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Agilent Sample Loops for HPLC

Agilent manufactures a range of high quality loop capillaries suitable for use with a variety of autosampling equipment. Stainless steel loop capillaries are available in 100 µL or 900 µL capacities, designed to deliver robust and high precision performance in various Agilent autosamplers. Other loop capillaries are available in 8, 20 or 40 µL with an even larger capacity 5 mL model also available. Agilent's loop capillaries are ideal replacement and spare parts for autosamplers and are manufactured to exacting quality standards in all areas.

Part Number Short Description Type Volume Pack Size Price Qty
5067-5424 20uL Loop 2D-LC 1
£168.00
5067-5425 40uL Loop 2D-LC 1
£179.00
5067-5437 60uL Loop 2D-LC 1
£174.00
5067-5426 80uL Loop 2D-LC 1
£189.00
G2258-60002 Buffer Loop Extension Assembly For G2258A 1
£241.00
G2258-87300 Buffer Loop Tubing Assembly, PTFE For G2258A 1
£57.00
5068-0035 Capillary Loop, 100uL, Stainless Steel For G2258A 100 uL 1
£227.00
5068-0051 Capillary Loop, 10uL, Stainless Steel For G2258A 10 uL 1
£227.00
5068-0033 Capillary Loop, 20uL, Stainless Steel For G2258A 20 uL 1
£227.00
5068-0031 Capillary Loop, 2uL, Stainless Steel For G2258A 2 uL 1
£227.00
5068-0034 Capillary Loop, 50uL, Stainless Steel For G2258A 50 uL 1
£227.00
5068-0032 Capillary Loop, 5uL, Stainless Steel For G2258A 5 uL 1
£227.00
G4277-60602 DLW 2 Holding Loop (Stainless Steel) Assembly For G4278A, G4277A 1
£852.00
G1329-87302 Extended Loop Capillary, 0.25mm x 180cm For G1389A 40 uL 1
£136.00
G7167-60301 Flex Extension Left Dual-Needle For G7167A/B 1
£280.00
G7167-60311 Flex Extension Right Dual-Needle For G7167A/B 1
£350.00
5067-4710 Flex Loop Kit, 100uL, Colour Code Blue For G1367E, G4226A 100 uL
£351.00
G4226-60310 Flex Loop Kit, 20uL, Colour Code Red For G1367E, G4226A 20 uL 1
£445.00
5067-4703 Flex Loop Kit, 40uL, Colour Code Green For G1367E, G4226A 40 uL
£351.00
G1375-87303 Fused Silica/PEEK Capillary, 100um x 110cm For G1389A 8 uL 1
£142.00
G1377-87310 Loop Capillary, 40uL For G1367D 40 uL 1
£343.00
G1377-87300 Loop Capillary, 40uL For G1377A 40 uL 1
£240.00
G1375-87315 Loop Capillary, 8uL For G1377A 8 uL 1
£142.00
01078-87302 Loop Capillary, Stainless Steel, 100uL For G1313A, G1329A/B, G2260A 100 uL 1
£159.00
G1367-87300 Loop Capillary, Stainless Steel, 100uL For G1367A/B/C 100 uL 1
£321.00
G1313-87303 Loop Extension Capillary, Stainless Steel, 900uL For G1313A, G1329A/B, G2260A 900 uL 1
£207.00
G4226-60415 Loop Flex Assembly, 40uL For G5667A 40 uL 1
£236.00
G7157-60515 Loop for Prep Autosampler For G7157A 1
£133.00
5188-6461 Loop, 100uL, Cheminert Injection Valve For G4278A, G4277A 100 uL 1
£77.00
5188-6458 Loop, 10uL, Cheminert Injection Valve For G4278A, G4277A 10 uL 1
£50.00
5188-6459 Loop, 20uL, Cheminert Injection Valve For G4278A, G4277A 20 uL 1
£50.00
5188-6462 Loop, 250uL, Cheminert Injection Valve For G4278A, G4277A 250 uL 1
£76.00
5188-6457 Loop, 2uL, Cheminert Injection Valve For G4278A, G4277A 2 uL 1
£51.00
5188-6468 Loop, PEEK 20uL, Cheminert Injection Valve For G4278A, G4277A 20 uL 1
£63.00
5188-6469 Loop, PEEK 2uL, Cheminert Injection Valve For G4278A, G4277A 2 uL 1
£70.00
G2260-68711 Multidraw Loop, 5 mL For G2260A 5 mL 1
£406.00
G7167-68711 Multidraw Upgrade Kit (No Dual Loop, Requires 900uL Option) For G1313A, G1329A/B, G7129A/B, G7167A/B
£302.00
G7129-60300 Sample Loop 20uL, Colour Code Red For G7129A/B 20 uL 1
£90.00
G7129-60400 Sample Loop 40uL, Colour Code Green For G7129A/B 40 uL 1
£87.00
G4267-60501 Sample Loop Flex, 100uL, Left, Dual Needle Option For G7167A/B 100 uL 1
£238.00
G4267-60511 Sample Loop Flex, 100uL, Right, Dual Needle Option For G7167A/B 100 uL 1
£323.00
G4267-60500 Sample Loop Flex, 100uL, Single Needle Option For G7167A/B 100 uL 1
£413.00
G4267-60301 Sample Loop Flex, 20uL, Left, Dual Needle Option For G7167A/B 20 uL 1
£210.00
G4267-60311 Sample Loop Flex, 20uL, Right, Dual Needle Option For G7167A/B 20 uL 1
£294.00
G4267-60300 Sample Loop Flex, 20uL, Single Needle Option For G7167A/B 20 uL 1
£361.00
G4267-60401 Sample Loop Flex, 40uL, Left, Dual Needle Option For G7167A/B 40 uL 1
£224.00
G4267-60411 Sample Loop Flex, 40uL, Right, Dual Needle Option For G7167A/B 40 uL 1
£281.00
G4267-60400 Sample Loop Flex, 40uL, Single Needle Option For G7167A/B 40 uL 1
£406.00
G7167-60300 Sample Loop Flex, 500-900uL, Right For G7167A/B 500 - 900 uL 1
£280.00
G7167-68501 Sample Loop Flex, 500uL, Left, High Volume Option, Dual Needle Option For G7167A/B 500 uL 1
£478.00
G7167-68511 Sample Loop Flex, 500uL, Right, High Volume Option, Dual Needle Option For G7167A/B 500 uL 1
£847.00
G7167-68500 Sample Loop Flex, 500uL, Single Needle Option For G7167A/B 500 uL 1
£468.00
G7167-68901 Sample Loop Flex, 900uL, Left, High Volume Option, Dual Needle Option For G7167A/B 900 uL 1
£503.00
G7167-68911 Sample Loop Flex, 900uL, Right, High Volume Option, Dual Needle Option For G7167A/B 900 uL 1
£881.00
G7167-68900 Sample Loop Flex, 900uL, Single Needle Option For G7167A/B 900 uL 1
£461.00
G7129-60500 Sample Loop, 100uL, Colour Code White For G7129A/B 100 uL 1
£68.00
G5668-60500 Sample Loop, Bio-inert, 100uL For G5668 100 uL 1
£1,082.00

