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Agilent Bio SEC Columns

Agilent Bio SEC HPLC Columns

Agilent Bio SEC offer fast, reliable, accurate performance for your biopharmaceutical analysis. They are easy to integrate into your workflow, and are available in a full range of pore sizes and dimensions to ensure the perfect separation every time.

Agilent Bio SEC-3

These columns are a breakthrough technology for size exclusion chromatography (SEC). They are packed with spherical, narrowly dispersed 3 µm silica particles coated with a proprietary hydrophilic layer. This thin polymeric layer is chemically bonded to pure, mechanically stable silica under controlled conditions, ensuring a highly efficient size exclusion particle.

Available in 100Å, 150Å and 300Å pore sizes to accommodate most peptide and protein size exclusion separations.

Agilent Bio SEC-5

These columns are packed with 5 µm silica particles coated with a proprietary, neutral, hydrophilic layer for maximum efficiency and stability. Our specially designed packing also promotes high pore volume, improving both peak capacity and resolution.

Available in 100Å, 150Å, 300Å, 500Å, 1000Å and 2000Å.

Agilent Bio SEC columns offer fast, reliable, accurate performance for your biopharmaceutical analysis. They are easy to integrate into your workflow and are available in a full range of pore sizes and dimensions to ensure the perfect separation every time.

Bio SEC-3 HPLC bio columns are a breakthrough technology for size exclusion chromatography. These bio columns are packed with spherical, narrowly dispersed 3 μm silica particles coated with a proprietary hydrophilic layer. This thin polymeric layer is chemically bonded to pure, mechanically stable silica under controlled conditions, ensuring an efficient size exclusion particle. Bio SEC-3 HPLC columns are available in 100Å, 150Å and 300Å pore sizes to accommodate most peptide and protein size exclusion separations.

Bio SEC-5 HPLC size exclusion chromatography columns are packed with 5 μm silica particles coated with a proprietary, neutral, hydrophilic layer for maximum efficiency and stability. Specially designed packing also promotes high pore volume, improving both peak capacity and resolution. Bio SEC-5 HPLC columns are available in 100 Å, 150 Å, 300 Å, 500 Å, 1000 Å, and 2000 Å.

