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Agilent PL-SAX Columns

Agilent PL-SAX columns are ideal for the anion-exchange HPLC separations of proteins, peptides, and deprotected synthetic oligonucleotides under denaturing conditions. The strong anion-exchange functionality, covalently linked to a chemically stable fully porous polymer, extends the operating pH range. In addition, the anion-exchange capacity is independent of pH. 

For synthetic oligonucleotides, separations using denaturing conditions of temperature, organic solvent, and high pH are all possible. PL-SAX delivers improved chromatography for self-complementary or G-rich sequences that may associate to form aggregates or hairpin structures. The 5 μm material provides high-efficiency separations of n and n-1 sequences.

A wide range of particle sizes and column geometries permits analysis scale-up to purification. The strong anion-exchange functionality provides a material with exceptional chemical and thermal stability, even with sodium hydroxide eluents, leading to long column lifetime.

 

Part Number Short Description Phase Particle Size ID Length Pore Size Type Price Qty
PL1251-3102 PL-SAX 1000A 10um 150 x 25mm Column PL-SAX 10 um 25.0 mm 150 mm 1000 A Column
£3,249.00
PL1551-3102 PL-SAX 1000A 10um 150 x 4.6mm Column PL-SAX 10 um 4.6 mm 150 mm 1000 A Column
£684.00
PL1751-3102 PL-SAX 1000A 10um 150 x 50mm Column PL-SAX 10 um 50.0 mm 150 mm 1000 A Column
£10,863.00
PL1551-5102 PL-SAX 1000A 10um 250 x 4.6mm Column PL-SAX 10 um 4.6 mm 250 mm 1000 A Column
£812.00
PL1851-2102 PL-SAX 1000A 10um 300 x 100mm Column PL-SAX 10 um 100.0 mm 300 mm 1000 A Column
£30,478.00
PL1651-0102 PL-SAX 1000A 10um 5 x 3mm Guard Cartridge 2pk PL-SAX 10 um 3.0 mm 5 mm 1000 A Guard
£121.00
PL1251-1102 PL-SAX 1000A 10um 50 x 25mm Column PL-SAX 10 um 25.0 mm 50 mm 1000 A Column
£2,501.00
PL1251-3702 PL-SAX 1000A 30um 150 x 25mm Column PL-SAX 30 um 25.0 mm 150 mm 1000 A Column
£2,731.00
PL1551-3702 PL-SAX 1000A 30um 150 x 4.6mm Column PL-SAX 30 um 4.6 mm 150 mm 1000 A Column
£575.00
PL1751-3702 PL-SAX 1000A 30um 150 x 50mm Column PL-SAX 30 um 50.0 mm 150 mm 1000 A Column
£9,204.00
PL1551-5702 PL-SAX 1000A 30um 250 x 4.6mm Column PL-SAX 30 um 4.6 mm 250 mm 1000 A Column
£694.00
PL1851-3102 PL-SAX 1000A 30um 300 x 100mm Column PL-SAX 30 um 100.0 mm 300 mm 1000 A Column
£27,352.00
PL1951-1502 PL-SAX 1000A 5um 50 x 2.1mm Column PL-SAX 5 um 2.1 mm 50 mm 1000 A Column
£603.00
PL1551-1502 PL-SAX 1000A 5um 50 x 4.6mm Column PL-SAX 5 um 4.6 mm 50 mm 1000 A Column
£603.00
PL1951-3802 PL-SAX 1000A 8um 150 x 2.1mm Column PL-SAX 8 um 2.1 mm 150 mm 1000 A Column
£721.00
PL1251-3802 PL-SAX 1000A 8um 150 x 25mm Column PL-SAX 8 um 25.0 mm 150 mm 1000 A Column
£3,488.00
PL1551-3802 PL-SAX 1000A 8um 150 x 4.6mm Column PL-SAX 8 um 4.6 mm 150 mm 1000 A Column
£721.00
PL1751-3802 PL-SAX 1000A 8um 150 x 50mm Column PL-SAX 8 um 50.0 mm 150 mm 1000 A Column
£11,411.00
PL1151-3802 PL-SAX 1000A 8um 150 x 7.5mm Column PL-SAX 8 um 7.5 mm 150 mm 1000 A Column
£1,176.00
PL1951-1802 PL-SAX 1000A 8um 50 x 2.1mm Column PL-SAX 8 um 2.1 mm 50 mm 1000 A Column
£557.00
