µm columns
Aggregate Analysis Application Compendium (6.82 MB)
Collection of Agilent app notes on aggregate/fragment analysis
mAb Derived Therapeutics using Native SEC Workflow Ordering Guide (230.20 kB)
Workflow ordering guide will help you quickly choose and order the right columns, standards, reagents, and supplies for native SEC analysis of monoclonal antibodies
mAb Fragments and Dimers using SEC Workflow Ordering Guide (295.43 kB)
Workflow ordering guide will help you quickly choose and order the right columns, standards, reagents, and supplies for SEC analysis of mAb fragments and dimers
Agilent Bio HPLC Columns (26.00 MB)

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