Part Number Short Description Particle Size ID Length Pore Size Type Price Qty
5190-6850 Agilent Bio SEC-3 100A 21.2 x 300mm Column 3 um 21.2 mm 300 mm 100 A Column
£13,344.00
5190-6854 Agilent Bio SEC-3 100A 21.2 x 50mm Column 3 um 21.2 mm 50 mm 100 A Column
£3,195.00
5190-2504 Agilent Bio SEC-3 100A 4.6 x 150mm Column 3 um 4.6 mm 150 mm 100 A Column
£1,854.00
5190-2503 Agilent Bio SEC-3 100A 4.6 x 300mm Column 3 um 4.6 mm 300 mm 100 A Column
£2,094.00
5190-6846 Agilent Bio SEC-3 100A 4.6 x 50mm Guard 3 um 4.6 mm 50 mm 100 A Guard
£784.00
5190-2502 Agilent Bio SEC-3 100A 7.8 x 150mm Column 3 um 7.8 mm 150 mm 100 A Column
£1,819.00
5190-2501 Agilent Bio SEC-3 100A 7.8 x 300mm Column 3 um 7.8 mm 300 mm 100 A Column
£2,396.00
5190-2505 Agilent Bio SEC-3 100A 7.8 x 50mm Guard 3 um 7.8 mm 50 mm 100 A Guard
£784.00
5190-6851 Agilent Bio SEC-3 150A 21.2 x 300mm Column 3 um 21.2 mm 300 mm 150 A Column
£13,344.00
5190-6855 Agilent Bio SEC-3 150A 21.2 x 50mm Column 3 um 21.2 mm 50 mm 150 A Column
£3,195.00
5190-2509 Agilent Bio SEC-3 150A 4.6 x 150mm Column 3 um 4.6 mm 150 mm 150 A Column
£1,854.00
5190-2508 Agilent Bio SEC-3 150A 4.6 x 300mm Column 3 um 4.6 mm 300 mm 150 A Column
£2,094.00
5190-6847 Agilent Bio SEC-3 150A 4.6 x 50mm Guard 3 um 4.6 mm 50 mm 150 A Guard
£784.00
5190-2507 Agilent Bio SEC-3 150A 7.8 x 150mm Column 3 um 7.8 mm 150 mm 150 A Column
£1,819.00
5190-2506 Agilent Bio SEC-3 150A 7.8 x 300mm Column 3 um 7.8 mm 300 mm 150 A Column
£2,396.00
5190-2510 Agilent Bio SEC-3 150A 7.8 x 50mm Guard 3 um 7.8 mm 50 mm 150 A Guard
£784.00
5190-6852 Agilent Bio SEC-3 300A 21.2 x 300mm Column 3 um 21.2 mm 300 mm 300 A Column
£13,344.00
5190-6856 Agilent Bio SEC-3 300A 21.2 x 50mm Column 3 um 21.2 mm 50 mm 300 A Column
£3,195.00
5190-2514 Agilent Bio SEC-3 300A 4.6 x 150mm Column 3 um 4.6 mm 150 mm 300 A Column
£1,854.00
5190-2513 Agilent Bio SEC-3 300A 4.6 x 300mm Column 3 um 4.6 mm 300 mm 300 A Column
£2,094.00
5190-6848 Agilent Bio SEC-3 300A 4.6 x 50mm Guard 3 um 4.6 mm 50 mm 300 A Guard
£784.00
5190-2512 Agilent Bio SEC-3 300A 7.8 x 150mm Column 3 um 7.8 mm 150 mm 300 A Column
£1,819.00
5190-2511 Agilent Bio SEC-3 300A 7.8 x 300mm Column 3 um 7.8 mm 300 mm 300 A Column
£2,396.00
5190-2511K Agilent Bio SEC-3 300A 7.8 x 300mm Method Validation Kit 3 um 7.8 mm 300 mm 300 A Validation Kit
£7,187.00
5190-2515 Agilent Bio SEC-3 300A 7.8 x 50mm Guard 3 um 7.8 mm 50 mm 300 A Guard
£784.00
5190-6867 Agilent Bio SEC-5 1000A 21.2 x 300mm Column 5 um 21.2 mm 300 mm 1000 A Column
£9,477.00
5190-6873 Agilent Bio SEC-5 1000A 21.2 x 50mm Column 5 um 21.2 mm 50 mm 1000 A Column
£2,328.00
5190-2539 Agilent Bio SEC-5 1000A 4.6 x 150mm Column 5 um 4.6 mm 150 mm 1000 A Column
£1,093.00
5190-2538 Agilent Bio SEC-5 1000A 4.6 x 300mm Column 5 um 4.6 mm 300 mm 1000 A Column
