puriFlash BIO Flash Columns for Peptide Purification from Advion-Interchim Scientific
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Discover high-performance biomolecule purification with puriFlash BIO flash columns by Advion-Interchim Scientific. These premium flash chromatography columns are specifically engineered for fast, efficient purification of peptides, proteins, oligonucleotides, and other biomolecules. Featuring reversed phase chemistries, puriFlash BIO columns deliver excellent resolution, high recovery, and consistent reproducibility – all essential for purification workflows. Available in multiple pore sizes and ligand chemistries including C18, C8, C4, they offer tailored solutions for peptide purification, protein purification, and oligonucleotide purification. Compatible with all major flash chromatography systems, puriFlash BIO flash columns streamline your workflow with faster separations, scalable purification, and robust performance – ideal for research laboratories, biotech, and pharmaceutical applications.
- puriFlash BIO 100 C18-N – Non-endcapped monofunctional C18 (100 Å); ideal for QA/QC and purification of polar and hydrophobic peptides <40 AA, up to 5 kDa, under pseudo-HILIC conditions (85–95% ACN)
- puriFlash BIO 100 C18-T – Endcapped tri-functional C18 (100 Å); optimised for purification of mid-polar to hydrophobic peptides <40 AA, up to 5 kDa
- puriFlash BIO 100 C18-XS – Multi-step endcapped monofunctional C18 (100 Å); for mid-polar to hydrophobic peptides <40 AA, up to 5 kDa, under basic conditions (up to pH 10)
- puriFlash BIO 200 C18-N – Non-endcapped monofunctional C18 (200 Å); suited for polar peptides <160 AA (up to 20 kDa) under pseudo-HILIC mode and hydrophobic peptides <80 AA (up to 10 kDa)
- puriFlash BIO 200 C18-T – Endcapped tri-functional C18 (200 Å); ideal for mid-polar to hydrophobic peptides <80 AA, up to 10 kDa
- puriFlash BIO 200 C18-XS – Multi-step endcapped monofunctional C18 (200 Å); for mid-polar to hydrophobic peptides <80 AA, up to 10 kDa, under basic conditions (up to pH 10)
- puriFlash BIO 200 C8-N – Non-endcapped monofunctional C8 (200 Å); for polar peptides <160 AA (up to 20 kDa) under pseudo-HILIC conditions and hydrophobic peptides <80 AA (up to 10 kDa)
- puriFlash BIO 300 C4-AQ – Hydrophilic C4 (300 Å); designed for large peptides and proteins >80 AA, up to 100 kDa, including fatty acids and natural peptides
Features
- Optimised for biomolecule purification – Engineered for peptides, proteins, oligonucleotides, and other biomolecules
- Wide range of chemistries – Available in C18, C8, and C4 ligands with tailored endcapping and bonding options
- Multiple pore sizes – Choose from 100 Å, 200 Å, and 300 Å to match target molecule size and application
- High-resolution separations – Precision-packed columns ensure sharp peaks and excellent resolution
- Robust hardware design – Durable column construction compatible with high flow rates and demanding flash systems
- Pseudo-HILIC capability – Efficient separation of polar peptides under high organic solvent conditions (85–95% ACN)
- Broad pH stability – Columns tolerate a wide pH range (up to pH 10 for XS variants) for diverse purification conditions
- Excellent reproducibility – Consistent batch-to-batch performance supports reliable QA/QC and purification workflows
- Scalable formats – Suitable for both analytical screening and preparative purification scales
- Seamless system compatibility – Integrates easily with Advion-Interchim and other major flash chromatography systems
Specification
| C18-N | C18-T | C18-XS | C8-N | C4-AQ | |
| Particle Type | Spherical | Spherical | Spherical | Spherical | Spherical |
| Particle Size | 15 µm, 30 µm | 15 µm, 30 µm | 15 µm, 30 µm | 15 µm, 30 µm | 15 µm, 30 µm |
| Pore Size | 100 Å, 200 Å | 100 Å, 200 Å | 100 Å, 200 Å | 200 Å | 300 Å |
| Endcapped | None | One-Step | Multi-Step | None | Mixed |
| pH Range | 1.5 - 8.0 | 1.5 - 8.0 | 1.0 - 10.0 | 1.5 - 8.0 | 1.5 - 8.0 |
