HALO® BioClass C4 HPLC Columns from Advanced Materials Technology

With both tailored particle and pore size options, HALO^® BioClass C4 offers application specific solutions for proteins. The densely bonded, extensively endcapped dimethylbutyl stationary phase of HALO^® C4 provides a stable, reversed-phase packing that can be used for separating high molecular weight compounds such as proteins.

HALO 1000 Å C4 is based on a wide-pore 1000 Å Fused-Core^® particle design. This particle exhibits very high column efficiency due to the shallow diffusion paths in the 0.5 µm thin porous shell and the small overall particle size of 2.7 µm.

HALO 400 Å C4 is based on a wide-pore 400 Å Fused-Core^® particle design. This particle exhibits very high column efficiency due to the shallow diffusion paths in the 0.2 µm thin porous shell and the small overall particle size of 3.4 µm.

HALO® BioClass C4 columns from Advanced Materials Technology offer application specific solutions for proteins with tailored particle and pore size options. The densely bonded, extensively endcapped dimethylbutyl stationary phase of HALO® C4 provides a stable, reversed-phase packing that can be used for separating high molecular weight compounds.

The HALO® C4 columns are available in a 1000 Å 2.7 µm Fused-Core® particle and a 400 Å 3.4 µm Fused-Core® particle. The 1000 Å particle is used for the ultimate resolution of mAbs, while the 400 Å particle is designed for fast analysis of large proteins at lower back pressures.