£1,404.00
5190-6861 Agilent Bio SEC-5 1000A 4.6 x 50mm Guard 5 um 4.6 mm 50 mm 1000 A Guard
£549.00
5190-2537 Agilent Bio SEC-5 1000A 7.8 x 150mm Column 5 um 7.8 mm 150 mm 1000 A Column
£1,310.00
5190-2536 Agilent Bio SEC-5 1000A 7.8 x 300mm Column 5 um 7.8 mm 300 mm 1000 A Column
£1,715.00
5190-2540 Agilent Bio SEC-5 1000A 7.8 x 50mm Guard 5 um 7.8 mm 50 mm 1000 A Guard
£566.00
5190-6863 Agilent Bio SEC-5 100A 21.2 x 300mm Column 5 um 21.2 mm 300 mm 100 A Column
£9,247.00
5190-6869 Agilent Bio SEC-5 100A 21.2 x 50mm Column 5 um 21.2 mm 50 mm 100 A Column
£2,340.00
5190-2519 Agilent Bio SEC-5 100A 4.6 x 150mm Column 5 um 4.6 mm 150 mm 100 A Column
£1,153.00
5190-2518 Agilent Bio SEC-5 100A 4.6 x 300mm Column 5 um 4.6 mm 300 mm 100 A Column
£1,424.00
5190-6857 Agilent Bio SEC-5 100A 4.6 x 50mm Guard 5 um 4.6 mm 50 mm 100 A Guard
£551.00
5190-2517 Agilent Bio SEC-5 100A 7.8 x 150mm Column 5 um 7.8 mm 150 mm 100 A Column
£1,238.00
5190-2516 Agilent Bio SEC-5 100A 7.8 x 300mm Column 5 um 7.8 mm 300 mm 100 A Column
£1,723.00
5190-2520 Agilent Bio SEC-5 100A 7.8 x 50mm Guard 5 um 7.8 mm 50 mm 100 A Guard
£562.00
5190-6864 Agilent Bio SEC-5 150A 21.2 x 300mm Column 5 um 21.2 mm 300 mm 150 A Column
£9,247.00
5190-6870 Agilent Bio SEC-5 150A 21.2 x 50mm Column 5 um 21.2 mm 50 mm 150 A Column
£2,340.00
5190-2524 Agilent Bio SEC-5 150A 4.6 x 150mm Column 5 um 4.6 mm 150 mm 150 A Column
£1,153.00
5190-2523 Agilent Bio SEC-5 150A 4.6 x 300mm Column 5 um 4.6 mm 300 mm 150 A Column
£1,424.00
5190-6858 Agilent Bio SEC-5 150A 4.6 x 50mm Guard 5 um 4.6 mm 50 mm 150 A Guard
£551.00
5190-2522 Agilent Bio SEC-5 150A 7.8 x 150mm Column 5 um 7.8 mm 150 mm 150 A Column
£1,238.00
5190-2521 Agilent Bio SEC-5 150A 7.8 x 300mm Column 5 um 7.8 mm 300 mm 150 A Column
£1,723.00
5190-2525 Agilent Bio SEC-5 150A 7.8 x 50mm Guard 5 um 7.8 mm 50 mm 150 A Guard
£562.00
5190-6868 Agilent Bio SEC-5 2000A 21.2 x 300mm Column 5 um 21.2 mm 300 mm 2000 A Column
£10,811.00
5190-6874 Agilent Bio SEC-5 2000A 21.2 x 50mm Column 5 um 21.2 mm 50 mm 2000 A Column
£2,546.00
5190-2544 Agilent Bio SEC-5 2000A 4.6 x 150mm Column 5 um 4.6 mm 150 mm 2000 A Column
£1,093.00
5190-2543 Agilent Bio SEC-5 2000A 4.6 x 300mm Column 5 um 4.6 mm 300 mm 2000 A Column
£1,404.00
5190-6862 Agilent Bio SEC-5 2000A 4.6 x 50mm Guard 5 um 4.6 mm 50 mm 2000 A Guard
£549.00
5190-2542 Agilent Bio SEC-5 2000A 7.8 x 150mm Column 5 um 7.8 mm 150 mm 2000 A Column
£1,310.00
5190-2541 Agilent Bio SEC-5 2000A 7.8 x 300mm Column 5 um 7.8 mm 300 mm 2000 A Column
£1,715.00
5190-2545 Agilent Bio SEC-5 2000A 7.8 x 50mm Guard 5 um 7.8 mm 50 mm 2000 A Guard
£566.00
5190-6865 Agilent Bio SEC-5 300A 21.2 x 300mm Column 5 um 21.2 mm 300 mm 300 A Column
£9,247.00
