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Daicel Protein-based Chiral Columns

Protein Based Chiral Columns from Daicel

Columns packed with the immobilized protein stationary phases (PSPs) are successfully utilized by scientists for separation and characterization of chiral compounds in a great number of applications, ranging from drug discovery and quality assurance of marketed drugs to environmental monitoring.

Our protein-based chiral selectors are immobilized on 5 µm spherical silica particles. The PSPs function entirely in the reversed-phase chromatographic mode, using buffers with low organic modifier content and at moderate pH values. Enantioselectivity can easily be controlled or improved by changes in the mobile phase composition: pH, buffer and organic modifier types and concentrations.

All PSP selectors are packed in columns with 2, 3, 4 and 10 mm internal diameter to cover separations on both analytical and semi-preparative scales.

Daicel CHIRALPAK protein-based chiral selectors are immobilised on 5 µm spherical silica particles. They operate in reversed phase mode, using buffers with low organic modifier content and at moderate pH values. Enantioselectivity can easily be controlled or improved by changes in the mobile phase composition.

Daicel protein stationary chiral selectors are available with three main proteins:
CHIRALPAK AGP – α 1-acid glycoprotein (USP L41)
CHIRALPAK HSA – human serum albumin (USP L79)
CHIRALPAK CBH – cellobiohydrolase (stable enzyme) (USP L108)

The Daicel CHIRALPAK AGP selector has extremely broad applicability for the separation of a wide variety of chiral compounds, such as amines, acids, and nonprolytes. The enantioselectivity of the Daicel CHIRALPAK CBH selector is complementary to that of the AGP selector.

A range of other serum albumin protein columns is available for use alongside CHIRALPAK HSA in binding studies to confirm the ability of a pharmaceutical to bind to a protein, including:
CHIRALPAK RSA – rat serum albumin
CHIRALPAK BSA – bovine serum albumin (USP L75)
CHIRALPAK MSA – mouse serum albumin
CHIRALPAK PSA – pig serum albumin
CHIRALPAK DSA – dog serum albumin