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Part Number	Short Description	Phase Type	Phase	USP	Particle Size	ID	Length	Pore Size	Type	Price
97216-614	HALO 1000A C4, 2.7um, 0.3 x 100mm	C4	HALO C4	L26	2.7 um	0.3 mm	100 mm	1000 A	Column	£906.52
97216-714	HALO 1000A C4, 2.7um, 0.3 x 150mm	C4	HALO C4	L26	2.7 um	0.3 mm	150 mm	1000 A	Column	£906.52
97216-414	HALO 1000A C4, 2.7um, 0.3 x 50mm	C4	HALO C4	L26	2.7 um	0.3 mm	50 mm	1000 A	Column	£820.89
97215-614	HALO 1000A C4, 2.7um, 0.5 x 100mm	C4	HALO C4	L26	2.7 um	0.5 mm	100 mm	1000 A	Column	£906.52
97215-714	HALO 1000A C4, 2.7um, 0.5 x 150mm	C4	HALO C4	L26	2.7 um	0.5 mm	150 mm	1000 A	Column	£906.52
97215-414	HALO 1000A C4, 2.7um, 0.5 x 50mm	C4	HALO C4	L26	2.7 um	0.5 mm	50 mm	1000 A	Column	£820.89
92711-614	HALO 1000A C4, 2.7um, 1 x 100mm	C4	HALO C4	L26	2.7 um	1.0 mm	100 mm	1000 A	Column	£726.92
92711-714	HALO 1000A C4, 2.7um, 1 x 150mm	C4	HALO C4	L26	2.7 um	1.0 mm	150 mm	1000 A	Column	£790.98
92711-414	HALO 1000A C4, 2.7um, 1 x 50mm	C4	HALO C4	L26	2.7 um	1.0 mm	50 mm	1000 A	Column	£654.11
9271X-614	HALO 1000A C4, 2.7um, 1.5 x 100mm	C4	HALO C4	L26	2.7 um	1.5 mm	100 mm	1000 A	Column	£820.89
9271X-714	HALO 1000A C4, 2.7um, 1.5 x 150mm	C4	HALO C4	L26	2.7 um	1.5 mm	150 mm	1000 A	Column	£910.78
9271X-414	HALO 1000A C4, 2.7um, 1.5 x 50mm	C4	HALO C4	L26	2.7 um	1.5 mm	50 mm	1000 A	Column	£744.01
92710-614	HALO 1000A C4, 2.7um, 10 x 100mm	C4	HALO C4	L26	2.7 um	10.0 mm	100 mm	1000 A	Column	£2,308.69
92710-714	HALO 1000A C4, 2.7um, 10 x 150mm	C4	HALO C4	L26	2.7 um	10.0 mm	150 mm	1000 A	Column	£2,552.36
92710-414	HALO 1000A C4, 2.7um, 10 x 50mm	C4	HALO C4	L26	2.7 um	10.0 mm	50 mm	1000 A	Column	£1,923.88
92712-614	HALO 1000A C4, 2.7um, 2.1 x 100mm	C4	HALO C4	L26	2.7 um	2.1 mm	100 mm	1000 A	Column	£820.89
92712-714	HALO 1000A C4, 2.7um, 2.1 x 150mm	C4	HALO C4	L26	2.7 um	2.1 mm	150 mm	1000 A	Column	£910.78
92712-214	HALO 1000A C4, 2.7um, 2.1 x 20mm	C4	HALO C4	L26	2.7 um	2.1 mm	20 mm	1000 A	Column	£744.01
92712-914	HALO 1000A C4, 2.7um, 2.1 x 250mm	C4	HALO C4	L26	2.7 um	2.1 mm	250 mm	1000 A	Column	£1,051.73
92712-314	HALO 1000A C4, 2.7um, 2.1 x 30mm	C4	HALO C4	L26	2.7 um	2.1 mm	30 mm	1000 A	Column	£744.01
92712-414	HALO 1000A C4, 2.7um, 2.1 x 50mm	C4	HALO C4	L26	2.7 um	2.1 mm	50 mm	1000 A	Column	£744.01
92712-114	HALO 1000A C4, 2.7um, 2.1 x 5mm, Guard Column 3pk	C4	HALO C4	L26	2.7 um	2.1 mm	5 mm	1000 A	Guard	£525.97
92712-514	HALO 1000A C4, 2.7um, 2.1 x 75mm	C4	HALO C4	L26	2.7 um	2.1 mm	75 mm	1000 A	Column	£778.17
92713-614	HALO 1000A C4, 2.7um, 3 x 100mm	C4	HALO C4	L26	2.7 um	3.0 mm	100 mm	1000 A	Column	£820.89
92713-714	HALO 1000A C4, 2.7um, 3 x 150mm	C4	HALO C4	L26	2.7 um	3.0 mm	150 mm	1000 A	Column	£910.78
92713-214	HALO 1000A C4, 2.7um, 3 x 20mm	C4	HALO C4	L26	2.7 um	3.0 mm	20 mm	1000 A	Column	£744.01
92713-914	HALO 1000A C4, 2.7um, 3 x 250mm	C4	HALO C4	L26	2.7 um	3.0 mm	250 mm	1000 A	Column	£1,051.73
