Agilent ZORBAX 300Extend-C18 Columns

Zorbax 300Extend-C18 HPLC Columns from Agilent

Zorbax 300Extend-C18 is a wide-pore HPLC column for high efficiency separations of peptides, polypeptides, and small proteins from pH 2-11.5. Extend-C18 and 300Extend-C18 columns incorporate a unique patented bidentate silane, combined with a double-endcapping process that protects the silica from dissolution at high pH. At high pH, the retention and selectivity of peptides can change dramatically as a result of changes in amino acid charge.

Excellent recoveries of hydrophobic polypeptides have been achieved at high pH with the 300Extend C18. In addition, LC-MS sensitivity of peptides can also be improved at high pH using a simple ammonium hydroxide mobile phase. These conditions allow for ions to be detected in both positive and negative ion modes simultaneously with the 300Extend-C18. The Zorbax 300Extend-C18 is a rugged column and is ideal for LC-MS with ammonium hydroxide mobile phase.

Agilent ZORBAX 300Extend-C18 is a wide-pore HPLC column for high-efficiency separations of peptides from pH 2 - 11.5. The unique, bidentate bonded phase provides excellent lifetime and reproducibility at high and low pH. At high pH, retention and selectivity of peptides and polypeptides can change dramatically because of changes in charge on molecules. Excellent recoveries of hydrophobic polypeptides have been achieved at room temperature and high pH. LC/MS sensitivity of peptides and polypeptides can also be improved at high pH using a simple ammonium-hydroxide containing mobile phase.

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Buy

Part Number	Short Description	Phase Type	Phase	USP	Particle Size	ID	Length	Pore Size	Type	Price
761775-902	ZORBAX 300A Extend-C18 2.1 x 100mm 3.5um Column	C18	ZORBAX Extend-C18	L1	3.5 um	2.1 mm	100 mm	300 A	Column	£574.00
821125-932	ZORBAX 300A Extend-C18 2.1 x 12.5mm 5um Guard 4pk	C18	ZORBAX Extend-C18	L1	5 um	2.1 mm	12.5 mm	300 A	Guard	£208.00
763750-902	ZORBAX 300A Extend-C18 2.1 x 150mm 3.5um Column	C18	ZORBAX Extend-C18	L1	3.5 um	2.1 mm	150 mm	300 A	Column	£639.00
765750-902	ZORBAX 300A Extend-C18 2.1 x 50mm 3.5um Column	C18	ZORBAX Extend-C18	L1	3.5 um	2.1 mm	50 mm	300 A	Column	£427.00
761973-902	ZORBAX 300A Extend-C18 4.6 x 100mm 3.5um Column	C18	ZORBAX Extend-C18	L1	3.5 um	4.6 mm	100 mm	300 A	Column	£574.00
820950-932	ZORBAX 300A Extend-C18 4.6 x 12.5mm 5um Guard 4pk	C18	ZORBAX Extend-C18	L1	5 um	4.6 mm	12.5 mm	300 A	Guard	£208.00
763973-902	ZORBAX 300A Extend-C18 4.6 x 150mm 3.5um Column	C18	ZORBAX Extend-C18	L1	3.5 um	4.6 mm	150 mm	300 A	Column	£639.00
773995-902	ZORBAX 300A Extend-C18 4.6 x 150mm 5um Column	C18	ZORBAX Extend-C18	L1	5 um	4.6 mm	150 mm	300 A	Column	£573.00
770995-902	ZORBAX 300A Extend-C18 4.6 x 250mm 5um Column	C18	ZORBAX Extend-C18	L1	5 um	4.6 mm	250 mm	300 A	Column	£638.00
765973-902	ZORBAX 300A Extend-C18 4.6 x 50mm 3.5um Column	C18	ZORBAX Extend-C18	L1	3.5 um	4.6 mm	50 mm	300 A	Column	£427.00

Features

Rugged, high and low pH separations of peptides, polypeptides, and small proteins from pH 2 - 11.5
Different selectivity possible at high and low pH
High efficiency and good recovery of hydrophobic peptides at high pH
Ideal for LC-MS with ammonium-hydroxide-modified mobile phase

Specifications

	ZORBAX 300Extend-C18
Bonded Phase	C18
Particle Type	Fully Porous
Endcapped	Yes
Carbon Load	4%
Particle Size	3.5 µm, 5 µm
Pore Size	300 Å
Temperature Limit	60 °C up to pH 8, 40 °C from pH 8 - 11.5
pH Range	2 - 11.5
USP Designation	L1

Literature

AdvanceBio Reversed Phase Columns White Paper (3.07 MB)

Better characterization of biomolecules using Agilent AdvanceBio reversed phase columns

Agilent Bio HPLC Columns (26.00 MB)

Your essential resource for biomolecule analysis