Gilson FC 204 Fraction Collector
The Gilson FC 204 Fraction Collector is a high-capacity system designed for fraction collection across a wide range of liquid chromatography applications, from analytical to preparative flow rates. It is typically used in conjunction with a peristaltic pump and supports both standalone operation and control via TRILUTION LC software. The built-in keypad provides direct, local control for method setup and routine operation, while multiple input/output connections allow straightforward integration with FPLC, flash chromatography and HPLC systems.
With a compact footprint suitable for use in fume hoods and biosafety cabinets, the FC 204 is designed for safe handling of hazardous or biological materials. The system is supplied with a 3-way diverter valve, allowing column void volumes, equilibration phases and non-relevant peaks to be diverted to waste, improving collection efficiency. Two model variants are available to support different flow rate requirements, ensuring appropriate performance for both lower-flow analytical applications and higher-flow preparative separations.
All pictures shown are for illustration purpose only. Actual product may vary.
Features
- Model Variants Available – Supplied as FC 204 (part number 171041-GIL) for flow rates up to 20 mL/min, or FC 204 (part number 171043-GIL) for flow rates up to 200 mL/min
- Wide Flow Rate Compatibility – Suitable for analytical to preparative flow rates when paired with a peristaltic pump
- 3-Way Diverter Valve – Enables diversion of void volumes, equilibration phases or unwanted peaks to waste, and
- Standalone or Software-Controlled Operation – Operates independently via the integrated keypad or under TRILUTION LC software control
- High-Capacity Collection – Holds four racks accommodating up to 240 fractions (13 × 100 mm tubes), up to 176 fractions (18 × 180 mm tubes), or up to eight shallow- or deep-well microplates
- Multicolumn Collection Capability – Optional multicolumn adapter supports parallel fraction collection from up to 18 independent channels
- System Integration – Multiple input/output contacts allow easy connection to a wide range of chromatography systems, including FPLC, flash chromatography and HPLC
- Compact, Contamination-Conscious Design – Small footprint suitable for fume hoods and biosafety cabinets, with an easy-to-sterilise flow path to reduce contamination risk
Specification
| FC 204 Fraction Collector | |
| 3-way Valves | 3-port, PTFE, 114 μL internal volume, 6 μL dead volume from common port to the normally closed port, up to 200 mL/min with large-bore needle |
| Communication Interface | GSIOC; Inputs for start/advance and end/home; output for control of peripheral devices |
| Data Acquisition | An input channel for analog-to-digital conversion; digital data is transmitted on the GSIOC |
| Detector Input | ±100 mV full scale |
| Drop Counting | Up to 9,999 drops per fraction Maximum rate: 20 drops/sec |
| Event Marker | 100 ms pulse |
| Front Panel | Five commands, HELP, YES, NO, and 0–9 numeric hard keys; four soft keys |
| Display Panel | Two-line, 24-character display on backlit LCD |
| Manufacturing Standards | Meets applicable Safety and EMC certification standards; UL and CE certified |
| Maximum Collection Volume/Tube | 32 mL tubes (18 x 180 mm) or 25 mL tubes (18 x 50 mm) |
| Maximum Fractions | Up to 768 when configured with eight 96-well, 2-mL Whatman/Polyfiltronics microplates on a Code 17 rack |
| Multiple-Column Collection | Simultaneous collection from up to 18 columns with installation of optional multiple column adaptor(s) |
| Multiple-Cycle Operation | Repetitive collection of each sample into same set of tubes or collection of each sample into different set of tubes |
| Number of Racks | Holds one Code 16 or Code 16D rack, one Code 17 rack or up to four Code 20-Series racks (all racks must be the same code) |
| Operating Modes | Drop, Manual, Peak + Drop, Peak + Time, Time |
| Peak Detection | Adaptive-slope algorithm that applies user-specified parameters to accommodate drifting baselines, negative, and asymmetrical peaks, or absolute threshold level that collects all peaks above specified mV value Peak parameters: peak height or level, in mV; peak width at half-height, in minutes Minimum peak height for fractionation: 0.1 mV full scale Detector-collector delay: 0.01 minute increments |
| Power Requirements | Frequency: 50–60 Hz Voltage: 100–120V or 220–240V; main voltage fluctuations not to exceed ±10% of the nominal voltage Current rating: 0.5A for 100–120V, or 0.25A for 220–240V |
| Programmable Time Units | From 0.01 to 99.99 minutes per tube with a 0.01 minute limit of resolution |
| Software | Via TRILUTION LC Software |
| Time-Based Programming | Up to 10 collection windows and 10 drain steps in any mode |
| Tube Change Time | 100–720 ms center to center, depending on rack type |
| Zero Power Memory | Maintains memory for a minimum of five years from date of shipment |
| Weight | 10.9 kg |
| Dimensions | Width: 47.9 cm Depth: 46.4 cm Height: 33.0 cm |
