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Agilent Technologies Poroshell 120

Poroshell 120 columns provide exceptional efficiency for standard HPLC systems and significantly boost the performance from UHPLC instruments.

A key feature of Poroshell columns is their superficially porous microparticulate column packing.

Poroshell 120 particles have a 1.7 μm solid silica core with a 0.5 μm porous outer layer. This unique configuration gives you all the performance advantages of sub-2 μm particles with backpressures comparable to a sub-3 μm particle.

New - now available with 4 µm particle size!

Advantages of Poroshell 120

  • Faster and more efficient separations than Standard HPLC columns, without the need for expensive UPLC instrumentation
  • Efficiencies comparable to 1.8 μm coluns
  • Can be used on all HPLC systems- no need to invest in UPLC equipment
  • Increase in separation speed and efficiency can result in better sensitivity and lower limits of detection
  • Agilent's Zorbax Sol Gel Technology is used to coat the solid core in a single process providing excellent mechanical robustness
  • Can be used on standard HPLC instrumentation with 400 and 600 bar ratings.

The core shell technology of Poroshell improves separation efficiency and decreases analysis times on standard HPLC equipment. The pore size is smaller in comparison to totally porous silica leading to reduced mass transfer effects meaning that separation happens faster.

 



By its nature this type of particle has a very narrow particle size distribution. The diffusion path lengths of all molecules through the column become more uniform - leading to very efficient peaks.


Poroshell 120 - narrow particle size distribution

As the particle size is 2.7 μm and because less mobile phase is required to fill the column- the back pressure is only 40-50% compared to <2 μm particle columns.
Bonded Phase Pore Size (Å) Temp. Limits (°C) pH Range Endcapped Carbon Load (%)
EC-C18 120 60 2.0-8.0 Yes 8.0
EC-C8 120 60 2.0-8.0 Yes 5.0
Phenyl-Hexyl 120 60 2.0-8.0 Yes 8.0
SB-C18 120 90 1.0-8.0 No 7.5
SB-C8 120 80 1.0-8.0 No 4.5
HPH-C18 100 60 3.0-11.0 Double Proprietry
HPH-C8 100 60 3.0-11.0 Double Proprietry
SB-Aq 120 80 1.0-8.0 No Proprietry
Bonus-RP 120 60 2.0-9.0 Triple 5.0
EC-CN 120 60 2.0-8.0 Yes 3.5
PFP 120 60 2.0-8.0 Yes 5.1
AdvanceBio 120 60 2.0-8.0 Yes 8.0

Poroshell 120 EC-C18 (USP L1)

Particle size (µm) Length (mm) ID (mm)
2.1 3.0 4.6
         
2.7 30
691775-902
691975-302
691975-902
50
699775-902
699975-302
699975-902
75
697775-902
697975-302
697975-902
100
695775-902
695975-302
695975-902
150
693775-902
693975-302
693975-902
5 (guard)
821725-911
823750-911
820750-911
         
4.0 50
699770-902
699970-302
699970-902
100
695770-902
695970-302
695970-902
150
693770-902
693970-302
693970-902
250
650750-902
690970-302
690970-902
5 (guard)
821725-916
823750-916
820750-916

 

Poroshell 120 EC-C8 (USP L7)

Particle size (µm) Length (mm) ID (mm)
2.1 3.0 4.6
         
2.7 30
691775-906
691975-306
691975-906
50
699775-906
699975-306
699975-906
75
697775-906
697975-306
697975-906
100
695775-906
695975-306
695975-906
150
693775-906
693975-306
693975-906
5 (guard)
821725-913
823750-913
820750-913
         
4.0 50
699770-906
699970-306
699970-906
100
695770-906
695970-306
695970-906
150
693770-906
693970-306
693970-906
250
650750-906
690970-306
690970-906

 

Poroshell 120 Phenyl Hexyl (USP L11)