Sample Loop Loading: Partial-filling vs. Complete-filling

Partial-filling

Use the partial-filling method if you need to conserve sample, or if you want to vary sample volume frequently.  In partial-filling, the syringe sets the volume injected onto the column. There is no sample waste, and the volume injected onto the column is equal to that dispensed from the syringe. Reproducibility is 1.0% relative standard deviation (RSD). The volume of the sample loaded is limited to half the sample loop volume. For example, the most you can load into a 200 µL sample loop is 100 µL. 
This limitation is due to the manner in which fluids move in tubes. Fluidic movement in tubes affects reproducibility.

Complete-filling

Use the complete-filling method if you have a sufficient amount of sample with which to work, you do not vary sample volume, or you need high reproducibility.  In complete-filling, the loop sets the volume loaded onto the column. You use excess sample (two to five in loop volumes) to replace all the mobile phase in the loop. 
Change the loop to vary the sample volume. Reproducibility is typically 0.1% RSD for loop sizes ³ 5 µL. Accuracy is limited as loop volumes are nominal.

”Which method should I use and which Rheodyne sample injectors use this method?”

There are two types of injectors available: dual mode and single mode. Dual mode injectors allow both partial- and complete-filling whereas single mode injectors allow only complete-filling.
If you are collecting experimental data, sample is scarce, and/or you want to use different sample volumes, a dual mode injector with a large volume sample loop is appropriate. Only dual mode injectors allow the partial-filling method with which you can easily vary your volumes (up to half your sample loop volume) by setting the syringe volume. Once you begin routine analysis, and/or you have an abundance of sample, either a dual mode or single mode injector is appropriate. Both types of injectors allow complete-filling method with which you fill the sample loop in excess. Complete-filling maximizes the reproducibility of your results.

InfinityLab LC Supplies Brochure (12.08 MB)
Consumable items for Agilent HPLC systems
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