Uptisphere BIO Prep HPLC Columns from Advion-Interchim Scientific
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Uptisphere BIO preparative HPLC columns from Advion-Interchim Scientific are designed for the identification, separation, and purification of proteins, peptides, polypeptides, and biotherapeutics under both high- and low-pH conditions. The Uptisphere BIO family comprises three complementary ranges – X-Series, Uptisphere 300 Å, and Uptisphere TP – each offering distinct selectivity to address the complexities of biochromatography. With mono-, di-, and tri-functional bonding chemistries across C4, C8, and C18 alkyl chains, the Uptisphere BIO portfolio provides the versatility required for diverse biomolecular workflows. Columns are available in any length and in internal diameters from 4.6 mm to 50 mm, enabling smooth method development and scalable preparative purification.
Uptisphere Phase Options Tailored for Biomolecule Purification
- Uptisphere 300 Å WOD: A monofunctional 300 Å C18 phase, suited to weakly hydrophobic peptides and oligopeptides up to 50 kDa.
- Uptisphere 300 Å WC4: A monofunctional C4 phase with 300 Å pore size ideal for hydrophobic proteins and polypeptides in the 50–150 kDa range.
- Uptisphere 300 Å WD4: A polyfunctional C4 phase (300 Å), designed for the purification of hydrophobic proteins and polypeptides between 50 and 150 kDa.
- Uptisphere TP 18: A 300 Å C18 phase with polyfunctional bonding, suited to weakly hydrophobic peptides and oligopeptides up to 50 kDa.
- Uptisphere TP 14: A 300 Å C4 phase with polyfunctional bonding for hydrophobic proteins and polypeptides from 50 to 150 kDa.
- Uptisphere X-Series OD2: A 130 Å C18 phase featuring polyfunctional type II bonding with multi-step endcapping, offering an extended pH range of 1–13 for low molecular weight biotherapeutics.
- Uptisphere X-Series C18-AQ: A 220 Å C18 phase with polyfunctional type II bonding compatible with 100% aqueous mobile phases. Designed for mid-polar BioDrugs and medium molecular weight peptides.
- Uptisphere X-Series C8: A 220 Å C8 polyfunctional, multi-step endcapped phase intended for BioDrugs and peptides of medium molecular weight.
Features
- Comprehensive Biochromatography Selectivity – Eight phases spanning C4, C8, and C18 chemistries with mono-, di- and tri-functional bonding
- Designed for Proteins, Peptides & Polypeptides – Suitable for biomolecules ranging from small peptides to high-molecular-weight proteins
- Consistent Silica and Bonding from Analytical to Prep – Ensures smooth method transfer and reproducible purification performance
- Scalable Formats – Available in internal diameters from 4.6 – 50 mm for flexible method development and preparative workflows
Specification
| WOD | WC4 | WD4 | TP 18 | TP 14 | X-Serie OD2 | X-Serie C18-AQ | X-Serie C8 | |
| Phase | C18 | C4 | C4 | C18 | C4 | C18 | C18 | C8 |
| Bonding | Mono-functional | Mono-functional | Poly-functional | Poly-functional | Poly-functional | Poly-functional Type II | Poly-functional Type II | Poly-functional Type II |
| Mode | Reversed Phase | Reversed Phase | Reversed Phase | Reversed Phase | Reversed Phase | Reversed Phase | Reversed Phase | Reversed Phase |
| Particle Type | Fully Porous | Fully Porous | Fully Porous | Fully Porous | Fully Porous | Fully Porous | Fully Porous | Fully Porous |
| Particle Size | 5 µm | 5 µm | 5 µm | 5 µm, 10 µm, 15 µm | 5 µm, 10 µm, 15 µm | 5 µm | 5 µm | 5 µm |
| Pore Size | 300 Å | 300 Å | 300 Å | 300 Å | 300 Å | 130 Å | 220 Å | 220 Å |
| Surface Area | 100 m2/g | 100 m2/g | 100 m2/g | 100 m2/g | 100 m2/g | 300 m2/g | 200 m2/g | 200 m2/g |
| Carbon Load | 10% | 4% | 4% | 8% | 3.5% | 20% | 14% | 8% |
| Endcapped | One-step | One-step | One-Step | One-step | One-step | Multi-step | Mixed | Multi-step |
| pH Range | 1.5 - 7 | 2 - 7 | 1.5 - 8 | 1.5 - 7.5 | 2 - 7 | 1 - 13 | 1 - 10 | 1 - 13 |
| USP Designation | L1 | L26 | L26 | L1 | L26 | L1 | L1 | L7 |