PL1551-1802 PL-SAX 1000A 8um 50 x 4.6mm Column PL-SAX 8 um 4.6 mm 50 mm 1000 A Column
£557.00
PL1151-1802 PL-SAX 1000A 8um 50 x 7.5mm Column PL-SAX 8 um 7.5 mm 50 mm 1000 A Column
£896.00
PL1251-3103 PL-SAX 4000A 10um 150 x 25mm Column PL-SAX 10 um 25.0 mm 150 mm 4000 A Column
£3,249.00
PL1551-3103 PL-SAX 4000A 10um 150 x 4.6mm Column PL-SAX 10 um 4.6 mm 150 mm 4000 A Column
£684.00
PL1751-3103 PL-SAX 4000A 10um 150 x 50mm Column PL-SAX 10 um 50.0 mm 150 mm 4000 A Column
£10,863.00
PL1551-5103 PL-SAX 4000A 10um 250 x 4.6mm Column PL-SAX 10 um 4.6 mm 250 mm 4000 A Column
£812.00
PL1851-2103 PL-SAX 4000A 10um 300 x 100mm Column PL-SAX 10 um 100.0 mm 300 mm 4000 A Column
£30,478.00
PL1251-1103 PL-SAX 4000A 10um 50 x 25mm Column PL-SAX 10 um 25.0 mm 50 mm 4000 A Column
£2,501.00
PL1251-3703 PL-SAX 4000A 30um 150 x 25mm Column PL-SAX 30 um 25.0 mm 150 mm 4000 A Column
£2,731.00
PL1551-3703 PL-SAX 4000A 30um 150 x 4.6mm Column PL-SAX 30 um 4.6 mm 150 mm 4000 A Column
£575.00
PL1751-3703 PL-SAX 4000A 30um 150 x 50mm Column PL-SAX 30 um 50.0 mm 150 mm 4000 A Column
£9,204.00
PL1551-5703 PL-SAX 4000A 30um 250 x 4.6mm Column PL-SAX 30 um 4.6 mm 250 mm 4000 A Column
£694.00
PL1851-3103 PL-SAX 4000A 30um 300 x 100mm Column PL-SAX 30 um 100.0 mm 300 mm 4000 A Column
£27,352.00
PL1951-1503 PL-SAX 4000A 5um 50 x 2.1mm Column PL-SAX 5 um 2.1 mm 50 mm 4000 A Column
£603.00
PL1551-1503 PL-SAX 4000A 5um 50 x 4.6mm Column PL-SAX 5 um 4.6 mm 50 mm 4000 A Column
£603.00
PL1951-3803 PL-SAX 4000A 8um 150 x 2.1mm Column PL-SAX 8 um 2.1 mm 150 mm 4000 A Column
£721.00
PL1551-3803 PL-SAX 4000A 8um 150 x 4.6mm Column PL-SAX 8 um 4.6 mm 150 mm 4000 A Column
£721.00
PL1151-3803 PL-SAX 4000A 8um 150 x 7.5mm Column PL-SAX 8 um 7.5 mm 150 mm 4000 A Column
£1,176.00
PL1951-1803 PL-SAX 4000A 8um 50 x 2.1mm Column PL-SAX 8 um 2.1 mm 50 mm 4000 A Column
£557.00
PL1551-1803 PL-SAX 4000A 8um 50 x 4.6mm Column PL-SAX 8 um 4.6 mm 50 mm 4000 A Column
£557.00
PL1151-1803 PL-SAX 4000A 8um 50 x 7.5mm Column PL-SAX 8 um 7.5 mm 50 mm 4000 A Column
£896.00
  • Anion-exchange purifications over a wider pH range extend applications
  • HPLC flow rates and rapid equilibration reduce purification cycle times
  • Small particles deliver excellent chromatographic performance
  • Wide range of particle sizes and two pore sizes for flexible analysis to scale up purification
  • 1000 Å pore size for high-capacity purifications
  • 4000 Å gigaporous particles with improved mass transfer for large biomolecules and high-speed, high-resolution purifications
  • Exceptional stability for long column lifetime
  PL-SAX
Phase Strong Anion Exchange
Particle Type Fully Porous PS/DVB particles
Particle Size 5 µm, 8 µm, 10 µm, and 30 µm
Pore Size 1000 Å and 4000 Å
pH Range 1 – 14
Maximum Temperature 80°C
Hardware Stainless Steel
Agilent Bio HPLC Columns and Consumables Catalogue (21.16 MB)
Your essential resource for biomolecule analysis
PL-SAX Oligonucleotide Workflow Ordering Guide (499.85 kB)
Oligonucleotide purification using anion exchange liquid chromatography
Charge Variant Analysis Application Compendium (10.49 MB)
Collection of Agilent app notes on charge variant analysis
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