Part Number Short Description Phase Particle Size ID Length Type Price Qty
30791 CHIRALPAK AGP 10 x 2mm 5um Guard Cartridge, 2pk CHIRALPAK AGP 5 um 2.0 mm 10 mm Guard
£222.79
30781 CHIRALPAK AGP 10 x 3mm 5um Guard Cartridge, 2pk CHIRALPAK AGP 5 um 3.0 mm 10 mm Guard
£222.79
30711 CHIRALPAK AGP 10 x 4mm 5um Guard Cartridge, 2pk CHIRALPAK AGP 5 um 4.0 mm 10 mm Guard
£255.97
30733 CHIRALPAK AGP 100 x 10mm 5um Semi-Prep Column CHIRALPAK AGP 5 um 10.0 mm 100 mm Semi-Prep Column
£4,299.75
30793 CHIRALPAK AGP 100 x 2.1mm 5um Analytical Column CHIRALPAK AGP 5 um 2.1 mm 100 mm Analytical Column
£1,355.71
30783 CHIRALPAK AGP 100 x 3mm 5um Analytical Column CHIRALPAK AGP 5 um 3.0 mm 100 mm Analytical Column
£1,232.47
30713 CHIRALPAK AGP 100 x 4mm 5um Analytical Column CHIRALPAK AGP 5 um 4.0 mm 100 mm Analytical Column
£1,232.47
30734 CHIRALPAK AGP 150 x 10mm 5um Semi-Prep Column CHIRALPAK AGP 5 um 10.0 mm 150 mm Semi-Prep Column
£6,252.89
30794 CHIRALPAK AGP 150 x 2.1mm 5um Analytical Column CHIRALPAK AGP 5 um 2.1 mm 150 mm Analytical Column
£1,772.86
30784 CHIRALPAK AGP 150 x 3mm 5um Analytical Column CHIRALPAK AGP 5 um 3.0 mm 150 mm Analytical Column
£1,616.43
30714 CHIRALPAK AGP 150 x 4mm 5um Analytical Column CHIRALPAK AGP 5 um 4.0 mm 150 mm Analytical Column
£1,616.43
30792 CHIRALPAK AGP 50 x 2.1mm 5um Analytical Column CHIRALPAK AGP 5 um 2.1 mm 50 mm Analytical Column
£1,061.82
30782 CHIRALPAK AGP 50 x 3mm 5um Analytical Column CHIRALPAK AGP 5 um 3.0 mm 50 mm Analytical Column
£971.75
30712 CHIRALPAK AGP 50 x 4mm 5um Analytical Column CHIRALPAK AGP 5 um 4.0 mm 50 mm Analytical Column
£971.75
35714 CHIRALPAK BSA 150 x 4mm 5um Analytical Column CHIRALPAK BSA 5 um 4.0 mm 150 mm Analytical Column
£2,682.99
35792 CHIRALPAK BSA 50 x 2.1mm 5um Analytical Column CHIRALPAK BSA 5 um 2.1 mm 50 mm Analytical Column
£1,635.39
35782 CHIRALPAK BSA 50 x 3mm 5um Analytical Column CHIRALPAK BSA 5 um 3.0 mm 50 mm Analytical Column
£1,635.39
35712 CHIRALPAK BSA 50 x 4mm 5um Analytical Column CHIRALPAK BSA 5 um 4.0 mm 50 mm Analytical Column
£1,635.39
33791 CHIRALPAK CBH 10 x 2mm 5um Guard Cartridge, 2pk CHIRALPAK CBH 5 um 2.0 mm 10 mm Guard
£222.79
33781 CHIRALPAK CBH 10 x 3mm 5um Guard Cartridge, 2pk CHIRALPAK CBH 5 um 3.0 mm 10 mm Guard
£222.79
33711 CHIRALPAK CBH 10 x 4mm 5um Guard Cartridge, 2pk CHIRALPAK CBH 5 um 4.0 mm 10 mm Guard
£255.97
33733 CHIRALPAK CBH 100 x 10mm 5um Semi-Prep Column CHIRALPAK CBH 5 um 10.0 mm 100 mm Semi-Prep Column
£4,299.75
33793 CHIRALPAK CBH 100 x 2.1mm 5um Analytical Column CHIRALPAK CBH 5 um 2.1 mm 100 mm Analytical Column
£1,355.71
33783 CHIRALPAK CBH 100 x 3mm 5um Analytical Column CHIRALPAK CBH 5 um 3.0 mm 100 mm Analytical Column
£1,232.47
33713 CHIRALPAK CBH 100 x 4mm 5um Analytical Column CHIRALPAK CBH 5 um 4.0 mm 100 mm Analytical Column
£1,232.47
33734 CHIRALPAK CBH 150 x 10mm 5um Semi-Prep Column CHIRALPAK CBH 5 um 10.0 mm 150 mm Semi-Prep Column
£6,252.89
33794 CHIRALPAK CBH 150 x 2.1mm 5um Analytical Column CHIRALPAK CBH 5 um 2.1 mm 150 mm Analytical Column
£1,772.86
33784 CHIRALPAK CBH 150 x 3mm 5um Analytical Column CHIRALPAK CBH 5 um 3.0 mm 150 mm Analytical Column
£1,616.43
33714 CHIRALPAK CBH 150 x 4mm 5um Analytical Column CHIRALPAK CBH 5 um 4.0 mm 150 mm Analytical Column
£1,616.43
33792 CHIRALPAK CBH 50 x 2.1mm 5um Analytical Column CHIRALPAK CBH 5 um 2.1 mm 50 mm Analytical Column
£1,061.82
33782 CHIRALPAK CBH 50 x 3mm 5um Analytical Column CHIRALPAK CBH 5 um 3.0 mm 50 mm Analytical Column
£971.75
33712 CHIRALPAK CBH 50 x 4mm 5um Analytical Column CHIRALPAK CBH 5 um 4.0 mm 50 mm Analytical Column
£971.75
36714 CHIRALPAK DSA 150 x 4mm 5um Analytical Column CHIRALPAK DSA 5 um 4.0 mm 150 mm Analytical Column
£5,712.01
36792 CHIRALPAK DSA 50 x 2.1mm 5um Analytical Column CHIRALPAK DSA 5 um 2.1 mm 50 mm Analytical Column
£2,962.66
36782 CHIRALPAK DSA 50 x 3mm 5um Analytical Column CHIRALPAK DSA 5 um 3.0 mm 50 mm Analytical Column
£2,962.66
36712 CHIRALPAK DSA 50 x 4mm 5um Analytical Column CHIRALPAK DSA 5 um 4.0 mm 50 mm Analytical Column