92713-314	HALO 1000A C4, 2.7um, 3 x 30mm	C4	HALO C4	L26	2.7 um	3.0 mm	30 mm	1000 A	Column	£744.01
92713-414	HALO 1000A C4, 2.7um, 3 x 50mm	C4	HALO C4	L26	2.7 um	3.0 mm	50 mm	1000 A	Column	£744.01
92713-114	HALO 1000A C4, 2.7um, 3 x 5mm, Guard Column 3pk	C4	HALO C4	L26	2.7 um	3.0 mm	5 mm	1000 A	Guard	£525.97
92713-514	HALO 1000A C4, 2.7um, 3 x 75mm	C4	HALO C4	L26	2.7 um	3.0 mm	75 mm	1000 A	Column	£778.17
92714-614	HALO 1000A C4, 2.7um, 4.6 x 100mm	C4	HALO C4	L26	2.7 um	4.6 mm	100 mm	1000 A	Column	£820.89
92714-714	HALO 1000A C4, 2.7um, 4.6 x 150mm	C4	HALO C4	L26	2.7 um	4.6 mm	150 mm	1000 A	Column	£910.78
92714-214	HALO 1000A C4, 2.7um, 4.6 x 20mm	C4	HALO C4	L26	2.7 um	4.6 mm	20 mm	1000 A	Column	£744.01
92714-914	HALO 1000A C4, 2.7um, 4.6 x 250mm	C4	HALO C4	L26	2.7 um	4.6 mm	250 mm	1000 A	Column	£1,051.73
92714-314	HALO 1000A C4, 2.7um, 4.6 x 30mm	C4	HALO C4	L26	2.7 um	4.6 mm	30 mm	1000 A	Column	£744.01
92714-414	HALO 1000A C4, 2.7um, 4.6 x 50mm	C4	HALO C4	L26	2.7 um	4.6 mm	50 mm	1000 A	Column	£744.01
92714-114	HALO 1000A C4, 2.7um, 4.6 x 5mm, Guard Column 3pk	C4	HALO C4	L26	2.7 um	4.6 mm	5 mm	1000 A	Guard	£525.97
92714-514	HALO 1000A C4, 2.7um, 4.6 x 75mm	C4	HALO C4	L26	2.7 um	4.6 mm	75 mm	1000 A	Column	£778.17
94316-614	HALO 400A C4, 3.4um, 0.3 x 100mm	C4	HALO C4	L26	3.4 um	0.3 mm	100 mm	400 A	Column	£906.52
94316-714	HALO 400A C4, 3.4um, 0.3 x 150mm	C4	HALO C4	L26	3.4 um	0.3 mm	150 mm	400 A	Column	£906.52
94316-414	HALO 400A C4, 3.4um, 0.3 x 50mm	C4	HALO C4	L26	3.4 um	0.3 mm	50 mm	400 A	Column	£820.89
94315-614	HALO 400A C4, 3.4um, 0.5 x 100mm	C4	HALO C4	L26	3.4 um	0.5 mm	100 mm	400 A	Column	£906.52
94315-714	HALO 400A C4, 3.4um, 0.5 x 150mm	C4	HALO C4	L26	3.4 um	0.5 mm	150 mm	400 A	Column	£906.52
94315-414	HALO 400A C4, 3.4um, 0.5 x 50mm	C4	HALO C4	L26	3.4 um	0.5 mm	50 mm	400 A	Column	£820.89
93411-614	HALO 400A C4, 3.4um, 1 x 100mm	C4	HALO C4	L26	3.4 um	1.0 mm	100 mm	400 A	Column	£726.92
93411-714	HALO 400A C4, 3.4um, 1 x 150mm	C4	HALO C4	L26	3.4 um	1.0 mm	150 mm	400 A	Column	£790.98
93411-414	HALO 400A C4, 3.4um, 1 x 50mm	C4	HALO C4	L26	3.4 um	1.0 mm	50 mm	400 A	Column	£654.11
93410-614	HALO 400A C4, 3.4um, 10 x 100mm	C4	HALO C4	L26	3.4 um	10.0 mm	100 mm	400 A	Column	£2,308.69
93410-714	HALO 400A C4, 3.4um, 10 x 150mm	C4	HALO C4	L26	3.4 um	10.0 mm	150 mm	400 A	Column	£2,552.36
93410-414	HALO 400A C4, 3.4um, 10 x 50mm	C4	HALO C4	L26	3.4 um	10.0 mm	50 mm	400 A	Column	£1,923.88
93412-614	HALO 400A C4, 3.4um, 2.1 x 100mm	C4	HALO C4	L26	3.4 um	2.1 mm	100 mm	400 A	Column	£705.56
93412-714	HALO 400A C4, 3.4um, 2.1 x 150mm	C4	HALO C4	L26	3.4 um	2.1 mm	150 mm	400 A	Column	£778.17
93412-214	HALO 400A C4, 3.4um, 2.1 x 20mm	C4	HALO C4	L26	3.4 um	2.1 mm	20 mm	400 A	Column	£560.14
93412-914	HALO 400A C4, 3.4um, 2.1 x 250mm	C4	HALO C4	L26	3.4 um	2.1 mm	250 mm	400 A	Column	£974.85