5190-6871 Agilent Bio SEC-5 300A 21.2 x 50mm Column 5 um 21.2 mm 50 mm 300 A Column
£2,340.00
5190-2529 Agilent Bio SEC-5 300A 4.6 x 150mm Column 5 um 4.6 mm 150 mm 300 A Column
£1,153.00
5190-2528 Agilent Bio SEC-5 300A 4.6 x 300mm Column 5 um 4.6 mm 300 mm 300 A Column
£1,424.00
5190-6859 Agilent Bio SEC-5 300A 4.6 x 50mm Guard 5 um 4.6 mm 50 mm 300 A Guard
£551.00
5190-2527 Agilent Bio SEC-5 300A 7.8 x 150mm Column 5 um 7.8 mm 150 mm 300 A Column
£1,238.00
5190-2526 Agilent Bio SEC-5 300A 7.8 x 300mm Column 5 um 7.8 mm 300 mm 300 A Column
£1,723.00
5190-2530 Agilent Bio SEC-5 300A 7.8 x 50mm Guard 5 um 7.8 mm 50 mm 300 A Guard
£562.00
5190-6866 Agilent Bio SEC-5 500A 21.2 x 300mm Column 5 um 21.2 mm 300 mm 500 A Column
£9,477.00
5190-6872 Agilent Bio SEC-5 500A 21.2 x 50mm Column 5 um 21.2 mm 50 mm 500 A Column
£2,328.00
5190-2534 Agilent Bio SEC-5 500A 4.6 x 150mm Column 5 um 4.6 mm 150 mm 500 A Column
£1,093.00
5190-2533 Agilent Bio SEC-5 500A 4.6 x 300mm Column 5 um 4.6 mm 300 mm 500 A Column
£1,404.00
5190-6860 Agilent Bio SEC-5 500A 4.6 x 50mm Guard 5 um 4.6 mm 50 mm 500 A Guard
£549.00
5190-2532 Agilent Bio SEC-5 500A 7.8 x 150mm Column 5 um 7.8 mm 150 mm 500 A Column
£1,310.00
5190-2531 Agilent Bio SEC-5 500A 7.8 x 300mm Column 5 um 7.8 mm 300 mm 500 A Column
£1,715.00
5190-2535 Agilent Bio SEC-5 500A 7.8 x 50mm Guard 5 um 7.8 mm 50 mm 500 A Guard
£566.00
  • Exceptional loading capacity, stability, and reproducibility for size-based biomolecule separations
  • Sharper peaks, higher resolution, and better protein recovery
  • Faster separations than large-particle SEC columns
  • Compatibility with most aqueous buffers
  • Excellent stability in high-salt and low-salt conditions
  • Wide range of pore size options, including 1000 Å and 2000 Å gives options for everything from peptides to VLPs
  • Analytical and semi-prep dimensions for easy scale up or down
  Bio SEC-3 Bio SEC-5
Pore Size 100 Å 150 Å 300 Å 100 Å 150 Å 300 Å 500 Å 1000 Å 2000 Å
Particle Size 3 µm 3 µm 3 µm 5 µm 5 µm 5 µm 5 µm 5 µm 5 µm
Particle Type Fully Porous Fully Porous Fully Porous Fully Porous Fully Porous Fully Porous Fully Porous Fully Porous Fully Porous
Molecular Weight Lower Limit 100 Da 500 Da 5000 Da 100 Da 500 Da 5000 Da 15,000 Da 50,000 Da  
Molecular Weight Upper Limit 100,000 Da 150,000 Da 1,250,000 Da 100,000 Da 150,000 Da 1,250,000 Da 5,000,000 Da 7,500,000 Da >10,000,000 Da
Temperature Limit 80°C 80°C 80°C 80°C 80°C 80°C 80°C 80°C 80°C
pH range 2 - 8.5     2 - 8.5     2 - 8.5     2 - 8.5     2 - 8.5     2 - 8.5     2 - 8.5     2 - 8.5     2 - 8.5    
Aggregate Analysis Application Compendium (6.82 MB)
Collection of Agilent app notes on aggregate/fragment analysis
Agilent Bio HPLC Columns (26.00 MB)

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