£3,597.86
34791 CHIRALPAK HSA 10 x 2mm 5um Guard Cartridge, 2pk CHIRALPAK HSA 5 um 2.0 mm 10 mm Guard
£203.83
34781 CHIRALPAK HSA 10 x 3mm 5um Guard Cartridge, 2pk CHIRALPAK HSA 5 um 3.0 mm 10 mm Guard
£203.83
34711 CHIRALPAK HSA 10 x 4mm 5um Guard Cartridge, 2pk CHIRALPAK HSA 5 um 4.0 mm 10 mm Guard
£237.01
34733 CHIRALPAK HSA 100 x 10mm 5um Semi-Prep Column CHIRALPAK HSA 5 um 10.0 mm 100 mm Semi-Prep Column
£4,299.75
34793 CHIRALPAK HSA 100 x 2.1mm 5um Analytical Column CHIRALPAK HSA 5 um 2.1 mm 100 mm Analytical Column
£1,161.36
34783 CHIRALPAK HSA 100 x 3mm 5um Analytical Column CHIRALPAK HSA 5 um 3.0 mm 100 mm Analytical Column
£1,038.12
34713 CHIRALPAK HSA 100 x 4mm 5um Analytical Column CHIRALPAK HSA 5 um 4.0 mm 100 mm Analytical Column
£1,038.12
34734 CHIRALPAK HSA 150 x 10mm 5um Semi-Prep Column CHIRALPAK HSA 5 um 10.0 mm 150 mm Semi-Prep Column
£6,252.89
34794 CHIRALPAK HSA 150 x 2.1mm 5um Analytical Column CHIRALPAK HSA 5 um 2.1 mm 150 mm Analytical Column
£1,426.82
34784 CHIRALPAK HSA 150 x 3mm 5um Analytical Column CHIRALPAK HSA 5 um 3.0 mm 150 mm Analytical Column
£1,322.53
34714 CHIRALPAK HSA 150 x 4mm 5um Analytical Column CHIRALPAK HSA 5 um 4.0 mm 150 mm Analytical Column
£1,322.53
34792 CHIRALPAK HSA 50 x 2.1mm 5um Analytical Column CHIRALPAK HSA 5 um 2.1 mm 50 mm Analytical Column
£971.75
34782 CHIRALPAK HSA 50 x 3mm 5um Analytical Column CHIRALPAK HSA 5 um 3.0 mm 50 mm Analytical Column
£886.43
34712 CHIRALPAK HSA 50 x 4mm 5um Analytical Column CHIRALPAK HSA 5 um 4.0 mm 50 mm Analytical Column
£886.43
37782 CHIRALPAK MSA 50 x 3mm 5um Analytical Column CHIRALPAK MSA 5 um 3.0 mm 50 mm Analytical Column
£2,948.44
37712 CHIRALPAK MSA 50 x 4mm 5um Analytical Column CHIRALPAK MSA 5 um 4.0 mm 50 mm Analytical Column
£3,597.86
43782 CHIRALPAK PSA 50 x 3mm 5um Analytical Column CHIRALPAK PSA 5 um 3.0 mm 50 mm Analytical Column
£2,948.44
43712 CHIRALPAK PSA 50 x 4mm 5um Analytical Column CHIRALPAK PSA 5 um 4.0 mm 50 mm Analytical Column
£3,597.86
38714 CHIRALPAK RSA 150 x 4mm 5um Analytical Column CHIRALPAK RSA 5 um 4.0 mm 150 mm Analytical Column
£3,597.86
38792 CHIRALPAK RSA 50 x 2.1mm 5um Analytical Column CHIRALPAK RSA 5 um 2.1 mm 50 mm Analytical Column
£1,796.56
38782 CHIRALPAK RSA 50 x 3mm 5um Analytical Column CHIRALPAK RSA 5 um 3.0 mm 50 mm Analytical Column
£1,796.56
38712 CHIRALPAK RSA 50 x 4mm 5um Analytical Column CHIRALPAK RSA 5 um 4.0 mm 50 mm Analytical Column
£1,796.56
  • CHIRALPAK AGP has very broad applicability and is suitable for enantiomer resolution of all types of compounds, including amines (primary, secondary, tertiary, and quaternary ammonium compounds), strong and weak acids, and non-ionisable compounds.
  • CHIRALPAK HSA offers high enantioselectivity for compounds bearing carboxylic groups, including, strong and weak acids, zwitterionic molecules, and non-ionisable compounds.
  • CHIRALPAK CBH can offer high enantioselectivity for basic compounds, including primary and secondary amines, and non-ionisable compounds.
  CHIRALPAK AGP CHIRALPAK HSA CHIRALPAK CBH 
Chiral selector α 1-acid glycoprotein human serum albumin cellobiohydrolase (stable enzyme) 
USP L41 L79 L108
Particle size 5 µm 5 µm 5 µm
Type Immobilised protein-based Immobilised protein-based Immobilised protein-based
Separation mode revesed phase revesed phase revesed phase
Shipping solvent water/2-propanol (85/15) water/2-propanol (90/10) water/2-propanol (85/15)
Temperature range 20 - 30°C 20 - 30°C 20 - 30°C
Daicel Chiral Selectors Poster (279.53 kB)
Structures of commercially available Daicel chiral stationary phases
Daicel Product List 2024 (2.97 MB)
Catalogue of part numbers for Daicel columns
CHIRALPAK AGP, HSA and CBH Brochure (5.46 MB)
Immobilized protein stationary phases
CHIRALPAK AGP, CBH and HSA Screening Strategy (63.97 kB)
Screening Strategies for immobilised protein stationary phases to identify the best method to separate target compounds
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