93412-314	HALO 400A C4, 3.4um, 2.1 x 30mm	C4	HALO C4	L26	3.4 um	2.1 mm	30 mm	400 A	Column	£560.14
93412-414	HALO 400A C4, 3.4um, 2.1 x 50mm	C4	HALO C4	L26	3.4 um	2.1 mm	50 mm	400 A	Column	£637.02
93412-114	HALO 400A C4, 3.4um, 2.1 x 5mm, Guard Column 3pk	C4	HALO C4	L26	3.4 um	2.1 mm	5 mm	400 A	Guard	£525.97
93412-514	HALO 400A C4, 3.4um, 2.1 x 75mm	C4	HALO C4	L26	3.4 um	2.1 mm	75 mm	400 A	Column	£697.03
93413-614	HALO 400A C4, 3.4um, 3 x 100mm	C4	HALO C4	L26	3.4 um	3.0 mm	100 mm	400 A	Column	£705.56
93413-714	HALO 400A C4, 3.4um, 3 x 150mm	C4	HALO C4	L26	3.4 um	3.0 mm	150 mm	400 A	Column	£778.17
93413-214	HALO 400A C4, 3.4um, 3 x 20mm	C4	HALO C4	L26	3.4 um	3.0 mm	20 mm	400 A	Column	£560.14
93413-914	HALO 400A C4, 3.4um, 3 x 250mm	C4	HALO C4	L26	3.4 um	3.0 mm	250 mm	400 A	Column	£974.85
93413-314	HALO 400A C4, 3.4um, 3 x 30mm	C4	HALO C4	L26	3.4 um	3.0 mm	30 mm	400 A	Column	£560.14
93413-414	HALO 400A C4, 3.4um, 3 x 50mm	C4	HALO C4	L26	3.4 um	3.0 mm	50 mm	400 A	Column	£637.02
93413-114	HALO 400A C4, 3.4um, 3 x 5mm, Guard Column 3pk	C4	HALO C4	L26	3.4 um	3.0 mm	5 mm	400 A	Guard	£525.97
93413-514	HALO 400A C4, 3.4um, 3 x 75mm	C4	HALO C4	L26	3.4 um	3.0 mm	75 mm	400 A	Column	£697.03
93414-614	HALO 400A C4, 3.4um, 4.6 x 100mm	C4	HALO C4	L26	3.4 um	4.6 mm	100 mm	400 A	Column	£705.56
93414-714	HALO 400A C4, 3.4um, 4.6 x 150mm	C4	HALO C4	L26	3.4 um	4.6 mm	150 mm	400 A	Column	£778.17
93414-214	HALO 400A C4, 3.4um, 4.6 x 20mm	C4	HALO C4	L26	3.4 um	4.6 mm	20 mm	400 A	Column	£560.14
93414-914	HALO 400A C4, 3.4um, 4.6 x 250mm	C4	HALO C4	L26	3.4 um	4.6 mm	250 mm	400 A	Column	£974.85
93414-314	HALO 400A C4, 3.4um, 4.6 x 30mm	C4	HALO C4	L26	3.4 um	4.6 mm	30 mm	400 A	Column	£560.14
93414-414	HALO 400A C4, 3.4um, 4.6 x 50mm	C4	HALO C4	L26	3.4 um	4.6 mm	50 mm	400 A	Column	£637.02
93414-114	HALO 400A C4, 3.4um, 4.6 x 5mm, Guard Column 3pk	C4	HALO C4	L26	3.4 um	4.6 mm	5 mm	400 A	Guard	£525.97
93414-514	HALO 400A C4, 3.4um, 4.6 x 75mm	C4	HALO C4	L26	3.4 um	4.6 mm	75 mm	400 A	Column	£697.03

Features

HALO® 1000 Å C4 is ideal for mAb analysis and offers best in class resolution for full characterization
HALO® 400 Å C4 is suitable for large protein analysis and is well suited for high speed separations
Alternative selectivity to HALO® ES-C18 and HALO® Diphenyl phases
USP L26 designation
Available particle sizes of 2.7 µm for 1000 Å pore size and 3.4 µm for 400 Å pore size

Specifications

	HALO® 400 Å C4	HALO® 1000 Å C4
Phase	Dimethylbutylsilane	Dimethylbutylsilane
Particle Type	Fused-Core®	Fused-Core®
Particle Size	3.4 µm	2.7 µm
Pore Size	400 Å	1000 Å
Endcapped	Yes	Yes
Low pH/Temperature Limit	2/90⁰C	2/90⁰C
High pH/Temperature Limit	9/40⁰C	9/40⁰C
Hardware	Stainless Steel	Stainless Steel
USP Designation	L26	L26

Literature

HALO Catalogue (11.13 MB)

A better path to separations with Fused-Core®

HALO® Bioclass Catalogue (5.65 MB)

HALO® columns for protein, peptide and glycan applications