Particle size (µm) Length (mm) ID (mm)
2.1 3.0 4.6
         
2.7 50
699775-912
699975-312
699975-912
100
695775-912
695975-312
695975-912
150
693775-912
693975-312
693975-912
5 (guard)
821725-914
823750-914
820750-914
         
4.0 50
699770-912
699970-312
699970-912
100
695770-912
695970-312
695970-912
150
693770-912
693970-312
693970-912
250
650750-912
690970-312
690970-912

 

Poroshell 120 SB-C18 (USP L1)

Particle size (µm) Length (mm) ID (mm)
2.1 3.0 4.6
         
2.7 30
681775-902
681975-302
681975-902
50
689775-902
689975-302
689975-902
75
687775-902
687975-302
687975-902
100
685775-902
685975-302
685975-902
150
683775-902
683975-302
683975-902
5 (guard)
821725-912
823750-912
820750-912

 

Poroshell 120 SB-C8 (USP L7)

Particle size (µm) Length (mm) ID (mm)
2.1 3.0 4.6
         
2.7 50
689775-906
689975-306
689975-906
100
685775-906
685975-306
685975-906
150
683775-906
683975-306
683975-906

 

Poroshell 120 HPH-C18 (USP L1)

Particle size (µm) Length (mm) ID (mm)
2.1 3.0 4.6
         
2.7 50
699775-702
699975-502
699975-702
100
695775-702
695975-502
695975-702
150
693775-702
693975-502
693975-702

 

Poroshell 120 HPH-C8 (USP L7)

Particle size (µm) Length (mm) ID (mm)
2.1 3.0 4.6
         
2.7 50
699775-706
699975-506
699975-706
100
695775-706
695975-506
695975-706
150
693775-706
693975-506
693975-706

 

Poroshell 120 SB-Aq

Particle size (µm) Length (mm) ID (mm)
2.1 3.0 4.6
         
2.7 50
689775-914
689975-314
689975-914
100
685775-914
685975-314
685975-914
150
683775-914
683975-314
683975-914

 

Poroshell 120 Bonus-RP

Particle size (µm) Length (mm) ID (mm)
2.1 3.0 4.6
         
2.7 50
699768-901
699968-301
699968-901
100
695768-901
695968-301
695968-901
150
693768-901
693968-301
693968-901

 

Poroshell HILIC

Particle size (µm) Length (mm) ID (mm)
2.1 3.0 4.6
         
2.7 50
699775-901
699975-301
699975-901
100
695775-901
695975-301
695975-901
150
693775-901
693975-301
693975-901
         
4.0 50
699770-901
699970-301
699970-901
100
695770-901
695970-301
695970-901
150
693770-901
693970-301
696970-901
250
650750-901
690970-301
690970-901

 

Poroshell EC-CN

Particle size (µm) Length (mm) ID (mm)
2.1 3.0 4.6
         
2.7 50
699775-905
699975-305
699975-905
100
695775-905
695975-305
695975-905
150
693775-905
693975-305
693975-905

 

Poroshell 120 PFP

Particle size (µm) Length (mm) ID (mm)
2.1 3.0 4.6
         
2.7 50
699775-408
699975-308
699975-408
100
695775-408
695975-308
695975-408
150
693775-408
693975-308
693975-408
5 (guard)
821725-915
823750-915
820750-915
         
4.0 50
699770-408
699970-308
699970-408
100
695770-408
695970-308
695970-408
150
693770-408
693970-308
693970-408
250
650750-408
690970-308
690970-408

 

Length (mm) Internal Diameter (mm)
2.1 3.0 4.6
100
655750-902
   
150
653750-902
653950-302
653950-902
250
651750-902
   
Fast Guards
851725-911
853750-911
850750-911

CLICK HERE for more information on Agilent's New AdvanceBio Peptide Mapping Columns

The Poroshell Difference
Poroshell Van Deemter
For Poroshell applications CLICK HERE to search the Crawford Scientific online applications database
 

 
 
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