Agilent HPLC Column Method Development Kits

Method development is some of the most important work you do. Now, it's easier than ever to evaluate the best chemistries for your separation with Agilent Method Development Kits. These kits include a range of column chemistries and selectivities, giving you the tools you need to save time and perfect your separation.

Agilent's columns are manufactured to the industry's tightest quality control specifications for reliable, reproducible performance. We also offer the industry's widest range of Fast LC columns to help you get high resolution and speedy analysis times.

Agilent method development kits help to simplify method development. The InfinityLab Poroshell and ZORBAX columns compiled in these kits were carefully selected to reflect tasks in your everyday method development, such as screening solvents or orthogonal stationary phases for analytical LC method development. Agilent columns are manufactured to the industry’s tightest quality control specifications for reliable, reproducible performance, making them ideal for LC method development.

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Buy

Part Number	Short Description	Phase	Particle Size	ID	Length	Price
5190-6157	Poroshell 120 Aqueous 2.1 x 50mm Method Development Kit	Aqueous	2.7 um	2.1 mm	50 mm	£1,141.00
5190-6158	Poroshell 120 Aqueous 4.6 x 50mm Method Development Kit	Aqueous	2.7 um	4.6 mm	50 mm	£1,152.00
5190-6155	Poroshell 120 Selectivity 2.1 x 50mm Method Development Kit	Selectivity	2.7 um	2.1 mm	50 mm	£1,152.00
5190-6156	Poroshell 120 Selectivity 4.6 x 50mm Method Development Kit	Selectivity	2.7 um	4.6 mm	50 mm	£1,152.00
5190-6160	Poroshell 120 USP 3 x 100mm Method Development Kit	USP	2.7 um	3.0 mm	100 mm	£1,410.00
5190-6159	Poroshell 120 USP 4.6 x 100mm Method Development Kit	USP	2.7 um	4.6 mm	100 mm	£1,410.00
5185-5809	ZORBAX Aqueous 5um 4.6 x 150mm Method Development Kit	Aqueous	5 um	4.6 mm	150 mm	£991.00
5183-4627	ZORBAX Eclipse XDB Fast 5um 4.6 x 75mm Method Development Kit	Fast	5 um	4.6 mm	75 mm	£972.00
5183-4626	ZORBAX Eclipse XDB Traditional 5um 4.6 x 150mm Method Development Kit	Traditional	5 um	4.6 mm	150 mm	£991.00
5185-5810	ZORBAX Fast Aqueous 3.5um 4.6 x 75mm Method Development Kit	Aqueous	3.5 um	4.6 mm	75 mm	£972.00
5185-5808	ZORBAX Fast pH 3.5um 4.6 x 75mm Method Development Kit	pH	3.5 um	4.6 mm	75 mm	£972.00
5190-1436	ZORBAX pH 3.5um 4.6 x 100mm Method Development Kit	pH	3.5 um	4.6 mm	100 mm	£1,296.00
5185-5807	ZORBAX pH 5um 4.6 x 150mm Method Development Kit	pH	5 um	4.6 mm	150 mm	£972.00
5190-6154	ZORBAX RRHD Aqueous 1.8um 2.1 x 50mm Method Development Kit	Aqueous	1.8 um	2.1 mm	50 mm	£1,388.00
5190-6153	ZORBAX RRHD Eclipse Plus 1.8um 2.1 x 50mm Method Development Kit	Fast	1.8 um	2.1 mm	50 mm	£1,401.00
5190-6152	ZORBAX RRHD pH 1.8um 2.1 x 50mm Method Development Kit	pH	1.8 um	2.1 mm	50 mm	£1,388.00
5190-1432	ZORBAX RRHT pH 1.8um 2.1 x 50mm Method Development Kit	pH	1.8 um	2.1 mm	50 mm	£1,271.00
5190-1434	ZORBAX RRHT pH 1.8um 4.6 x 50mm Method Development Kit	pH	1.8 um	4.6 mm	50 mm	£1,271.00
5190-1431	ZORBAX RRHT Selectivity 1.8um 2.1 x 50mm Method Development Kit	Selectivity	1.8 um	2.1 mm	50 mm	£1,258.00
5190-1433	ZORBAX RRHT Selectivity 1.8um 4.6 x 50mm Method Development Kit	Selectivity	1.8 um	4.6 mm	50 mm	£1,271.00
5190-1435	ZORBAX Selectivity 3.5um 4.6 x 100mm Method Development Kit	Selectivity	3.5 um	4.6 mm	100 mm	£1,296.00
5183-4625	ZORBAX StableBond Fast 5um 4.6 x 75mm Method Development Kit	Fast	5 um	4.6 mm	75 mm	£962.00
5183-4624	ZORBAX StableBond Traditional 5um 4.6 x 150mm Method Development Kit	Traditional	5 um	4.6 mm	150 mm	£991.00

Features

Method Development Kits allow for fast method development at an attractive price
Each kit contains 3 columns in a variety of bonded phases for easy method optimisation
Choice of Poroshell or Zorbax phases
2.1 x 50 mm, 1.8 µm – is ideal for high-speed, high-resolution separations on optimized, high pressure LC Systems and especially LC-MS Systems
4.6 x 50 mm, 1.8 µm – is best for high-speed, high-resolution separations with operating pressures below 600 bar
4.6 x 100 mm, 3.5 µm – offers superior instrument compatibility and easy method transfer, while allowing you to scale up to 5 µm or down to 1.8 µm without revalidation

Specifications

Poroshell 120

Superficially porous particles for high efficiency at low pressure
Sub-2 µm efficiency with a 2.7 µm particle
Compatible with 400 bar and 600 bar LC's

ZORBAX Eclipse Plus

High resolution and efficiency with 1.8, 3.5 and 5 µm columns
1.8 um columns available in RRHD (1200 bar) and RRHT (600 bar) formats
Excellent first choice for method development
Long life from pH 2-9 for reliable separations of basic, acidic and neutral compounds
Rigorous QA/QC testing for greater long-term reproducibility

ZORBAX Eclipse XDB

High performance over a wide pH range (2-9)
Good peak shape for acids, bases and neutrals
Long lifetime with eXtra Dense Bonding and double endcapping

ZORBAX StableBond

Exceptional stability at low pH (1-2)
Patented stable column chemistry allows use at a high temperature and low pH without degradation

ZORBAX Bonus-RP

Excellent peak shape for challenging basic compounds at low and mid pH
Unique reversed-phase selectivity
Novel bonding technology with embedded polar group and steric protection
Usable in 100% aqueous mobile phase

ZORBAX Extend-C18

Separating basic compounds above their pka in free base form; separation of basic, acidic, neutral compounds at high pH (up to pH 11.5)
Uses ammonium hydroxide as mobile phase additive with LC-MS with small molecules or peptides

Literature

Poroshell 120 Poster (3.44 MB)

More chemistries for solving your toughest separation challenges

LC Method Development Applications Notebook (8.40 MB)

Creating consistent, efficient, and cost-effective analytical LC methods

Zorbax Columns Poster (2.76 MB)

A proven and reliable portfolio of totally porous HPLC columns